AATC2_HUMAN
ID AATC2_HUMAN Reviewed; 421 AA.
AC Q8NHS2; A8MWL4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Putative aspartate aminotransferase, cytoplasmic 2;
DE EC=2.6.1.1;
DE AltName: Full=Glutamate oxaloacetate transaminase 1-like protein 1;
DE AltName: Full=Transaminase A-like protein 1;
GN Name=GOT1L1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + L-aspartate = L-glutamate + oxaloacetate;
CC Xref=Rhea:RHEA:21824, ChEBI:CHEBI:16452, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:29991; EC=2.6.1.1;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- MISCELLANEOUS: In eukaryotes there are cytoplasmic, mitochondrial and
CC chloroplastic isozymes.
CC -!- SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent
CC aminotransferase family. {ECO:0000305}.
CC -!- CAUTION: The residues that bind the substrate in other aspartate
CC aminotransferases are not conserved. {ECO:0000305}.
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DR EMBL; AC130304; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC029504; AAH29504.1; -; mRNA.
DR CCDS; CCDS47839.1; -.
DR RefSeq; NP_689626.2; NM_152413.2.
DR AlphaFoldDB; Q8NHS2; -.
DR SMR; Q8NHS2; -.
DR BioGRID; 126475; 5.
DR IntAct; Q8NHS2; 3.
DR STRING; 9606.ENSP00000303077; -.
DR BioMuta; GOT1L1; -.
DR DMDM; 269849534; -.
DR MassIVE; Q8NHS2; -.
DR PaxDb; Q8NHS2; -.
DR PeptideAtlas; Q8NHS2; -.
DR PRIDE; Q8NHS2; -.
DR Antibodypedia; 23475; 79 antibodies from 19 providers.
DR DNASU; 137362; -.
DR Ensembl; ENST00000307599.5; ENSP00000303077.4; ENSG00000169154.7.
DR GeneID; 137362; -.
DR KEGG; hsa:137362; -.
DR MANE-Select; ENST00000307599.5; ENSP00000303077.4; NM_152413.3; NP_689626.2.
DR UCSC; uc011lbj.2; human.
DR CTD; 137362; -.
DR GeneCards; GOT1L1; -.
DR HGNC; HGNC:28487; GOT1L1.
DR HPA; ENSG00000169154; Tissue enriched (testis).
DR neXtProt; NX_Q8NHS2; -.
DR OpenTargets; ENSG00000169154; -.
DR PharmGKB; PA142671724; -.
DR VEuPathDB; HostDB:ENSG00000169154; -.
DR eggNOG; KOG1412; Eukaryota.
DR GeneTree; ENSGT00950000183082; -.
DR HOGENOM; CLU_032440_1_2_1; -.
DR InParanoid; Q8NHS2; -.
DR OMA; LAYKVCM; -.
DR OrthoDB; 1104596at2759; -.
DR PhylomeDB; Q8NHS2; -.
DR TreeFam; TF314089; -.
DR BRENDA; 2.6.1.1; 2681.
DR PathwayCommons; Q8NHS2; -.
DR SignaLink; Q8NHS2; -.
DR BioGRID-ORCS; 137362; 11 hits in 1059 CRISPR screens.
DR GenomeRNAi; 137362; -.
DR Pharos; Q8NHS2; Tdark.
DR PRO; PR:Q8NHS2; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8NHS2; protein.
DR Bgee; ENSG00000169154; Expressed in left testis and 35 other tissues.
DR ExpressionAtlas; Q8NHS2; baseline and differential.
DR Genevisible; Q8NHS2; HS.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; IBA:GO_Central.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0006532; P:aspartate biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR InterPro; IPR004839; Aminotransferase_I/II.
DR InterPro; IPR000796; Asp_trans.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR PANTHER; PTHR11879; PTHR11879; 1.
DR Pfam; PF00155; Aminotran_1_2; 1.
DR PRINTS; PR00799; TRANSAMINASE.
DR SUPFAM; SSF53383; SSF53383; 1.
PE 2: Evidence at transcript level;
KW Aminotransferase; Cytoplasm; Pyridoxal phosphate; Reference proteome;
KW Transferase.
FT CHAIN 1..421
FT /note="Putative aspartate aminotransferase, cytoplasmic 2"
FT /id="PRO_0000332999"
FT MOD_RES 249
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
FT CONFLICT 223
FT /note="D -> G (in Ref. 2; AAH29504)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 47305 MW; 020AB902540E5B90 CRC64;
MPTLSVFMDV PLAHKLEGSL LKTYKQDDYP NKIFLAYRVC MTNEGHPWVS LVVQKTRLQI
SQDPSLNYEY LPTMGLKSFI QASLALLFGK HSQAIVENRV GGVHTVGDSG AFQLGVQFLR
AWHKDARIVY IISSQKELHG LVFQDMGFTV YEYSVWDPKK LCMDPDILLN VVEQIPHGCV
LVMGNIIDCK LTPSGWAKLM SMIKSKQIFP FFDIPCQGLY TSDLEEDTRI LQYFVSQGFE
FFCSQSLSKN FGIYDEGVGM LVVVAVNNQQ LLCVLSQLEG LAQALWLNPP NTGARVITSI
LCNPALLGEW KQSLKEVVEN IMLTKEKVKE KLQLLGTPGS WGHITEQSGT HGYLGLNSQQ
VEYLVRKKHI YIPKNGQINF SCINANNINY ITEGINEAVL LTESSEMCLP KEKKTLIGIK
L
