RL4_MYCPN
ID RL4_MYCPN Reviewed; 212 AA.
AC P75579;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=50S ribosomal protein L4 {ECO:0000255|HAMAP-Rule:MF_01328};
GN Name=rplD {ECO:0000255|HAMAP-Rule:MF_01328}; OrderedLocusNames=MPN_166;
GN ORFNames=MP665;
OS Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS pneumoniae).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
RN [2]
RP ANTIBIOTIC RESISTANT VARIANTS.
RC STRAIN=ATCC 29342 / M129;
RX PubMed=14742195; DOI=10.1128/aac.48.2.460-465.2004;
RA Pereyre S., Guyot C., Renaudin H., Charron A., Bebear C., Bebear C.M.;
RT "In vitro selection and characterization of resistance to macrolides and
RT related antibiotics in Mycoplasma pneumoniae.";
RL Antimicrob. Agents Chemother. 48:460-465(2004).
CC -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC initially binds near the 5'-end of the 23S rRNA. It is important during
CC the early stages of 50S assembly. It makes multiple contacts with
CC different domains of the 23S rRNA in the assembled 50S subunit and
CC ribosome. {ECO:0000255|HAMAP-Rule:MF_01328}.
CC -!- FUNCTION: Forms part of the polypeptide exit tunnel.
CC {ECO:0000255|HAMAP-Rule:MF_01328}.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit. {ECO:0000255|HAMAP-
CC Rule:MF_01328}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC {ECO:0000255|HAMAP-Rule:MF_01328}.
CC -!- CAUTION: The antibiotic variants could harbor other changes as whole
CC genes were not sequenced. {ECO:0000305}.
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DR EMBL; U00089; AAB96313.1; -; Genomic_DNA.
DR PIR; S73991; S73991.
DR RefSeq; NP_109854.1; NC_000912.1.
DR RefSeq; WP_010874523.1; NC_000912.1.
DR AlphaFoldDB; P75579; -.
DR SMR; P75579; -.
DR IntAct; P75579; 4.
DR STRING; 272634.MPN_166; -.
DR EnsemblBacteria; AAB96313; AAB96313; MPN_166.
DR KEGG; mpn:MPN_166; -.
DR PATRIC; fig|272634.6.peg.184; -.
DR HOGENOM; CLU_041575_5_2_14; -.
DR OMA; PQVHILE; -.
DR BioCyc; MPNE272634:G1GJ3-276-MON; -.
DR Proteomes; UP000000808; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1370.10; -; 1.
DR HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR InterPro; IPR002136; Ribosomal_L4/L1e.
DR InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR InterPro; IPR013005; Ribosomal_uL4/L1e.
DR PANTHER; PTHR10746; PTHR10746; 1.
DR Pfam; PF00573; Ribosomal_L4; 1.
DR SUPFAM; SSF52166; SSF52166; 1.
DR TIGRFAMs; TIGR03953; rplD_bact; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding.
FT CHAIN 1..212
FT /note="50S ribosomal protein L4"
FT /id="PRO_0000129244"
FT REGION 55..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 60
FT /note="G -> GG (after 20 antibiotic passages; confers
FT resistance to various antibiotics)"
FT VARIANT 60
FT /note="G -> GGG (also to GGGG, after 47 antibiotic
FT passages; confers greater resistance than the above
FT variant)"
FT VARIANT 70
FT /note="H -> L (after 32, 37 and 48 antibiotic passages;
FT confers resistance to various antibiotics in combination
FT with a 23S rRNA mutation and up to 3 mutations in protein
FT L22)"
FT VARIANT 70
FT /note="H -> R (after 30 clindamycin passages; confers
FT resistance to clindamycin and josamycin)"
SQ SEQUENCE 212 AA; 23590 MW; 58421C03A2754D0B CRC64;
MAKLKLIKID GSFETEPVKL SPGLIAKELK QQPVFDAVLV EQASWRQGTH SILTKGEVRG
GGKKPYKQKH TGKARQGSTR NPHFVGGGIV FGPKPNRNYS LKLNKKAHTA ALHTVWSEKL
ASDNTHLVDQ NLFNKTEGKT KVMMQFLKSA KLLDKNVLFV VNTLNTNLEQ STSNIKNVQV
KHLDKVSVRD LMLANALLVE KEVLKALEGK FK
