RL4_DROME
ID RL4_DROME Reviewed; 401 AA.
AC P09180; Q9I7H6;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=60S ribosomal protein L4;
DE AltName: Full=L1;
GN Name=RpL4; Synonyms=RpL1; ORFNames=CG5502;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R;
RX PubMed=2492096; DOI=10.1093/nar/17.1.456;
RA Rafti F., Gargiulo G., Manzi A., Malva C., Graziani F.;
RT "Sequence of the ribosomal protein cDNA of D. melanogaster homologous to
RT the L1 ribosomal protein gene of X. laevis.";
RL Nucleic Acids Res. 17:456-456(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 63-186.
RC STRAIN=Oregon-R;
RX PubMed=3133637; DOI=10.1093/nar/16.11.4915;
RA Rafti F., Gargiulo G., Manzi A., Malva C., Grossi G., Andone S.,
RA Graziani F.;
RT "Isolation and structural analysis of a ribosomal protein gene in
RT D.melanogaster.";
RL Nucleic Acids Res. 16:4915-4926(1988).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (6.0 ANGSTROMS) OF THE 80S RIBOSOME.
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC {ECO:0000305}.
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DR EMBL; X13382; CAA31759.1; -; mRNA.
DR EMBL; AE014297; AAG22173.1; -; Genomic_DNA.
DR EMBL; AY069485; AAL39630.1; -; mRNA.
DR EMBL; X06881; CAA29998.1; -; Genomic_DNA.
DR PIR; S02209; R5FFL1.
DR RefSeq; NP_524538.2; NM_079814.3.
DR PDB; 4V6W; EM; 6.00 A; CC=1-401.
DR PDB; 6XU6; EM; 3.50 A; CC=2-393.
DR PDB; 6XU7; EM; 4.90 A; CC=2-393.
DR PDB; 6XU8; EM; 3.00 A; CC=2-393.
DR PDBsum; 4V6W; -.
DR PDBsum; 6XU6; -.
DR PDBsum; 6XU7; -.
DR PDBsum; 6XU8; -.
DR AlphaFoldDB; P09180; -.
DR SMR; P09180; -.
DR BioGRID; 68230; 113.
DR IntAct; P09180; 2.
DR MINT; P09180; -.
DR STRING; 7227.FBpp0084617; -.
DR PaxDb; P09180; -.
DR PRIDE; P09180; -.
DR DNASU; 43349; -.
DR EnsemblMetazoa; FBtr0085248; FBpp0084617; FBgn0003279.
DR GeneID; 43349; -.
DR KEGG; dme:Dmel_CG5502; -.
DR CTD; 6124; -.
DR FlyBase; FBgn0003279; RpL4.
DR VEuPathDB; VectorBase:FBgn0003279; -.
DR eggNOG; KOG1475; Eukaryota.
DR HOGENOM; CLU_026535_4_0_1; -.
DR InParanoid; P09180; -.
DR OMA; WHQKVNV; -.
DR OrthoDB; 878848at2759; -.
DR PhylomeDB; P09180; -.
DR Reactome; R-DME-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR Reactome; R-DME-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR Reactome; R-DME-72689; Formation of a pool of free 40S subunits.
DR Reactome; R-DME-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR Reactome; R-DME-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR Reactome; R-DME-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR SignaLink; P09180; -.
DR BioGRID-ORCS; 43349; 1 hit in 1 CRISPR screen.
DR ChiTaRS; RpL4; fly.
DR GenomeRNAi; 43349; -.
DR PRO; PR:P09180; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003279; Expressed in seminal fluid secreting gland and 12 other tissues.
DR ExpressionAtlas; P09180; baseline and differential.
DR Genevisible; P09180; DM.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0022626; C:cytosolic ribosome; IDA:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:FlyBase.
DR GO; GO:0002181; P:cytoplasmic translation; TAS:FlyBase.
DR Gene3D; 3.40.1370.10; -; 1.
DR InterPro; IPR025755; Ribos_L4_C_dom.
DR InterPro; IPR002136; Ribosomal_L4/L1e.
DR InterPro; IPR013000; Ribosomal_L4/L1e_euk/arc_CS.
DR InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR InterPro; IPR045240; Ribosomal_L4_euk/arch.
DR PANTHER; PTHR19431; PTHR19431; 1.
DR Pfam; PF14374; Ribos_L4_asso_C; 1.
DR Pfam; PF00573; Ribosomal_L4; 1.
DR SUPFAM; SSF52166; SSF52166; 1.
DR PROSITE; PS00939; RIBOSOMAL_L1E; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..401
FT /note="60S ribosomal protein L4"
FT /id="PRO_0000129359"
FT CONFLICT 117
FT /note="K -> R (in Ref. 5; CAA29998)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="L -> V (in Ref. 1; CAA31759)"
FT /evidence="ECO:0000305"
FT CONFLICT 378..401
FT /note="AANRAKLLAARKKKVAAKKPAAKK -> RPIAPSSGRPQEEGRRQEASGQEV
FT NLLSPE (in Ref. 1; CAA31759)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 401 AA; 45026 MW; C8B7EF9F732EF497 CRC64;
MSLGNARPLV SVYTEKNEPA KDKNICLPAV FKAPIRPDVV NEVHQLLRRN NRQAYAVSEL
AGHQTSAESW GTGRAVARIP RVRGGGTHRS GQGAFGNMCR GGRMFAPTKT FRRWHRKVNV
NQRRYALVSA IAASGVPALV QSKGHVIDGV SEFPLVVSDE VQKVQKTKQA VIFLRRLKIW
ADIQKVYKSQ RFRAGRGTMR DRRRIARRGP LVVYDKDEGL RKAFRNIPGI ETINVDKLNL
LKLAPGGHVG RFVIWTESAF ARLNDLFGTW KKPSTLKKGY NLPQPKMANT DLSRLLKSEE
IRKVLRDPRK RVFRSVRRLN PLTNVRQLIK LNPYAEVLKR RAALAAEKRT VAKVLAKAKK
QNVELAKSHF ANVATKAAAN RAKLLAARKK KVAAKKPAAK K
