ID   RL4_GEOSE               Reviewed;         207 AA.
AC   P28601;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=50S ribosomal protein L4;
DE            Short=BL4;
GN   Name=rplD;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=799;
RX   PubMed=1499563; DOI=10.1111/j.1432-1033.1992.tb17119.x;
RA   Herwig S., Kruft V., Wittmann-Liebold B.;
RT   "Primary structures of ribosomal proteins L3 and L4 from Bacillus
RT   stearothermophilus.";
RL   Eur. J. Biochem. 207:877-885(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 53-64 AND 66-75, AND CROSS-LINKING TO RRNA.
RC   STRAIN=799;
RX   PubMed=7556101; DOI=10.1002/j.1460-2075.1995.tb00137.x;
RA   Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.;
RT   "Protein-rRNA binding features and their structural and functional
RT   implications in ribosomes as determined by cross-linking studies.";
RL   EMBO J. 14:4578-4588(1995).
RN   [3]
RP   ISOLATION OF ANTIBIOTIC RESISTANT STRAINS.
RC   STRAIN=799;
RX   PubMed=2254291; DOI=10.1128/jb.172.12.7306-7309.1990;
RA   Schnier J., Gewitz H.S., Behrens S.E., Lee A., Ginther C., Leighton T.;
RT   "Isolation and characterization of Bacillus stearothermophilus 30S and 50S
RT   ribosomal protein mutations.";
RL   J. Bacteriol. 172:7306-7309(1990).
RN   [4]
RP   ABILITY TO REPRESS THE E.COLI S10 OPERON.
RX   PubMed=8722027; DOI=10.1139/o95-119;
RA   Zengel J.M., Vorozheikina D., Li X., Lindahl L.;
RT   "Regulation of the Escherichia coli S10 ribosomal protein operon by
RT   heterologous L4 ribosomal proteins.";
RL   Biochem. Cell Biol. 73:1105-1112(1995).
CC   -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC       initially binds near the 5'-end of the 23S rRNA. It is important during
CC       the early stages of 50S assembly. It makes multiple contacts with
CC       different domains of the 23S rRNA in the assembled 50S subunit and
CC       ribosome (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: This protein when expressed in E.coli represses the
CC       endogenous S10 operon; this may not occur in B.stearothermophilus
CC       however.
CC   -!- FUNCTION: Forms part of the polypeptide exit tunnel. {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC   -!- MISCELLANEOUS: Erythromycin, niddamycin, oleandomycin and spiramycin-
CC       resistant strains have changes in this protein as seen by gel
CC       electrophoresis; the nature of these changes has not been determined.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC       {ECO:0000305}.
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DR   EMBL; X67014; CAA47403.1; -; Genomic_DNA.
DR   PIR; S24364; S24364.
DR   RefSeq; WP_033008661.1; NZ_RCTK01000011.1.
DR   AlphaFoldDB; P28601; -.
DR   SMR; P28601; -.
DR   GeneID; 58573163; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1370.10; -; 1.
DR   HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR   InterPro; IPR002136; Ribosomal_L4/L1e.
DR   InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR   InterPro; IPR013005; Ribosomal_uL4/L1e.
DR   PANTHER; PTHR10746; PTHR10746; 1.
DR   Pfam; PF00573; Ribosomal_L4; 1.
DR   SUPFAM; SSF52166; SSF52166; 1.
DR   TIGRFAMs; TIGR03953; rplD_bact; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Direct protein sequencing; Ribonucleoprotein;
KW   Ribosomal protein; RNA-binding; rRNA-binding.
FT   CHAIN           1..207
FT                   /note="50S ribosomal protein L4"
FT                   /id="PRO_0000129182"
FT   REGION          44..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   207 AA;  22810 MW;  4ED00DDFC493A376 CRC64;
     MPKVALYNQN GQTVGEIELN DAVFGIEPNK HVLFEAVIMQ RASMRQGTHK TKNRAEVSGG
     GRKPWRQKGT GRARQGSIRA PQWRGGGTVF GPVPRSYSYK LPKKVRRLAI KSALSSKVLE
     NDIVVLDQLS LEAPKTKEMV KILNNLSVDR KALIVTDELN ENVYLSARNI PGVKVVPANG
     INVLDVLNHD KLVITKAAVE KVEEVLA