RL4_HAEIN
ID RL4_HAEIN Reviewed; 200 AA.
AC P44345;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=50S ribosomal protein L4;
GN Name=rplD; Synonyms=rpl4; OrderedLocusNames=HI_0778;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ABILITY TO REPRESS THE E.COLI S10
RP OPERON.
RX PubMed=8722027; DOI=10.1139/o95-119;
RA Zengel J.M., Vorozheikina D., Li X., Lindahl L.;
RT "Regulation of the Escherichia coli S10 ribosomal protein operon by
RT heterologous L4 ribosomal proteins.";
RL Biochem. Cell Biol. 73:1105-1112(1995).
RN [3]
RP EVIDENCE FOR REGULATION OF THE S10 OPERON.
RX PubMed=10498727; DOI=10.1128/jb.181.19.6124-6132.1999;
RA Allen T., Shen P., Samsel L., Liu R., Lindahl L., Zengel J.M.;
RT "Phylogenetic analysis of L4-mediated autogenous control of the S10
RT ribosomal protein operon.";
RL J. Bacteriol. 181:6124-6132(1999).
RN [4]
RP MACROLIDE RESISTANT VARIANTS.
RX PubMed=12604536; DOI=10.1128/aac.47.3.1017-1022.2003;
RA Peric M., Bozdogan B., Jacobs M.R., Appelbaum P.C.;
RT "Effects of an efflux mechanism and ribosomal mutations on macrolide
RT susceptibility of Haemophilus influenzae clinical isolates.";
RL Antimicrob. Agents Chemother. 47:1017-1022(2003).
CC -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC initially binds near the 5'-end of the 23S rRNA. It is important during
CC the early stages of 50S assembly. It makes multiple contacts with
CC different domains of the 23S rRNA in the assembled 50S subunit and
CC ribosome (By similarity). {ECO:0000250}.
CC -!- FUNCTION: Protein L4 is a both a transcriptional repressor and a
CC translational repressor protein. It regulates transcription of the S10
CC operon (to which L4 belongs) by causing premature termination of
CC transcription within the S10 leader. L4 controls the translation of the
CC S10 operon by binding to its mRNA (By similarity). {ECO:0000250}.
CC -!- FUNCTION: This protein when expressed in E.coli represses both
CC transcription and translation of the endogenous S10 operon. As the
CC H.influenzae S10 leader can be regulated in vitro by the E.coli L4
CC protein this strongly suggests the endogenous protein controls its own
CC S10 operon in a similar fashion.
CC -!- FUNCTION: Forms part of the polypeptide exit tunnel. {ECO:0000250}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22437.1; -; Genomic_DNA.
DR EMBL; U37797; AAB41511.1; -; Genomic_DNA.
DR PIR; F64092; F64092.
DR RefSeq; NP_438937.1; NC_000907.1.
DR RefSeq; WP_005652369.1; NC_000907.1.
DR AlphaFoldDB; P44345; -.
DR SMR; P44345; -.
DR STRING; 71421.HI_0778; -.
DR DrugBank; DB01321; Josamycin.
DR EnsemblBacteria; AAC22437; AAC22437; HI_0778.
DR KEGG; hin:HI_0778; -.
DR PATRIC; fig|71421.8.peg.817; -.
DR eggNOG; COG0088; Bacteria.
DR HOGENOM; CLU_041575_5_2_6; -.
DR OMA; PQVHILE; -.
DR PhylomeDB; P44345; -.
DR BioCyc; HINF71421:G1GJ1-818-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1370.10; -; 1.
DR HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR InterPro; IPR002136; Ribosomal_L4/L1e.
DR InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR InterPro; IPR013005; Ribosomal_uL4/L1e.
DR PANTHER; PTHR10746; PTHR10746; 1.
DR Pfam; PF00573; Ribosomal_L4; 1.
DR SUPFAM; SSF52166; SSF52166; 1.
DR TIGRFAMs; TIGR03953; rplD_bact; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Reference proteome; Repressor; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; Transcription;
KW Transcription regulation; Transcription termination;
KW Translation regulation.
FT CHAIN 1..200
FT /note="50S ribosomal protein L4"
FT /id="PRO_0000129223"
FT REGION 43..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 63
FT /note="G -> GGT (in strain: S4; confers macrolide
FT resistance)"
FT VARIANT 64
FT /note="T -> K (in strain: S39; confers macrolide
FT resistance)"
FT VARIANT 65
FT /note="G -> D (in strain: S27 and S47; confers macrolide
FT resistance)"
FT VARIANT 69
FT /note="A -> S (in strain: S50; confers macrolide
FT resistance)"
FT VARIANT 82
FT /note="T -> I (in strain: S54; confers macrolide
FT resistance)"
FT CONFLICT 65
FT /note="G -> A (in Ref. 2; AAB41511)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 200 AA; 21954 MW; A135A4762FE50927 CRC64;
MELQVVGANA LTVSETTFGR EFNEALIHQV VVAYAAGARQ GTRAQKTRAE VSGSGKKPWR
QKGTGRARAG DIKSPIWRSG GTTFAAKPQD HSQKVNKKMY RGAIKSILSE LVRQDRLVVV
EKFELDAPKT KVLVQKLKDL AVEDALIITA SLDENLFLAA RNLYKVDVRD VQGIDPVSLI
AFDKVIVTVD AVKQIEEILA
