RL4_THET8
ID RL4_THET8 Reviewed; 210 AA.
AC Q5SHN9;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=50S ribosomal protein L4;
DE AltName: Full=L1e;
GN Name=rplD; OrderedLocusNames=TTHA1691;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8566766; DOI=10.1016/0378-1119(95)00698-2;
RA Pfeiffer T., Jorcke D., Feltens R., Hartmann R.K.;
RT "Direct linkage of str-, S10- and spc-related gene clusters in Thermus
RT thermophilus HB8, and sequences of ribosomal proteins L4 and S10.";
RL Gene 167:141-145(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 6-17, AND BLOCKAGE OF N-TERMINUS.
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=11154066; DOI=10.1515/bc.2000.133;
RA Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H.,
RA Choli-Papadopoulou T.;
RT "Identification of the 50S ribosomal proteins from the eubacterium Thermus
RT thermophilus.";
RL Biol. Chem. 381:1079-1087(2000).
RN [4]
RP MUTAGENESIS OF GLY-55 AND GLU-56.
RX PubMed=12946348; DOI=10.1016/s0022-2836(03)00900-8;
RA Leontiadou F., Xaplanteri M.A., Papadopoulos G., Gerassimou C.,
RA Kalpaxis D.L., Choli-Papadopoulou T.;
RT "On the structural and functional importance of the highly conserved Glu56
RT of Thermus thermophilus L4 ribosomal protein.";
RL J. Mol. Biol. 332:73-84(2003).
RN [5]
RP MASS SPECTROMETRY.
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=16287167; DOI=10.1002/pmic.200402111;
RA Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.;
RT "Extending ribosomal protein identifications to unsequenced bacterial
RT strains using matrix-assisted laser desorption/ionization mass
RT spectrometry.";
RL Proteomics 5:4818-4831(2005).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
RX PubMed=11511350; DOI=10.1016/s0092-8674(01)00435-4;
RA Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.;
RT "The path of messenger RNA through the ribosome.";
RL Cell 106:233-241(2001).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
RX PubMed=11283358; DOI=10.1126/science.1060089;
RA Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N.,
RA Cate J.H.D., Noller H.F.;
RT "Crystal structure of the ribosome at 5.5 A resolution.";
RL Science 292:883-896(2001).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (3.45 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX WITH RF2,
RP AND SUBUNIT.
RX PubMed=18988853; DOI=10.1126/science.1164840;
RA Weixlbaumer A., Jin H., Neubauer C., Voorhees R.M., Petry S., Kelley A.C.,
RA Ramakrishnan V.;
RT "Insights into translational termination from the structure of RF2 bound to
RT the ribosome.";
RL Science 322:953-956(2008).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX WITH RF2,
RP AND SUBUNIT.
RX PubMed=20421507; DOI=10.1073/pnas.1003995107;
RA Jin H., Kelley A.C., Loakes D., Ramakrishnan V.;
RT "Structure of the 70S ribosome bound to release factor 2 and a substrate
RT analog provides insights into catalysis of peptide release.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:8593-8598(2010).
CC -!- FUNCTION: One of the primary rRNA binding proteins, this protein
CC initially binds near the 5'-end of the 23S rRNA. It is important during
CC the early stages of 50S assembly. It makes multiple contacts with
CC different domains of the 23S rRNA in the assembled 50S subunit and
CC ribosome (By similarity). {ECO:0000250}.
CC -!- FUNCTION: Forms part of the polypeptide exit tunnel. {ECO:0000250}.
CC -!- FUNCTION: This protein can be incorporated into E.coli ribosomes in
CC vivo, which resulted in decreased peptidyltransferase (Ptase) activity
CC of the hybrid ribosomes. The hybrid 50S subunits associate less well
CC with 30S subunits to form the ribosome.
CC -!- SUBUNIT: Part of the 50S ribosomal subunit.
CC -!- PTM: The N-terminus is blocked.
CC -!- MASS SPECTROMETRY: Mass=22724; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:16287167};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL4 family.
CC {ECO:0000305}.
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DR EMBL; U36480; AAA97862.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD71514.1; -; Genomic_DNA.
DR RefSeq; WP_008633422.1; NC_006461.1.
DR RefSeq; YP_144957.2; NC_006461.1.
DR PDB; 1VVJ; X-ray; 3.44 A; RF/YF=1-210.
DR PDB; 1VY4; X-ray; 2.60 A; BF/DF=1-210.
DR PDB; 1VY5; X-ray; 2.55 A; BF/DF=1-210.
DR PDB; 1VY6; X-ray; 2.90 A; BF/DF=1-210.
DR PDB; 1VY7; X-ray; 2.80 A; BF/DF=1-210.
DR PDB; 4L47; X-ray; 3.22 A; RF/YF=1-210.
DR PDB; 4L71; X-ray; 3.90 A; RF/YF=1-210.
DR PDB; 4LEL; X-ray; 3.90 A; RF/YF=1-210.
DR PDB; 4LFZ; X-ray; 3.92 A; RF/YF=1-210.
DR PDB; 4LNT; X-ray; 2.94 A; RF/YF=1-210.
DR PDB; 4LSK; X-ray; 3.48 A; RF/YF=1-210.
DR PDB; 4LT8; X-ray; 3.14 A; RF/YF=1-210.
DR PDB; 4P6F; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 4P70; X-ray; 3.68 A; RF/YF=1-210.
DR PDB; 4TUA; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 4TUB; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 4TUC; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 4TUD; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 4TUE; X-ray; 3.50 A; RF/YF=1-210.
DR PDB; 4V42; X-ray; 5.50 A; BF=1-210.
DR PDB; 4V4P; X-ray; 5.50 A; BF=1-210.
DR PDB; 4V4X; X-ray; 5.00 A; BF=1-210.
DR PDB; 4V4Y; X-ray; 5.50 A; BF=1-210.
DR PDB; 4V4Z; X-ray; 4.51 A; BF=1-210.
DR PDB; 4V51; X-ray; 2.80 A; BF/DF=1-210.
DR PDB; 4V5A; X-ray; 3.50 A; BF/DF=1-210.
DR PDB; 4V5C; X-ray; 3.30 A; BF/DF=1-210.
DR PDB; 4V5D; X-ray; 3.50 A; BF/DF=1-210.
DR PDB; 4V5E; X-ray; 3.45 A; BF/DF=1-210.
DR PDB; 4V5F; X-ray; 3.60 A; BF/DF=1-210.
DR PDB; 4V5G; X-ray; 3.60 A; BF/DF=1-210.
DR PDB; 4V5J; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V5K; X-ray; 3.20 A; BF/DF=1-210.
DR PDB; 4V5L; X-ray; 3.10 A; BF=1-210.
DR PDB; 4V5M; EM; 7.80 A; BF=1-210.
DR PDB; 4V5N; EM; 7.60 A; BF=1-210.
DR PDB; 4V5P; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V5Q; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V5R; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V5S; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V68; EM; 6.40 A; BF=1-208.
DR PDB; 4V6A; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V6F; X-ray; 3.10 A; AF/DF=1-210.
DR PDB; 4V6G; X-ray; 3.50 A; BF/DF=1-210.
DR PDB; 4V7J; X-ray; 3.30 A; AF/BF=1-210.
DR PDB; 4V7K; X-ray; 3.60 A; AF/BF=1-210.
DR PDB; 4V7L; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V7M; X-ray; 3.45 A; BF/DF=1-210.
DR PDB; 4V7W; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V7X; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V7Y; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V7Z; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V87; X-ray; 3.10 A; AF/DF=1-208.
DR PDB; 4V8A; X-ray; 3.20 A; AF/BF=6-210.
DR PDB; 4V8B; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V8C; X-ray; 3.30 A; AF/BF=1-210.
DR PDB; 4V8D; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V8E; X-ray; 3.30 A; AF/CF=1-210.
DR PDB; 4V8F; X-ray; 3.30 A; AF/DF=1-210.
DR PDB; 4V8G; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V8H; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V8I; X-ray; 2.70 A; BF/DF=1-210.
DR PDB; 4V8J; X-ray; 3.90 A; BF/DF=1-210.
DR PDB; 4V8N; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V8O; X-ray; 3.80 A; BF=1-210.
DR PDB; 4V8Q; X-ray; 3.10 A; AF=1-210.
DR PDB; 4V8U; X-ray; 3.70 A; BF/DF=1-210.
DR PDB; 4V8X; X-ray; 3.35 A; BF/DF=1-210.
DR PDB; 4V90; X-ray; 2.95 A; BF=1-210.
DR PDB; 4V95; X-ray; 3.20 A; BF/DF=1-210.
DR PDB; 4V97; X-ray; 3.52 A; BF/DF=1-210.
DR PDB; 4V9A; X-ray; 3.30 A; BF/DF=1-210.
DR PDB; 4V9B; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4V9H; X-ray; 2.86 A; BF=1-210.
DR PDB; 4V9I; X-ray; 3.30 A; BF/DF=1-207.
DR PDB; 4V9R; X-ray; 3.00 A; BF/DF=1-210.
DR PDB; 4V9S; X-ray; 3.10 A; BF/DF=1-210.
DR PDB; 4W2E; X-ray; 2.90 A; F=6-210.
DR PDB; 4W2F; X-ray; 2.40 A; BF/DF=1-210.
DR PDB; 4W2G; X-ray; 2.55 A; BF/DF=1-210.
DR PDB; 4W2H; X-ray; 2.70 A; BF/DF=1-210.
DR PDB; 4W2I; X-ray; 2.70 A; BF/DF=1-210.
DR PDB; 4W4G; X-ray; 3.30 A; RF/YF=1-210.
DR PDB; 4WPO; X-ray; 2.80 A; AF/CF=1-210.
DR PDB; 4WQ1; X-ray; 3.10 A; 31=6-207, 39=1-210.
DR PDB; 4WQF; X-ray; 2.80 A; AF/CF=1-210.
DR PDB; 4WQR; X-ray; 3.15 A; 31/39=1-210.
DR PDB; 4WQU; X-ray; 2.80 A; AF/CF=1-210.
DR PDB; 4WQY; X-ray; 2.80 A; AF/CF=1-210.
DR PDB; 4WR6; X-ray; 3.05 A; 31/39=1-210.
DR PDB; 4WRA; X-ray; 3.05 A; 31/39=1-210.
DR PDB; 4WRO; X-ray; 3.05 A; 31=1-210.
DR PDB; 4WSD; X-ray; 2.95 A; 31/39=1-210.
DR PDB; 4WSM; X-ray; 3.30 A; 31/39=1-210.
DR PDB; 4WT1; X-ray; 3.05 A; 31/39=1-210.
DR PDB; 4WT8; X-ray; 3.40 A; CD/DD=1-207.
DR PDB; 4WU1; X-ray; 3.20 A; 31/39=1-210.
DR PDB; 4WZD; X-ray; 3.10 A; 31/39=1-210.
DR PDB; 4WZO; X-ray; 3.30 A; 31/39=1-210.
DR PDB; 4Y4O; X-ray; 2.30 A; 1F/2F=1-210.
DR PDB; 4Y4P; X-ray; 2.50 A; 1F/2F=1-210.
DR PDB; 4YPB; X-ray; 3.40 A; RF/YF=1-210.
DR PDB; 4YZV; X-ray; 3.10 A; RF/YF=1-210.
DR PDB; 4Z3S; X-ray; 2.65 A; 1F/2F=1-210.
DR PDB; 4Z8C; X-ray; 2.90 A; 1F/2F=1-210.
DR PDB; 4ZER; X-ray; 3.10 A; 1F/2F=6-208.
DR PDB; 4ZSN; X-ray; 3.60 A; RF/YF=1-210.
DR PDB; 5A9Z; EM; 4.70 A; AF=3-210.
DR PDB; 5AA0; EM; 5.00 A; AF=3-210.
DR PDB; 5CZP; X-ray; 3.30 A; RF/YF=1-210.
DR PDB; 5D8B; X-ray; 3.63 A; C/YA=7-210.
DR PDB; 5DFE; X-ray; 3.10 A; RF/YF=1-210.
DR PDB; 5DOX; X-ray; 3.10 A; 1F/2F=1-210.
DR PDB; 5DOY; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 5E7K; X-ray; 3.20 A; 31/39=1-210.
DR PDB; 5E81; X-ray; 2.95 A; 31/39=1-210.
DR PDB; 5EL4; X-ray; 3.15 A; 31/39=1-210.
DR PDB; 5EL5; X-ray; 3.15 A; 31/39=1-210.
DR PDB; 5EL6; X-ray; 3.10 A; 31/39=1-210.
DR PDB; 5EL7; X-ray; 3.15 A; 31/39=1-210.
DR PDB; 5F8K; X-ray; 2.80 A; 1F/2F=6-208.
DR PDB; 5FDU; X-ray; 2.90 A; 1F/2F=6-208.
DR PDB; 5FDV; X-ray; 2.80 A; 1F/2F=6-208.
DR PDB; 5HAU; X-ray; 3.00 A; 1F/2F=1-210.
DR PDB; 5HCP; X-ray; 2.89 A; 1F/2F=1-210.
DR PDB; 5HCQ; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 5HCR; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 5HD1; X-ray; 2.70 A; 1F/2F=1-210.
DR PDB; 5IB7; X-ray; 2.99 A; 31/39=1-210.
DR PDB; 5IB8; X-ray; 3.13 A; 31/39=1-210.
DR PDB; 5IBB; X-ray; 2.96 A; 31/39=1-210.
DR PDB; 5IMQ; EM; 3.80 A; c=1-210.
DR PDB; 5IMR; EM; -; c=1-210.
DR PDB; 5J30; X-ray; 3.20 A; RF/YF=1-210.
DR PDB; 5J3C; X-ray; 3.04 A; RF/YF=1-210.
DR PDB; 5J4B; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 5J4C; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 5J8B; X-ray; 2.60 A; F=1-210.
DR PDB; 5NDJ; X-ray; 3.15 A; 31/39=1-210.
DR PDB; 5NDK; X-ray; 2.95 A; 31/39=1-210.
DR PDB; 5OT7; EM; 3.80 A; i=1-208.
DR PDB; 5UQ7; EM; 3.50 A; F=6-208.
DR PDB; 5UQ8; EM; 3.20 A; F=6-208.
DR PDB; 5V8I; X-ray; 3.25 A; 1F/2F=1-210.
DR PDB; 5VP2; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 5VPO; X-ray; 3.34 A; RF/YF=1-210.
DR PDB; 5VPP; X-ray; 3.90 A; RF/YF=1-210.
DR PDB; 5W4K; X-ray; 2.70 A; 1F/2F=1-210.
DR PDB; 5WIS; X-ray; 2.70 A; 1F/2F=1-210.
DR PDB; 5WIT; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 5ZLU; EM; 3.60 A; b=1-210.
DR PDB; 6BUW; X-ray; 3.50 A; RF/YF=1-210.
DR PDB; 6BZ6; X-ray; 3.18 A; RF/YF=1-210.
DR PDB; 6BZ7; X-ray; 3.68 A; RF/YF=1-210.
DR PDB; 6BZ8; X-ray; 3.74 A; RF/YF=1-210.
DR PDB; 6C5L; X-ray; 3.20 A; BF/DF=1-210.
DR PDB; 6CAE; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 6CFJ; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 6CFK; X-ray; 2.70 A; 1F/2F=1-210.
DR PDB; 6CFL; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 6CZR; X-ray; 3.14 A; 1F/2F=6-208.
DR PDB; 6FKR; X-ray; 3.20 A; 1F/2F=6-208.
DR PDB; 6GSJ; X-ray; 2.96 A; 31/39=1-210.
DR PDB; 6GSK; X-ray; 3.36 A; 31/39=1-210.
DR PDB; 6GSL; X-ray; 3.16 A; 31/39=1-210.
DR PDB; 6GZQ; EM; 3.28 A; E1=1-208.
DR PDB; 6GZX; EM; 4.57 A; E1/E2=1-208.
DR PDB; 6GZZ; EM; 4.13 A; E1/E2=1-208.
DR PDB; 6ND5; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 6ND6; X-ray; 2.85 A; 1F/2F=1-210.
DR PDB; 6NDK; X-ray; 3.64 A; RF/YF=1-210.
DR PDB; 6NSH; X-ray; 3.40 A; RF/YF=1-210.
DR PDB; 6NTA; X-ray; 3.10 A; RF/YF=1-210.
DR PDB; 6NUO; X-ray; 3.20 A; RF/YF=1-210.
DR PDB; 6NWY; X-ray; 3.50 A; RF/YF=1-210.
DR PDB; 6O3M; X-ray; 3.97 A; RF/YF=1-210.
DR PDB; 6O97; X-ray; 2.75 A; 1F/2F=1-210.
DR PDB; 6OF1; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 6OF6; X-ray; 3.20 A; RF/YF=1-210.
DR PDB; 6OJ2; X-ray; 3.20 A; RF/YF=1-210.
DR PDB; 6OPE; X-ray; 3.10 A; RF/YF=1-210.
DR PDB; 6ORD; X-ray; 3.10 A; RF/YF=1-210.
DR PDB; 6OSI; X-ray; 4.14 A; RF/YF=1-210.
DR PDB; 6OTR; X-ray; 3.12 A; RF/YF=1-210.
DR PDB; 6OXA; X-ray; 3.25 A; RF/YF=1-210.
DR PDB; 6OXI; X-ray; 3.50 A; RF/YF=1-210.
DR PDB; 6Q95; EM; 3.70 A; D=1-208.
DR PDB; 6QNQ; X-ray; 3.50 A; 31/39=1-210.
DR PDB; 6QNR; X-ray; 3.10 A; 31/39=1-210.
DR PDB; 6UCQ; X-ray; 3.50 A; 1F/2F=1-210.
DR PDB; 6UO1; X-ray; 2.95 A; 1F/2F=1-210.
DR PDB; 6XHV; X-ray; 2.40 A; 1F/2F=1-210.
DR PDB; 6XHW; X-ray; 2.50 A; 1F/2F=1-210.
DR PDB; 6XHX; X-ray; 2.55 A; 1F/2F=1-210.
DR PDB; 6XHY; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 6XQD; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 6XQE; X-ray; 3.00 A; 1F/2F=1-210.
DR PDB; 7JQL; X-ray; 3.00 A; 1F/2F=1-210.
DR PDB; 7JQM; X-ray; 3.05 A; 1F/2F=1-210.
DR PDB; 7LH5; X-ray; 3.27 A; BF/DF=1-210.
DR PDB; 7MD7; X-ray; 2.80 A; 1F/2F=1-210.
DR PDB; 7RQ8; X-ray; 2.50 A; 1F/2F=1-210.
DR PDB; 7RQ9; X-ray; 2.60 A; 1F/2F=1-210.
DR PDB; 7RQA; X-ray; 2.40 A; 1F/2F=1-210.
DR PDB; 7RQB; X-ray; 2.45 A; 1F/2F=1-210.
DR PDB; 7RQC; X-ray; 2.50 A; 1F/2F=1-210.
DR PDB; 7RQD; X-ray; 2.50 A; 1F/2F=1-210.
DR PDB; 7RQE; X-ray; 2.40 A; 1F/2F=1-210.
DR PDBsum; 1VVJ; -.
DR PDBsum; 1VY4; -.
DR PDBsum; 1VY5; -.
DR PDBsum; 1VY6; -.
DR PDBsum; 1VY7; -.
DR PDBsum; 4L47; -.
DR PDBsum; 4L71; -.
DR PDBsum; 4LEL; -.
DR PDBsum; 4LFZ; -.
DR PDBsum; 4LNT; -.
DR PDBsum; 4LSK; -.
DR PDBsum; 4LT8; -.
DR PDBsum; 4P6F; -.
DR PDBsum; 4P70; -.
DR PDBsum; 4TUA; -.
DR PDBsum; 4TUB; -.
DR PDBsum; 4TUC; -.
DR PDBsum; 4TUD; -.
DR PDBsum; 4TUE; -.
DR PDBsum; 4V42; -.
DR PDBsum; 4V4P; -.
DR PDBsum; 4V4X; -.
DR PDBsum; 4V4Y; -.
DR PDBsum; 4V4Z; -.
DR PDBsum; 4V51; -.
DR PDBsum; 4V5A; -.
DR PDBsum; 4V5C; -.
DR PDBsum; 4V5D; -.
DR PDBsum; 4V5E; -.
DR PDBsum; 4V5F; -.
DR PDBsum; 4V5G; -.
DR PDBsum; 4V5J; -.
DR PDBsum; 4V5K; -.
DR PDBsum; 4V5L; -.
DR PDBsum; 4V5M; -.
DR PDBsum; 4V5N; -.
DR PDBsum; 4V5P; -.
DR PDBsum; 4V5Q; -.
DR PDBsum; 4V5R; -.
DR PDBsum; 4V5S; -.
DR PDBsum; 4V68; -.
DR PDBsum; 4V6A; -.
DR PDBsum; 4V6F; -.
DR PDBsum; 4V6G; -.
DR PDBsum; 4V7J; -.
DR PDBsum; 4V7K; -.
DR PDBsum; 4V7L; -.
DR PDBsum; 4V7M; -.
DR PDBsum; 4V7W; -.
DR PDBsum; 4V7X; -.
DR PDBsum; 4V7Y; -.
DR PDBsum; 4V7Z; -.
DR PDBsum; 4V87; -.
DR PDBsum; 4V8A; -.
DR PDBsum; 4V8B; -.
DR PDBsum; 4V8C; -.
DR PDBsum; 4V8D; -.
DR PDBsum; 4V8E; -.
DR PDBsum; 4V8F; -.
DR PDBsum; 4V8G; -.
DR PDBsum; 4V8H; -.
DR PDBsum; 4V8I; -.
DR PDBsum; 4V8J; -.
DR PDBsum; 4V8N; -.
DR PDBsum; 4V8O; -.
DR PDBsum; 4V8Q; -.
DR PDBsum; 4V8U; -.
DR PDBsum; 4V8X; -.
DR PDBsum; 4V90; -.
DR PDBsum; 4V95; -.
DR PDBsum; 4V97; -.
DR PDBsum; 4V9A; -.
DR PDBsum; 4V9B; -.
DR PDBsum; 4V9H; -.
DR PDBsum; 4V9I; -.
DR PDBsum; 4V9R; -.
DR PDBsum; 4V9S; -.
DR PDBsum; 4W2E; -.
DR PDBsum; 4W2F; -.
DR PDBsum; 4W2G; -.
DR PDBsum; 4W2H; -.
DR PDBsum; 4W2I; -.
DR PDBsum; 4W4G; -.
DR PDBsum; 4WPO; -.
DR PDBsum; 4WQ1; -.
DR PDBsum; 4WQF; -.
DR PDBsum; 4WQR; -.
DR PDBsum; 4WQU; -.
DR PDBsum; 4WQY; -.
DR PDBsum; 4WR6; -.
DR PDBsum; 4WRA; -.
DR PDBsum; 4WRO; -.
DR PDBsum; 4WSD; -.
DR PDBsum; 4WSM; -.
DR PDBsum; 4WT1; -.
DR PDBsum; 4WT8; -.
DR PDBsum; 4WU1; -.
DR PDBsum; 4WZD; -.
DR PDBsum; 4WZO; -.
DR PDBsum; 4Y4O; -.
DR PDBsum; 4Y4P; -.
DR PDBsum; 4YPB; -.
DR PDBsum; 4YZV; -.
DR PDBsum; 4Z3S; -.
DR PDBsum; 4Z8C; -.
DR PDBsum; 4ZER; -.
DR PDBsum; 4ZSN; -.
DR PDBsum; 5A9Z; -.
DR PDBsum; 5AA0; -.
DR PDBsum; 5CZP; -.
DR PDBsum; 5D8B; -.
DR PDBsum; 5DFE; -.
DR PDBsum; 5DOX; -.
DR PDBsum; 5DOY; -.
DR PDBsum; 5E7K; -.
DR PDBsum; 5E81; -.
DR PDBsum; 5EL4; -.
DR PDBsum; 5EL5; -.
DR PDBsum; 5EL6; -.
DR PDBsum; 5EL7; -.
DR PDBsum; 5F8K; -.
DR PDBsum; 5FDU; -.
DR PDBsum; 5FDV; -.
DR PDBsum; 5HAU; -.
DR PDBsum; 5HCP; -.
DR PDBsum; 5HCQ; -.
DR PDBsum; 5HCR; -.
DR PDBsum; 5HD1; -.
DR PDBsum; 5IB7; -.
DR PDBsum; 5IB8; -.
DR PDBsum; 5IBB; -.
DR PDBsum; 5IMQ; -.
DR PDBsum; 5IMR; -.
DR PDBsum; 5J30; -.
DR PDBsum; 5J3C; -.
DR PDBsum; 5J4B; -.
DR PDBsum; 5J4C; -.
DR PDBsum; 5J8B; -.
DR PDBsum; 5NDJ; -.
DR PDBsum; 5NDK; -.
DR PDBsum; 5OT7; -.
DR PDBsum; 5UQ7; -.
DR PDBsum; 5UQ8; -.
DR PDBsum; 5V8I; -.
DR PDBsum; 5VP2; -.
DR PDBsum; 5VPO; -.
DR PDBsum; 5VPP; -.
DR PDBsum; 5W4K; -.
DR PDBsum; 5WIS; -.
DR PDBsum; 5WIT; -.
DR PDBsum; 5ZLU; -.
DR PDBsum; 6BUW; -.
DR PDBsum; 6BZ6; -.
DR PDBsum; 6BZ7; -.
DR PDBsum; 6BZ8; -.
DR PDBsum; 6C5L; -.
DR PDBsum; 6CAE; -.
DR PDBsum; 6CFJ; -.
DR PDBsum; 6CFK; -.
DR PDBsum; 6CFL; -.
DR PDBsum; 6CZR; -.
DR PDBsum; 6FKR; -.
DR PDBsum; 6GSJ; -.
DR PDBsum; 6GSK; -.
DR PDBsum; 6GSL; -.
DR PDBsum; 6GZQ; -.
DR PDBsum; 6GZX; -.
DR PDBsum; 6GZZ; -.
DR PDBsum; 6ND5; -.
DR PDBsum; 6ND6; -.
DR PDBsum; 6NDK; -.
DR PDBsum; 6NSH; -.
DR PDBsum; 6NTA; -.
DR PDBsum; 6NUO; -.
DR PDBsum; 6NWY; -.
DR PDBsum; 6O3M; -.
DR PDBsum; 6O97; -.
DR PDBsum; 6OF1; -.
DR PDBsum; 6OF6; -.
DR PDBsum; 6OJ2; -.
DR PDBsum; 6OPE; -.
DR PDBsum; 6ORD; -.
DR PDBsum; 6OSI; -.
DR PDBsum; 6OTR; -.
DR PDBsum; 6OXA; -.
DR PDBsum; 6OXI; -.
DR PDBsum; 6Q95; -.
DR PDBsum; 6QNQ; -.
DR PDBsum; 6QNR; -.
DR PDBsum; 6UCQ; -.
DR PDBsum; 6UO1; -.
DR PDBsum; 6XHV; -.
DR PDBsum; 6XHW; -.
DR PDBsum; 6XHX; -.
DR PDBsum; 6XHY; -.
DR PDBsum; 6XQD; -.
DR PDBsum; 6XQE; -.
DR PDBsum; 7JQL; -.
DR PDBsum; 7JQM; -.
DR PDBsum; 7LH5; -.
DR PDBsum; 7MD7; -.
DR PDBsum; 7RQ8; -.
DR PDBsum; 7RQ9; -.
DR PDBsum; 7RQA; -.
DR PDBsum; 7RQB; -.
DR PDBsum; 7RQC; -.
DR PDBsum; 7RQD; -.
DR PDBsum; 7RQE; -.
DR AlphaFoldDB; Q5SHN9; -.
DR SMR; Q5SHN9; -.
DR IntAct; Q5SHN9; 8.
DR STRING; 300852.55773073; -.
DR EnsemblBacteria; BAD71514; BAD71514; BAD71514.
DR GeneID; 3167923; -.
DR KEGG; ttj:TTHA1691; -.
DR PATRIC; fig|300852.9.peg.1661; -.
DR eggNOG; COG0088; Bacteria.
DR HOGENOM; CLU_041575_5_1_0; -.
DR OMA; PQVHILE; -.
DR EvolutionaryTrace; Q5SHN9; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1370.10; -; 1.
DR HAMAP; MF_01328_B; Ribosomal_L4_B; 1.
DR InterPro; IPR002136; Ribosomal_L4/L1e.
DR InterPro; IPR023574; Ribosomal_L4_dom_sf.
DR InterPro; IPR013005; Ribosomal_uL4/L1e.
DR PANTHER; PTHR10746; PTHR10746; 1.
DR Pfam; PF00573; Ribosomal_L4; 1.
DR SUPFAM; SSF52166; SSF52166; 1.
DR TIGRFAMs; TIGR03953; rplD_bact; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
FT CHAIN 1..210
FT /note="50S ribosomal protein L4"
FT /id="PRO_0000129300"
FT MUTAGEN 55
FT /note="G->A: Increases Ptase activity of hybrid ribosomes
FT to a value greater than that of pure E.coli ribosomes.
FT Increases binding of a tRNA analog to the P site."
FT /evidence="ECO:0000269|PubMed:12946348"
FT MUTAGEN 55
FT /note="G->S: No effect on Ptase activity of hybrid
FT ribosomes. No change in binding of a tRNA analog to the A
FT or P site."
FT /evidence="ECO:0000269|PubMed:12946348"
FT MUTAGEN 56
FT /note="E->A: Further decreases Ptase activity in E.coli
FT hybrid ribosomes. Significantly decreases binding of a tRNA
FT analog to the A or P site."
FT /evidence="ECO:0000269|PubMed:12946348"
FT MUTAGEN 56
FT /note="E->Q: Restores Ptase activity of hybrid ribosomes to
FT that of pure E.coli ribosomes. Significantly increases
FT binding of a tRNA analog to the P site."
FT /evidence="ECO:0000269|PubMed:12946348"
FT CONFLICT 19..20
FT /note="EL -> DV (in Ref. 1; AAA97862)"
FT /evidence="ECO:0000305"
FT STRAND 10..12
FT /evidence="ECO:0007829|PDB:4WT8"
FT TURN 22..25
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 30..43
FT /evidence="ECO:0007829|PDB:4WT8"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 103..120
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 136..145
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 161..166
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 178..180
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 183..188
FT /evidence="ECO:0007829|PDB:4WT8"
FT STRAND 189..194
FT /evidence="ECO:0007829|PDB:4WT8"
FT HELIX 196..204
FT /evidence="ECO:0007829|PDB:4WT8"
SQ SEQUENCE 210 AA; 23235 MW; BFB99AC179C110B4 CRC64;
MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS
GRKIWPQKHT GRARHGDIGA PIFVGGGVVF GPKPRDYSYT LPKKVRKKGL AMAVADRARE
GKLLLVEAFA GVNGKTKEFL AWAKEAGLDG SESVLLVTGN ELVRRAARNL PWVVTLAPEG
LNVYDIVRTE RLVMDLDAWE VFQNRIGGEA
