RL40_CANLF
ID RL40_CANLF Reviewed; 128 AA.
AC P63050; P63049; Q7JK34; Q9XSV1;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Ubiquitin-60S ribosomal protein L40;
DE AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1;
DE Contains:
DE RecName: Full=Ubiquitin;
DE Contains:
DE RecName: Full=60S ribosomal protein L40;
DE AltName: Full=CEP52;
DE Flags: Precursor;
GN Name=UBA52; Synonyms=UBCEP2; ORFNames=B9;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Cocker spaniel; TISSUE=Kidney;
RA Kano R.;
RT "Canis familiaris ubiquitin gene.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND SUBCELLULAR LOCATION.
RC TISSUE=Thyroid;
RX PubMed=10964405; DOI=10.1006/abio.2000.4674;
RA Pichon B., Mercan D., Pouillon V., Christophe-Hobertus C., Christophe D.;
RT "A method for the large-scale cloning of nuclear proteins and nuclear
RT targeting sequences on a functional basis.";
RL Anal. Biochem. 284:231-239(2000).
CC -!- FUNCTION: [Ubiquitin]: Exists either covalently attached to another
CC protein, or free (unanchored). When covalently bound, it is conjugated
CC to target proteins via an isopeptide bond either as a monomer
CC (monoubiquitin), a polymer linked via different Lys residues of the
CC ubiquitin (polyubiquitin chains) or a linear polymer linked via the
CC initiator Met of the ubiquitin (linear polyubiquitin chains).
CC Polyubiquitin chains, when attached to a target protein, have different
CC functions depending on the Lys residue of the ubiquitin that is linked:
CC Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved
CC in ERAD (endoplasmic reticulum-associated degradation) and in cell-
CC cycle regulation; Lys-29-linked is involved in proteotoxic stress
CC response and cell cycle; Lys-33-linked is involved in kinase
CC modification; Lys-48-linked is involved in protein degradation via the
CC proteasome; Lys-63-linked is involved in endocytosis, DNA-damage
CC responses as well as in signaling processes leading to activation of
CC the transcription factor NF-kappa-B. Linear polymer chains formed via
CC attachment by the initiator Met lead to cell signaling. Ubiquitin is
CC usually conjugated to Lys residues of target proteins, however, in rare
CC cases, conjugation to Cys or Ser residues has been observed. When
CC polyubiquitin is free (unanchored-polyubiquitin), it also has distinct
CC roles, such as in activation of protein kinases, and in signaling.
CC {ECO:0000250|UniProtKB:P62987}.
CC -!- FUNCTION: [60S ribosomal protein L40]: Component of the 60S subunit of
CC the ribosome. Ribosomal protein L40 is essential for translation of a
CC subset of cellular transcripts, and especially for cap-dependent
CC translation of vesicular stomatitis virus mRNAs.
CC {ECO:0000250|UniProtKB:P62987}.
CC -!- SUBUNIT: Ribosomal protein L40 is part of the 60S ribosomal subunit.
CC Interacts with UBQLN1 (via UBA domain). {ECO:0000250|UniProtKB:P62987}.
CC -!- SUBCELLULAR LOCATION: [Ubiquitin]: Cytoplasm {ECO:0000250}. Nucleus.
CC -!- SUBCELLULAR LOCATION: [60S ribosomal protein L40]: Cytoplasm
CC {ECO:0000250}.
CC -!- PTM: [Ubiquitin]: Phosphorylated at Ser-65 by PINK1 during mitophagy.
CC Phosphorylated ubiquitin specifically binds and activates parkin
CC (PRKN), triggering mitophagy. Phosphorylation does not affect E1-
CC mediated E2 charging of ubiquitin but affects discharging of E2 enzymes
CC to form polyubiquitin chains. It also affects deubiquitination by
CC deubiquitinase enzymes such as USP30. {ECO:0000250|UniProtKB:P62987}.
CC -!- PTM: [Ubiquitin]: Mono-ADP-ribosylated at the C-terminus by PARP9, a
CC component of the PPAR9-DTX3L complex. ADP-ribosylation requires
CC processing by E1 and E2 enzymes and prevents ubiquitin conjugation to
CC substrates such as histones. {ECO:0000250|UniProtKB:P62987}.
CC -!- MISCELLANEOUS: Ubiquitin is encoded by 4 different genes. Uba52 and
CC Rps27a genes code for a single copy of ubiquitin fused to the ribosomal
CC proteins L40 and S27a, respectively. UBB and UBC genes code for a
CC polyubiquitin precursor with exact head to tail repeats, the number of
CC repeats differ between species and strains.
CC -!- SIMILARITY: In the N-terminal section; belongs to the ubiquitin family.
CC {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the eukaryotic
CC ribosomal protein eL40 family. {ECO:0000305}.
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DR EMBL; AB032025; BAA83996.1; -; mRNA.
DR EMBL; AJ388512; CAB46814.1; -; mRNA.
DR RefSeq; NP_001121567.1; NM_001128095.2.
DR RefSeq; XP_005632363.1; XM_005632306.2.
DR RefSeq; XP_013977202.1; XM_014121727.1.
DR AlphaFoldDB; P63050; -.
DR SMR; P63050; -.
DR STRING; 9615.ENSCAFP00000021714; -.
DR PaxDb; P63050; -.
DR Ensembl; ENSCAFT00030029916; ENSCAFP00030026075; ENSCAFG00030016247.
DR Ensembl; ENSCAFT00040031071; ENSCAFP00040027010; ENSCAFG00040016838.
DR Ensembl; ENSCAFT00040042825; ENSCAFP00040037359; ENSCAFG00040023038.
DR Ensembl; ENSCAFT00845036939; ENSCAFP00845028922; ENSCAFG00845020932.
DR GeneID; 403722; -.
DR KEGG; cfa:403722; -.
DR CTD; 7311; -.
DR VEuPathDB; HostDB:ENSCAFG00845020932; -.
DR eggNOG; KOG0003; Eukaryota.
DR GeneTree; ENSGT00940000153593; -.
DR HOGENOM; CLU_010412_3_4_1; -.
DR InParanoid; P63050; -.
DR OMA; AREYNQN; -.
DR OrthoDB; 1536766at2759; -.
DR TreeFam; TF352129; -.
DR Reactome; R-CFA-110312; Translesion synthesis by REV1.
DR Reactome; R-CFA-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR Reactome; R-CFA-110320; Translesion Synthesis by POLH.
DR Reactome; R-CFA-1169091; Activation of NF-kappaB in B cells.
DR Reactome; R-CFA-1234176; Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
DR Reactome; R-CFA-1253288; Downregulation of ERBB4 signaling.
DR Reactome; R-CFA-1295596; Spry regulation of FGF signaling.
DR Reactome; R-CFA-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-CFA-168638; NOD1/2 Signaling Pathway.
DR Reactome; R-CFA-174048; APC/C:Cdc20 mediated degradation of Cyclin B.
DR Reactome; R-CFA-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR Reactome; R-CFA-174113; SCF-beta-TrCP mediated degradation of Emi1.
DR Reactome; R-CFA-174154; APC/C:Cdc20 mediated degradation of Securin.
DR Reactome; R-CFA-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-CFA-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR Reactome; R-CFA-179409; APC-Cdc20 mediated degradation of Nek2A.
DR Reactome; R-CFA-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-CFA-182971; EGFR downregulation.
DR Reactome; R-CFA-187577; SCF(Skp2)-mediated degradation of p27/p21.
DR Reactome; R-CFA-195253; Degradation of beta-catenin by the destruction complex.
DR Reactome; R-CFA-201681; TCF dependent signaling in response to WNT.
DR Reactome; R-CFA-202424; Downstream TCR signaling.
DR Reactome; R-CFA-205043; NRIF signals cell death from the nucleus.
DR Reactome; R-CFA-209543; p75NTR recruits signalling complexes.
DR Reactome; R-CFA-209560; NF-kB is activated and signals survival.
DR Reactome; R-CFA-2173788; Downregulation of TGF-beta receptor signaling.
DR Reactome; R-CFA-2173791; TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
DR Reactome; R-CFA-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR Reactome; R-CFA-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-CFA-2467813; Separation of Sister Chromatids.
DR Reactome; R-CFA-2559582; Senescence-Associated Secretory Phenotype (SASP).
DR Reactome; R-CFA-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-CFA-2672351; Stimuli-sensing channels.
DR Reactome; R-CFA-2871837; FCERI mediated NF-kB activation.
DR Reactome; R-CFA-3134975; Regulation of innate immune responses to cytosolic DNA.
DR Reactome; R-CFA-349425; Autodegradation of the E3 ubiquitin ligase COP1.
DR Reactome; R-CFA-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-CFA-382556; ABC-family proteins mediated transport.
DR Reactome; R-CFA-450302; activated TAK1 mediates p38 MAPK activation.
DR Reactome; R-CFA-450321; JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
DR Reactome; R-CFA-450408; AUF1 (hnRNP D0) binds and destabilizes mRNA.
DR Reactome; R-CFA-4608870; Asymmetric localization of PCP proteins.
DR Reactome; R-CFA-4641257; Degradation of AXIN.
DR Reactome; R-CFA-4641258; Degradation of DVL.
DR Reactome; R-CFA-4641263; Regulation of FZD by ubiquitination.
DR Reactome; R-CFA-5205685; PINK1-PRKN Mediated Mitophagy.
DR Reactome; R-CFA-532668; N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
DR Reactome; R-CFA-5357905; Regulation of TNFR1 signaling.
DR Reactome; R-CFA-5357956; TNFR1-induced NFkappaB signaling pathway.
DR Reactome; R-CFA-5358346; Hedgehog ligand biogenesis.
DR Reactome; R-CFA-5607761; Dectin-1 mediated noncanonical NF-kB signaling.
DR Reactome; R-CFA-5607764; CLEC7A (Dectin-1) signaling.
DR Reactome; R-CFA-5610780; Degradation of GLI1 by the proteasome.
DR Reactome; R-CFA-5610785; GLI3 is processed to GLI3R by the proteasome.
DR Reactome; R-CFA-5632684; Hedgehog 'on' state.
DR Reactome; R-CFA-5654726; Negative regulation of FGFR1 signaling.
DR Reactome; R-CFA-5654727; Negative regulation of FGFR2 signaling.
DR Reactome; R-CFA-5654732; Negative regulation of FGFR3 signaling.
DR Reactome; R-CFA-5654733; Negative regulation of FGFR4 signaling.
DR Reactome; R-CFA-5655862; Translesion synthesis by POLK.
DR Reactome; R-CFA-5656121; Translesion synthesis by POLI.
DR Reactome; R-CFA-5656169; Termination of translesion DNA synthesis.
DR Reactome; R-CFA-5658442; Regulation of RAS by GAPs.
DR Reactome; R-CFA-5668541; TNFR2 non-canonical NF-kB pathway.
DR Reactome; R-CFA-5675221; Negative regulation of MAPK pathway.
DR Reactome; R-CFA-5675482; Regulation of necroptotic cell death.
DR Reactome; R-CFA-5676590; NIK-->noncanonical NF-kB signaling.
DR Reactome; R-CFA-5685942; HDR through Homologous Recombination (HRR).
DR Reactome; R-CFA-5687128; MAPK6/MAPK4 signaling.
DR Reactome; R-CFA-5689603; UCH proteinases.
DR Reactome; R-CFA-5689877; Josephin domain DUBs.
DR Reactome; R-CFA-5689880; Ub-specific processing proteases.
DR Reactome; R-CFA-5689896; Ovarian tumor domain proteases.
DR Reactome; R-CFA-5689901; Metalloprotease DUBs.
DR Reactome; R-CFA-5693565; Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
DR Reactome; R-CFA-5696394; DNA Damage Recognition in GG-NER.
DR Reactome; R-CFA-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-CFA-5696397; Gap-filling DNA repair synthesis and ligation in GG-NER.
DR Reactome; R-CFA-5696400; Dual Incision in GG-NER.
DR Reactome; R-CFA-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-CFA-6782135; Dual incision in TC-NER.
DR Reactome; R-CFA-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-CFA-6783310; Fanconi Anemia Pathway.
DR Reactome; R-CFA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR Reactome; R-CFA-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-CFA-6804757; Regulation of TP53 Degradation.
DR Reactome; R-CFA-6804760; Regulation of TP53 Activity through Methylation.
DR Reactome; R-CFA-6807004; Negative regulation of MET activity.
DR Reactome; R-CFA-68867; Assembly of the pre-replicative complex.
DR Reactome; R-CFA-68949; Orc1 removal from chromatin.
DR Reactome; R-CFA-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR Reactome; R-CFA-69231; Cyclin D associated events in G1.
DR Reactome; R-CFA-69541; Stabilization of p53.
DR Reactome; R-CFA-69601; Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
DR Reactome; R-CFA-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-CFA-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-CFA-8849469; PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1.
DR Reactome; R-CFA-8854050; FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
DR Reactome; R-CFA-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-CFA-8856828; Clathrin-mediated endocytosis.
DR Reactome; R-CFA-8866427; VLDLR internalisation and degradation.
DR Reactome; R-CFA-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
DR Reactome; R-CFA-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-CFA-8939236; RUNX1 regulates transcription of genes involved in differentiation of HSCs.
DR Reactome; R-CFA-8939902; Regulation of RUNX2 expression and activity.
DR Reactome; R-CFA-8941858; Regulation of RUNX3 expression and activity.
DR Reactome; R-CFA-8948747; Regulation of PTEN localization.
DR Reactome; R-CFA-8948751; Regulation of PTEN stability and activity.
DR Reactome; R-CFA-8951664; Neddylation.
DR Reactome; R-CFA-901032; ER Quality Control Compartment (ERQC).
DR Reactome; R-CFA-9020702; Interleukin-1 signaling.
DR Reactome; R-CFA-9033241; Peroxisomal protein import.
DR Reactome; R-CFA-912631; Regulation of signaling by CBL.
DR Reactome; R-CFA-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR Reactome; R-CFA-917937; Iron uptake and transport.
DR Reactome; R-CFA-936440; Negative regulators of DDX58/IFIH1 signaling.
DR Reactome; R-CFA-937039; IRAK1 recruits IKK complex.
DR Reactome; R-CFA-937042; IRAK2 mediated activation of TAK1 complex.
DR Reactome; R-CFA-937072; TRAF6-mediated induction of TAK1 complex within TLR4 complex.
DR Reactome; R-CFA-9645460; Alpha-protein kinase 1 signaling pathway.
DR Reactome; R-CFA-9646399; Aggrephagy.
DR Reactome; R-CFA-9648002; RAS processing.
DR Reactome; R-CFA-9664873; Pexophagy.
DR Reactome; R-CFA-9705462; Inactivation of CSF3 (G-CSF) signaling.
DR Reactome; R-CFA-9706369; Negative regulation of FLT3.
DR Reactome; R-CFA-975144; IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
DR Reactome; R-CFA-975163; IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation.
DR Reactome; R-CFA-9755511; KEAP1-NFE2L2 pathway.
DR Reactome; R-CFA-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-CFA-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR Reactome; R-CFA-9762114; GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2.
DR Reactome; R-CFA-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR Proteomes; UP000002254; Chromosome 20.
DR Bgee; ENSCAFG00000018652; Expressed in cardiac muscle of left ventricle and 8 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IEA:Ensembl.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0031386; F:protein tag; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IBA:GO_Central.
DR GO; GO:0019941; P:modification-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 4.10.1060.50; -; 1.
DR InterPro; IPR038587; L40e_sf.
DR InterPro; IPR001975; Ribosomal_L40e.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR019954; Ubiquitin_CS.
DR InterPro; IPR019956; Ubiquitin_dom.
DR Pfam; PF01020; Ribosomal_L40e; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR PRINTS; PR00348; UBIQUITIN.
DR SMART; SM01377; Ribosomal_L40e; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS00299; UBIQUITIN_1; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 2: Evidence at transcript level;
KW ADP-ribosylation; Cytoplasm; Isopeptide bond; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Ubl conjugation.
FT CHAIN 1..76
FT /note="Ubiquitin"
FT /id="PRO_0000114794"
FT CHAIN 77..128
FT /note="60S ribosomal protein L40"
FT /id="PRO_0000138749"
FT DOMAIN 1..76
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT SITE 54
FT /note="Interacts with activating enzyme"
FT SITE 68
FT /note="Essential for function"
FT SITE 72
FT /note="Interacts with activating enzyme"
FT MOD_RES 65
FT /note="Phosphoserine; by PINK1"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT MOD_RES 76
FT /note="ADP-ribosylglycine"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT MOD_RES 98
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:P62986"
FT CROSSLNK 6
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 11
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 27
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 29
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 33
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 48
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 63
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P62987"
FT CROSSLNK 76
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-? in acceptor proteins)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
SQ SEQUENCE 128 AA; 14728 MW; 7BCB602ABEFAD02A CRC64;
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN
IQKESTLHLV LRLRGGIIEP SLRQLAQKYN CDKMICRKCY ARLHPRAVNC RKKKCGHTNN
LRPKKKVK
