ID   RL4_STRPN               Reviewed;         207 AA.
AC   Q97SV4;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=50S ribosomal protein L4;
GN   Name=rplD; OrderedLocusNames=SP_0210;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
RN   [2]
RP   VARIANT MACROLIDE RESISTANT 4AZ AND 5AZ.
RC   STRAIN=4Az, and 5Az;
RX   PubMed=10898684; DOI=10.1128/aac.44.8.2118-2125.2000;
RA   Tait-Kamradt A., Davies T., Cronan M., Jacobs M.R., Appelbaum P.C.,
RA   Sutcliffe J.;
RT   "Mutations in 23S rRNA and ribosomal protein L4 account for resistance in
RT   pneumococcal strains selected in vitro by macrolide passage.";
RL   Antimicrob. Agents Chemother. 44:2118-2125(2000).
RN   [3]
RP   VARIANTS MACROLIDE AND PENICILLIN G RESISTANT.
RC   STRAIN=16 East European strains, and Canadian strain BM4418;
RX   PubMed=11083646; DOI=10.1128/aac.44.12.3395-3401.2000;
RA   Tait-Kamradt A., Davies T., Appelbaum P.C., Depardieu F., Courvalin P.,
RA   Petitpas J., Wondrack L., Walker A., Jacobs M.R., Sutcliffe J.;
RT   "Two new mechanisms of macrolide resistance in clinical strains of
RT   Streptococcus pneumoniae from Eastern Europe and North America.";
RL   Antimicrob. Agents Chemother. 44:3395-3401(2000).
CC   -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC       initially binds near the 5'-end of the 23S rRNA. It is important during
CC       the early stages of 50S assembly. It makes multiple contacts with
CC       different domains of the 23S rRNA in the assembled 50S subunit and
CC       ribosome (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Forms part of the polypeptide exit tunnel. {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC       {ECO:0000305}.
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DR   EMBL; AE005672; AAK74390.1; -; Genomic_DNA.
DR   PIR; E95024; E95024.
DR   PIR; E97895; E97895.
DR   RefSeq; WP_000024543.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97SV4; -.
DR   SMR; Q97SV4; -.
DR   STRING; 170187.SP_0210; -.
DR   EnsemblBacteria; AAK74390; AAK74390; SP_0210.
DR   GeneID; 60233614; -.
DR   GeneID; 66805417; -.
DR   KEGG; spn:SP_0210; -.
DR   eggNOG; COG0088; Bacteria.
DR   OMA; PQVHILE; -.
DR   PhylomeDB; Q97SV4; -.
DR   BioCyc; SPNE170187:G1FZB-215-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1370.10; -; 1.
DR   HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR   InterPro; IPR002136; Ribosomal_L4/L1e.
DR   InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR   InterPro; IPR013005; Ribosomal_uL4/L1e.
DR   PANTHER; PTHR10746; PTHR10746; 1.
DR   Pfam; PF00573; Ribosomal_L4; 1.
DR   SUPFAM; SSF52166; SSF52166; 1.
DR   TIGRFAMs; TIGR03953; rplD_bact; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding.
FT   CHAIN           1..207
FT                   /note="50S ribosomal protein L4"
FT                   /id="PRO_0000129288"
FT   REGION          49..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         67
FT                   /note="Q -> QSQ (in strain: 5Az; confers macrolide
FT                   resistance. Grows slowly at 35 degrees Celsius and dies at
FT                   25 degrees Celsius)"
FT   VARIANT         69..71
FT                   /note="GTG -> TPS (in 12 Bulgarian and 4 Slovakian strains;
FT                   confers high resistance to erythromycin, azithromycin and
FT                   josamycin and lower resistance to clarithromycin and
FT                   penicillin G)"
FT   VARIANT         69
FT                   /note="G -> C (in strain: 4Az; confers macrolide
FT                   resistance)"
FT   VARIANT         71
FT                   /note="G -> GRQKGTG (in strain: BM4418; confers moderate
FT                   resistance to macrolides and high resistance to the
FT                   ketolide telithromycin. Grows slowly)"
SQ   SEQUENCE   207 AA;  22109 MW;  37C4141BCF588B57 CRC64;
     MANVTLFDQT GKEAGQVVLS DAVFGIEPNE SVVFDVIISQ RASLRQGTHA VKNRSAVSGG
     GRKPWRQKGT GRARQGSIRS PQWRGGGVVF GPTPRSYGYK LPQKVRRLAL KSVYSEKVAE
     NKFVAVDALS FTAPKTAEFA KVLAALSIDS KVLVILEEGN EFAALSARNL PNVKVATATT
     ASVLDIANSD KLLVTQAAIS KIEEVLA