ID   RL4_STRPY               Reviewed;         207 AA.
AC   P0C0D5; P60830; Q9A1X3;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=50S ribosomal protein L4;
GN   Name=rplD;
OS   Streptococcus pyogenes.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ni4277;
RX   PubMed=15128458; DOI=10.1186/1476-0711-3-5;
RA   Jalava J.P., Vaara M., Huovinen P.;
RT   "Mutation at the position 2058 of the 23S rRNA as a cause of macrolide
RT   resistance in Streptococcus pyogenes.";
RL   Ann. Clin. Microbiol. Antimicrob. 3:5-5(2004).
RN   [2]
RP   MACROLIDE ANTIBIOTIC RESISTANT VARIANT (STRAIN R791).
RC   STRAIN=r791 / Serotype 19A;
RX   PubMed=11850244; DOI=10.1128/aac.46.3.654-658.2002;
RA   Pihlajamaeki M., Kataja J., Seppaelae H., Elliot J., Leinonen M.,
RA   Huovinen P., Jalava J.;
RT   "Ribosomal mutations in Streptococcus pneumoniae clinical isolates.";
RL   Antimicrob. Agents Chemother. 46:654-658(2002).
RN   [3]
RP   VARIANT MACROLIDE RESISTANT.
RC   STRAIN=237;
RX   PubMed=12039885; DOI=10.1093/jac/dkf038;
RA   Malbruny B., Nagai K., Coquemont M., Bozdogan B., Andrasevic A.T.,
RA   Hupkova H., Leclercq R., Appelbaum P.C.;
RT   "Resistance to macrolides in clinical isolates of Streptococcus pyogenes
RT   due to ribosomal mutations.";
RL   J. Antimicrob. Chemother. 49:935-939(2002).
RN   [4]
RP   VARIANTS MACROLIDE RESISTANT.
RC   STRAIN=11, 124, and 390;
RX   PubMed=11959545; DOI=10.1128/aac.46.5.1199-1203.2002;
RA   Bingen E., Leclercq R., Fitoussi F., Brahimi N., Malbruny B., Deforche D.,
RA   Cohen R.;
RT   "Emergence of group A streptococcus strains with different mechanisms of
RT   macrolide resistance.";
RL   Antimicrob. Agents Chemother. 46:1199-1203(2002).
RN   [5]
RP   VARIANT MACROLIDE RESISTANT 65-TRP-ARG-66 DEL.
RX   PubMed=14616688; DOI=10.1046/j.1469-0691.2003.00670.x;
RA   Bozdogan B., Appelbaum P.C., Ednie L., Grivea I.N., Syrogiannopoulos G.A.;
RT   "Development of macrolide resistance by ribosomal protein L4 mutation in
RT   Streptococcus pyogenes during miocamycin treatment of an eight-year-old
RT   Greek child with tonsillopharyngitis.";
RL   Clin. Microbiol. Infect. 9:966-969(2003).
CC   -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC       initially binds near the 5'-end of the 23S rRNA. It is important during
CC       the early stages of 50S assembly. It makes multiple contacts with
CC       different domains of the 23S rRNA in the assembled 50S subunit and
CC       ribosome (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: Forms part of the polypeptide exit tunnel. {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ544683; CAD67589.1; -; Genomic_DNA.
DR   RefSeq; WP_002986657.1; NZ_WXZK01000002.1.
DR   AlphaFoldDB; P0C0D5; -.
DR   SMR; P0C0D5; -.
DR   GeneID; 66915996; -.
DR   eggNOG; COG0088; Bacteria.
DR   OMA; PQVHILE; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1370.10; -; 1.
DR   HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR   InterPro; IPR002136; Ribosomal_L4/L1e.
DR   InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR   InterPro; IPR013005; Ribosomal_uL4/L1e.
DR   PANTHER; PTHR10746; PTHR10746; 1.
DR   Pfam; PF00573; Ribosomal_L4; 1.
DR   SUPFAM; SSF52166; SSF52166; 1.
DR   TIGRFAMs; TIGR03953; rplD_bact; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Ribonucleoprotein; Ribosomal protein.
FT   CHAIN           1..207
FT                   /note="50S ribosomal protein L4"
FT                   /id="PRO_0000129290"
FT   REGION          49..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         64..65
FT                   /note="Missing (in strain: 11; confers resistance to
FT                   erythromycin and azithromycin)"
FT   VARIANT         65..66
FT                   /note="Missing (confers resistance to azithromycin,
FT                   erythromycin and clarithromycin)"
FT                   /evidence="ECO:0000269|PubMed:14616688"
FT   VARIANT         69..71
FT                   /note="GTG -> TPS (in strain: r791; highly resistant to
FT                   azithromycin, erythromycin and clarithromycin, less so to
FT                   spiramycin)"
FT   VARIANT         69..70
FT                   /note="Missing (in strain: 390; confers resistance to
FT                   erythromycin and azithromycin)"
FT   VARIANT         69
FT                   /note="G -> GKG (in strain: 237; resistant to azithromycin,
FT                   erythromycin and spiramycin)"
FT   VARIANT         72
FT                   /note="R -> RTG (in strain: 124; confers resistance to
FT                   erythromycin and azithromycin)"
SQ   SEQUENCE   207 AA;  22125 MW;  8376A6D812D4CE4B CRC64;
     MANVKLFDQT GKEVSSVELN DAIFGIEPNE SVVFDVVISQ RASLRQGTHA VKNRSAVSGG
     GRKPWRQKGT GRARQGSIRS PQWRGGGVVF GPTPRSYGYK LPQKVRRLAL KSVYSAKVAE
     DKFVAVEGLS FAAPKTAEFA KVLSALSIDT KVLVLVEEGN EFAALSARNL PNVTVATAAT
     ASVLDIVNAD KLLVTKEAIS TIEEVLA