ID   RL27A_TETTH             Reviewed;         149 AA.
AC   Q00454;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=60S ribosomal protein L27a;
GN   Name=RPL27A; Synonyms=RPL28;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=1946363; DOI=10.1073/pnas.88.21.9493;
RA   Yao M.-C., Yao C.-H.;
RT   "Transformation of Tetrahymena to cycloheximide resistance with a ribosomal
RT   protein gene through sequence replacement.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:9493-9497(1991).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (3.52 ANGSTROMS) OF 60S RIBOSOME.
RX   PubMed=22052974; DOI=10.1126/science.1211204;
RA   Klinge S., Voigts-Hoffmann F., Leibundgut M., Arpagaus S., Ban N.;
RT   "Crystal structure of the eukaryotic 60S ribosomal subunit in complex with
RT   initiation factor 6.";
RL   Science 334:941-948(2011).
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL15 family.
CC       {ECO:0000305}.
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DR   EMBL; M76718; AAA30124.1; -; mRNA.
DR   EMBL; M76719; AAA30125.1; -; Genomic_DNA.
DR   PIR; A56403; A56403.
DR   PDB; 4V8P; X-ray; 3.52 A; BK/CK/EK/GK=1-149.
DR   PDBsum; 4V8P; -.
DR   AlphaFoldDB; Q00454; -.
DR   SMR; Q00454; -.
DR   IntAct; Q00454; 1.
DR   GO; GO:0015934; C:large ribosomal subunit; IEA:InterPro.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006412; P:translation; IEA:InterPro.
DR   Gene3D; 4.10.990.10; -; 1.
DR   HAMAP; MF_01341; Ribosomal_L15; 1.
DR   InterPro; IPR036227; L18e/L15P_sf.
DR   InterPro; IPR027386; Rbsml_prot_L15/27a_N.
DR   InterPro; IPR030878; Ribosomal_L15.
DR   InterPro; IPR001196; Ribosomal_L15_CS.
DR   InterPro; IPR021131; Ribosomal_L18e/L15P.
DR   Pfam; PF00828; Ribosomal_L27A; 1.
DR   SUPFAM; SSF52080; SSF52080; 1.
DR   PROSITE; PS00475; RIBOSOMAL_L15; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Ribonucleoprotein; Ribosomal protein.
FT   CHAIN           1..149
FT                   /note="60S ribosomal protein L27a"
FT                   /id="PRO_0000104895"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         40
FT                   /note="M->K,E: Confers cycloheximide resistance."
FT   CONFLICT        82..85
FT                   /note="SLVS -> RKET (in Ref. 1; AAA30125)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   149 AA;  16619 MW;  C9A2AB8EEA9B9ECC CRC64;
     MVSHLKKTRK LRGHVSHGHG RVGKHRKGGC RGGRGKAGGM HHHRILMEKW HPGYYGKLGM
     RTFHLKKNPL HCPVVNIDKL WSLVSDATRQ KYAEDKKKVP VIDVTKAGFF KVLGKGRLPN
     QPVVVKAKYF SKTAERRIVA VGGACVLTA