ID   AAT_METEX               Reviewed;          93 AA.
AC   P52069;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Putative aspartate aminotransferase;
DE            Short=AspAT;
DE            EC=2.6.1.1;
DE   AltName: Full=ORF2;
DE   AltName: Full=Transaminase A;
DE   Flags: Fragment;
OS   Methylorubrum extorquens (Methylobacterium dichloromethanicum)
OS   (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IBT 6;
RX   PubMed=7763712; DOI=10.1007/bf00192084;
RA   Valentin H.E., Steinbuechel A.;
RT   "Cloning and characterization of the Methylobacterium extorquens
RT   polyhydroxyalkanoic-acid-synthase structural gene.";
RL   Appl. Microbiol. Biotechnol. 39:309-317(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxoglutarate + L-aspartate = L-glutamate + oxaloacetate;
CC         Xref=Rhea:RHEA:21824, ChEBI:CHEBI:16452, ChEBI:CHEBI:16810,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:29991; EC=2.6.1.1;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000305};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; L07893; AAA72329.1; -; Genomic_DNA.
DR   AlphaFoldDB; P52069; -.
DR   SMR; P52069; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   3: Inferred from homology;
KW   Aminotransferase; Cytoplasm; Pyridoxal phosphate; Transferase.
FT   CHAIN           1..>93
FT                   /note="Putative aspartate aminotransferase"
FT                   /id="PRO_0000123842"
FT   NON_TER         93
SQ   SEQUENCE   93 AA;  10424 MW;  201376961C632611 CRC64;
     MTDFHRIKRL PPYVFEQVNR IKAAARARGA DIIDLGMGNP DLDAPRHVIE KLVETAGKPR
     TDRYSASKGI AGLRRAQAGY YQRRFGVSLN PDT