RL3_DROME
ID RL3_DROME Reviewed; 416 AA.
AC O16797; Q0KI83; Q6NNE9; Q9VGR4; Q9VGR5; Q9VGR6;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=60S ribosomal protein L3;
GN Name=RpL3; ORFNames=CG4863;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RX PubMed=9661671; DOI=10.1016/s0378-1119(98)00145-0;
RA Chan H.Y.E., Zhang Y., Hoheisel J.D., O'Kane C.J.;
RT "Identification and characterization of the gene for Drosophila L3
RT ribosomal protein.";
RL Gene 212:119-125(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (6.0 ANGSTROMS) OF THE 80S RIBOSOME.
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- FUNCTION: The L3 protein is a component of the large subunit of
CC cytoplasmic ribosomes.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=A; Synonyms=H;
CC IsoId=O16797-1; Sequence=Displayed;
CC Name=G;
CC IsoId=O16797-5; Sequence=VSP_005714, VSP_005716;
CC Name=D;
CC IsoId=O16797-2; Sequence=VSP_005715, VSP_005717;
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL3 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR96131.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF016835; AAC26144.1; -; mRNA.
DR EMBL; AE014297; AAF54610.2; -; Genomic_DNA.
DR EMBL; AE014297; AAF54611.2; -; Genomic_DNA.
DR EMBL; AE014297; AAF54612.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13496.2; -; Genomic_DNA.
DR EMBL; BT011339; AAR96131.1; ALT_FRAME; mRNA.
DR RefSeq; NP_524316.1; NM_079592.3. [O16797-1]
DR RefSeq; NP_731547.1; NM_169377.2. [O16797-2]
DR RefSeq; NP_731548.2; NM_169378.2. [O16797-1]
DR RefSeq; NP_731550.2; NM_169380.2. [O16797-5]
DR PDB; 4V6W; EM; 6.00 A; CB=1-416.
DR PDB; 6XU6; EM; 3.50 A; CB=1-414.
DR PDB; 6XU7; EM; 4.90 A; CB=1-414.
DR PDB; 6XU8; EM; 3.00 A; CB=1-414.
DR PDBsum; 4V6W; -.
DR PDBsum; 6XU6; -.
DR PDBsum; 6XU7; -.
DR PDBsum; 6XU8; -.
DR AlphaFoldDB; O16797; -.
DR SMR; O16797; -.
DR BioGRID; 66481; 138.
DR DIP; DIP-17151N; -.
DR IntAct; O16797; 3.
DR MINT; O16797; -.
DR STRING; 7227.FBpp0081822; -.
DR PaxDb; O16797; -.
DR PeptideAtlas; O16797; -.
DR PRIDE; O16797; -.
DR DNASU; 41347; -.
DR EnsemblMetazoa; FBtr0082346; FBpp0081822; FBgn0020910. [O16797-1]
DR EnsemblMetazoa; FBtr0082347; FBpp0081823; FBgn0020910. [O16797-2]
DR EnsemblMetazoa; FBtr0302586; FBpp0291742; FBgn0020910. [O16797-5]
DR EnsemblMetazoa; FBtr0336783; FBpp0307759; FBgn0020910. [O16797-1]
DR GeneID; 41347; -.
DR KEGG; dme:Dmel_CG4863; -.
DR CTD; 6122; -.
DR FlyBase; FBgn0020910; RpL3.
DR VEuPathDB; VectorBase:FBgn0020910; -.
DR eggNOG; KOG0746; Eukaryota.
DR GeneTree; ENSGT00390000017606; -.
DR HOGENOM; CLU_033361_2_1_1; -.
DR InParanoid; O16797; -.
DR OMA; HQRTEYN; -.
DR PhylomeDB; O16797; -.
DR Reactome; R-DME-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-DME-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-DME-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-DME-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-DME-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR SignaLink; O16797; -.
DR BioGRID-ORCS; 41347; 3 hits in 3 CRISPR screens.
DR ChiTaRS; RpL3; fly.
DR GenomeRNAi; 41347; -.
DR PRO; PR:O16797; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0020910; Expressed in seminal fluid secreting gland and 43 other tissues.
DR ExpressionAtlas; O16797; baseline and differential.
DR Genevisible; O16797; DM.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0022626; C:cytosolic ribosome; IDA:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:FlyBase.
DR GO; GO:0002181; P:cytoplasmic translation; TAS:FlyBase.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR Gene3D; 4.10.960.10; -; 1.
DR InterPro; IPR045077; L3_arc_euk.
DR InterPro; IPR000597; Ribosomal_L3.
DR InterPro; IPR019926; Ribosomal_L3_CS.
DR InterPro; IPR044892; Ribosomal_L3_dom_3_arc_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR11363; PTHR11363; 1.
DR Pfam; PF00297; Ribosomal_L3; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR PROSITE; PS00474; RIBOSOMAL_L3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..416
FT /note="60S ribosomal protein L3"
FT /id="PRO_0000077237"
FT VAR_SEQ 122..138
FT /note="WYKSKKKAFTKASKKWT -> CSSISLLRELFKSLNVV (in isoform
FT G)"
FT /evidence="ECO:0000305"
FT /id="VSP_005714"
FT VAR_SEQ 124..161
FT /note="KSKKKAFTKASKKWTDDLGKKSIENDFRKMLRYCKVIR -> VSEDHVVVLP
FT TPFVAIFFAPGVHTHKMAADTCFLLESG (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_005715"
FT VAR_SEQ 139..416
FT /note="Missing (in isoform G)"
FT /evidence="ECO:0000305"
FT /id="VSP_005716"
FT VAR_SEQ 162..416
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_005717"
SQ SEQUENCE 416 AA; 46916 MW; 779D843982334AA5 CRC64;
MSHRKFSAPR HGSMAFYPKK RSARHRGKVK AFPKDDASKP VHLTCFIGYK AGMTHIVREA
DRPGSKINKK EVVEAVTVLE TPPMIVVGAV GYIETPFGLR ALVNVWAQHL SEECRRRFYK
NWYKSKKKAF TKASKKWTDD LGKKSIENDF RKMLRYCKVI RVIAHSQIRL IKQRQKKAHV
MEIQLNGGSI EDKVKWAREH LEKPIQVSNV FGQDEMIDCV GVTKGKGFKG VTSRWHTKKL
PRKTHKGLRK VACIGAWHPS RVSTTVARAG QKGYHHRTEI NKKIYRIGAG IHTKDGKVIK
NNASTEYDLT DKSITPMGGF PHYGEVNNDF VMIKGCCIGS KKRIITLRKS LLKHTKRSAL
EQIKLKFIDT SSKMGHGRFQ TPADKLAFMG PLKKDRLKEE AAATTAAAAA ATTTSA
