AATP5_ARATH
ID AATP5_ARATH Reviewed; 530 AA.
AC Q9LH84;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=AAA-ATPase At3g28510;
DE EC=3.6.1.- {ECO:0000250|UniProtKB:Q9FLD5};
GN OrderedLocusNames=At3g28510 {ECO:0000312|EMBL:AAM20543.1};
GN ORFNames=T20D4.2 {ECO:0000312|EMBL:BAB01953.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000250|UniProtKB:Q9FLD5};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q9FLD5};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. BCS1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AP002059; BAB01953.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77454.1; -; Genomic_DNA.
DR EMBL; AY099692; AAM20543.1; -; mRNA.
DR RefSeq; NP_189492.1; NM_113771.4.
DR AlphaFoldDB; Q9LH84; -.
DR STRING; 3702.AT3G28510.1; -.
DR SwissPalm; Q9LH84; -.
DR PaxDb; Q9LH84; -.
DR PRIDE; Q9LH84; -.
DR ProteomicsDB; 244339; -.
DR EnsemblPlants; AT3G28510.1; AT3G28510.1; AT3G28510.
DR GeneID; 822481; -.
DR Gramene; AT3G28510.1; AT3G28510.1; AT3G28510.
DR KEGG; ath:AT3G28510; -.
DR Araport; AT3G28510; -.
DR TAIR; locus:2098658; AT3G28510.
DR eggNOG; KOG0743; Eukaryota.
DR HOGENOM; CLU_010189_0_1_1; -.
DR InParanoid; Q9LH84; -.
DR OMA; YIRITFH; -.
DR OrthoDB; 532729at2759; -.
DR PhylomeDB; Q9LH84; -.
DR PRO; PR:Q9LH84; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LH84; baseline and differential.
DR Genevisible; Q9LH84; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR025753; AAA_N_dom.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF14363; AAA_assoc; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Hydrolase; Magnesium; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..530
FT /note="AAA-ATPase At3g28510"
FT /id="PRO_0000434707"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 312..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 463..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 463..510
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..530
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 250..257
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 530 AA; 61557 MW; 5C4D8FF105AA2F5B CRC64;
MLETGAIWGI TGTTVTSFMF FWAIYKQYVP AHFRAYVERY FHKMIGWISY YVDIKFTEYT
DEGLKRSQAY DSIRNYLASK STALAKRLKA NETKNSKSLV FSMDDHEEIE DEFEGVKVKW
YSNVKVIQPQ SNYGQRSSEE RRHFTLSFHR RHRGMIIETY LDHVLREGKA IGLMNRERKL
YTNNSSQEWY PWRSGKWSNV PFHHPATFET LAMDPEKKEG IKKDLIKFSK GKDYYKKVGK
PWKRGYLLFG PPGTGKSTMI AAIANFLDYD VYDLELTTVK DNSELKKLLL DTTSKSIIVI
EDIDCSLDLT GQRKKKKEED EEEDGEEKKE GEKKPKVDDK QSKVTLSGLL NSIDGLWSAC
SGEKIIVFTT NFVDKLDPAL IRRGRMDNHI EMSYCKFEAF KVLAKNYLEI ETHDLYGEIE
RKLEETDMSP ADVAETLMPK SDEEDADICI KRLVKTLEEE KEKARKLAEE EEKKKAEKEA
KKMKKAEEAE EKKKKTEEDE KKEKVKAKEE NGNVSQQNGN SIDLNKKSDS
