RL34_MOUSE
ID RL34_MOUSE Reviewed; 117 AA.
AC Q9D1R9; Q497V4;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=60S ribosomal protein L34;
GN Name=Rpl34;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=BALB/cJ, and C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, Mammary gland, and Thyroid;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-43, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Component of the large ribosomal subunit. The ribosome is a
CC large ribonucleoprotein complex responsible for the synthesis of
CC proteins in the cell. {ECO:0000250|UniProtKB:P49207}.
CC -!- SUBUNIT: Component of the large ribosomal subunit.
CC {ECO:0000250|UniProtKB:P49207}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P49207}. Cytoplasm
CC {ECO:0000250|UniProtKB:P49207}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q29223}. Note=Detected on cytosolic polysomes
CC (By similarity). Detected in ribosomes that are associated with the
CC rough endoplasmic reticulum (By similarity).
CC {ECO:0000250|UniProtKB:P49207, ECO:0000250|UniProtKB:Q29223}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL34 family.
CC {ECO:0000305}.
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DR EMBL; AK003177; BAB22621.1; -; mRNA.
DR EMBL; AK146252; BAE27014.1; -; mRNA.
DR EMBL; CH466532; EDL12210.1; -; Genomic_DNA.
DR EMBL; CH466532; EDL12212.1; -; Genomic_DNA.
DR EMBL; BC028517; AAH28517.1; -; mRNA.
DR EMBL; BC058118; AAH58118.1; -; mRNA.
DR EMBL; BC100360; AAI00361.1; -; mRNA.
DR CCDS; CCDS17841.1; -.
DR RefSeq; NP_001005859.1; NM_001005859.3.
DR RefSeq; NP_001186279.1; NM_001199350.1.
DR RefSeq; NP_001274510.1; NM_001287581.1.
DR RefSeq; NP_081000.1; NM_026724.2.
DR RefSeq; XP_001481222.1; XM_001481172.6.
DR PDB; 6SWA; EM; 3.10 A; e=1-117.
DR PDB; 7LS1; EM; 3.30 A; a2=1-117.
DR PDB; 7LS2; EM; 3.10 A; a2=1-117.
DR PDBsum; 6SWA; -.
DR PDBsum; 7LS1; -.
DR PDBsum; 7LS2; -.
DR AlphaFoldDB; Q9D1R9; -.
DR SMR; Q9D1R9; -.
DR BioGRID; 212853; 8.
DR BioGRID; 546909; 6.
DR BioGRID; 787025; 2.
DR ComplexPortal; CPX-5262; 60S cytosolic large ribosomal subunit.
DR IntAct; Q9D1R9; 5.
DR MINT; Q9D1R9; -.
DR STRING; 10090.ENSMUSP00000086614; -.
DR iPTMnet; Q9D1R9; -.
DR PhosphoSitePlus; Q9D1R9; -.
DR SwissPalm; Q9D1R9; -.
DR EPD; Q9D1R9; -.
DR jPOST; Q9D1R9; -.
DR MaxQB; Q9D1R9; -.
DR PaxDb; Q9D1R9; -.
DR PeptideAtlas; Q9D1R9; -.
DR PRIDE; Q9D1R9; -.
DR ProteomicsDB; 299821; -.
DR TopDownProteomics; Q9D1R9; -.
DR Antibodypedia; 26278; 154 antibodies from 23 providers.
DR DNASU; 68436; -.
DR Ensembl; ENSMUST00000062601; ENSMUSP00000086614; ENSMUSG00000062006.
DR Ensembl; ENSMUST00000079085; ENSMUSP00000078092; ENSMUSG00000062006.
DR Ensembl; ENSMUST00000196202; ENSMUSP00000142486; ENSMUSG00000062006.
DR GeneID; 619547; -.
DR GeneID; 68436; -.
DR KEGG; mmu:619547; -.
DR KEGG; mmu:68436; -.
DR UCSC; uc008rjd.2; mouse.
DR CTD; 6164; -.
DR CTD; 619547; -.
DR MGI; MGI:1915686; Rpl34.
DR VEuPathDB; HostDB:ENSMUSG00000062006; -.
DR eggNOG; KOG1790; Eukaryota.
DR GeneTree; ENSGT00390000008294; -.
DR HOGENOM; CLU_118652_0_1_1; -.
DR InParanoid; Q9D1R9; -.
DR OMA; CGNCVKD; -.
DR OrthoDB; 1517591at2759; -.
DR PhylomeDB; Q9D1R9; -.
DR TreeFam; TF314326; -.
DR Reactome; R-MMU-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-MMU-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-MMU-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-MMU-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-MMU-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-MMU-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR BioGRID-ORCS; 619547; 9 hits in 21 CRISPR screens.
DR BioGRID-ORCS; 68436; 24 hits in 50 CRISPR screens.
DR ChiTaRS; Rpl34; mouse.
DR PRO; PR:Q9D1R9; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9D1R9; protein.
DR Bgee; ENSMUSG00000062006; Expressed in yolk sac and 63 other tissues.
DR ExpressionAtlas; Q9D1R9; baseline and differential.
DR Genevisible; Q9D1R9; MM.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IPI:ComplexPortal.
DR GO; GO:0022626; C:cytosolic ribosome; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0003735; F:structural constituent of ribosome; ISO:MGI.
DR GO; GO:0002181; P:cytoplasmic translation; IC:ComplexPortal.
DR InterPro; IPR008195; Ribosomal_L34Ae.
DR InterPro; IPR018065; Ribosomal_L34e_CS.
DR Pfam; PF01199; Ribosomal_L34e; 1.
DR PRINTS; PR01250; RIBOSOMALL34.
DR PROSITE; PS01145; RIBOSOMAL_L34E; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytoplasm; Endoplasmic reticulum;
KW Isopeptide bond; Phosphoprotein; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; Ubl conjugation.
FT CHAIN 1..117
FT /note="60S ribosomal protein L34"
FT /id="PRO_0000131832"
FT MOD_RES 12
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P49207"
FT MOD_RES 36
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P49207"
FT MOD_RES 43
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT CROSSLNK 108
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P49207"
SQ SEQUENCE 117 AA; 13293 MW; 6775B00B98CB6A30 CRC64;
MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR
PKVLMRLSKT QKHVSRAYGG SMCAKCVRDR IKRAFLIEEQ KIVVKVLKAQ AQSQKAK
