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RL6B_YEAST
ID   RL6B_YEAST              Reviewed;         176 AA.
AC   P05739; D6VZ82;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   03-AUG-2022, entry version 189.
DE   RecName: Full=60S ribosomal protein L6-B {ECO:0000303|PubMed:9559554};
DE   AltName: Full=L17;
DE   AltName: Full=Large ribosomal subunit protein eL6-B {ECO:0000303|PubMed:24524803};
DE   AltName: Full=RP18;
DE   AltName: Full=YL16;
GN   Name=RPL6B {ECO:0000303|PubMed:9559554}; Synonyms=RPL16B, RPL17B, YL16B;
GN   OrderedLocusNames=YLR448W; ORFNames=L9324.4;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1390890; DOI=10.1016/0167-4781(92)90011-n;
RA   Hashimoto T., Suzuki K., Mizuta K., Otaka E.;
RT   "Yeast ribosomal proteins: XIV. Complete nucleotide sequences of the two
RT   genes encoding Saccharomyces cerevisiae YL16.";
RL   Biochim. Biophys. Acta 1132:195-198(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   PARTIAL PROTEIN SEQUENCE OF 2-25, AND CLEAVAGE OF INITIATOR METHIONINE.
RX   PubMed=18782943; DOI=10.1007/bf00341461;
RA   Otaka E., Higo K., Itoh T.;
RT   "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence
RT   characterization of twenty-four proteins from cytoplasmic ribosomes.";
RL   Mol. Gen. Genet. 195:544-546(1984).
RN   [5]
RP   NOMENCLATURE, AND SUBUNIT.
RX   PubMed=9559554;
RX   DOI=10.1002/(sici)1097-0061(19980330)14:5<471::aid-yea241>3.0.co;2-u;
RA   Planta R.J., Mager W.H.;
RT   "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.";
RL   Yeast 14:471-477(1998).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=22096102; DOI=10.1126/science.1212642;
RA   Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G.,
RA   Yusupov M.;
RT   "The structure of the eukaryotic ribosome at 3.0 A resolution.";
RL   Science 334:1524-1529(2011).
RN   [9]
RP   UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-128, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22106047; DOI=10.1002/pmic.201100166;
RA   Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT   "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL   Proteomics 12:236-240(2012).
RN   [10]
RP   NOMENCLATURE.
RX   PubMed=24524803; DOI=10.1016/j.sbi.2014.01.002;
RA   Ban N., Beckmann R., Cate J.H.D., Dinman J.D., Dragon F., Ellis S.R.,
RA   Lafontaine D.L.J., Lindahl L., Liljas A., Lipton J.M., McAlear M.A.,
RA   Moore P.B., Noller H.F., Ortega J., Panse V.G., Ramakrishnan V.,
RA   Spahn C.M.T., Steitz T.A., Tchorzewski M., Tollervey D., Warren A.J.,
RA   Williamson J.R., Wilson D., Yonath A., Yusupov M.;
RT   "A new system for naming ribosomal proteins.";
RL   Curr. Opin. Struct. Biol. 24:165-169(2014).
CC   -!- FUNCTION: Component of the ribosome, a large ribonucleoprotein complex
CC       responsible for the synthesis of proteins in the cell. The small
CC       ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the
CC       encoded message by selecting cognate aminoacyl-transfer RNA (tRNA)
CC       molecules. The large subunit (LSU) contains the ribosomal catalytic
CC       site termed the peptidyl transferase center (PTC), which catalyzes the
CC       formation of peptide bonds, thereby polymerizing the amino acids
CC       delivered by tRNAs into a polypeptide chain. The nascent polypeptides
CC       leave the ribosome through a tunnel in the LSU and interact with
CC       protein factors that function in enzymatic processing, targeting, and
CC       the membrane insertion of nascent chains at the exit of the ribosomal
CC       tunnel. {ECO:0000305|PubMed:22096102}.
CC   -!- SUBUNIT: Component of the large ribosomal subunit (LSU). Mature yeast
CC       ribosomes consist of a small (40S) and a large (60S) subunit. The 40S
CC       small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33
CC       different proteins (encoded by 57 genes). The large 60S subunit
CC       contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different
CC       proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).
CC       {ECO:0000269|PubMed:22096102, ECO:0000305|PubMed:9559554}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:22096102}.
CC   -!- MISCELLANEOUS: Present with 38400 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- MISCELLANEOUS: There are 2 genes for eL6 in yeast. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL6 family.
CC       {ECO:0000305}.
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DR   EMBL; D10226; BAA01078.1; -; Genomic_DNA.
DR   EMBL; U22382; AAB67529.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09748.1; -; Genomic_DNA.
DR   PIR; S55970; S55970.
DR   RefSeq; NP_013553.1; NM_001182336.1.
DR   PDB; 6SNT; EM; 2.80 A; l=2-176.
DR   PDB; 6T4Q; EM; 2.60 A; LE=2-176.
DR   PDB; 6T7I; EM; 3.20 A; LE=1-176.
DR   PDB; 6T7T; EM; 3.10 A; LE=1-176.
DR   PDB; 6TB3; EM; 2.80 A; BM=2-176.
DR   PDB; 6TNU; EM; 3.10 A; BM=2-176.
DR   PDB; 7B7D; EM; 3.30 A; LH=2-176.
DR   PDB; 7NRC; EM; 3.90 A; LH=2-176.
DR   PDB; 7NRD; EM; 4.36 A; LH=2-176.
DR   PDBsum; 6SNT; -.
DR   PDBsum; 6T4Q; -.
DR   PDBsum; 6T7I; -.
DR   PDBsum; 6T7T; -.
DR   PDBsum; 6TB3; -.
DR   PDBsum; 6TNU; -.
DR   PDBsum; 7B7D; -.
DR   PDBsum; 7NRC; -.
DR   PDBsum; 7NRD; -.
DR   AlphaFoldDB; P05739; -.
DR   SMR; P05739; -.
DR   BioGRID; 31706; 385.
DR   DIP; DIP-4591N; -.
DR   IntAct; P05739; 29.
DR   MINT; P05739; -.
DR   STRING; 4932.YLR448W; -.
DR   iPTMnet; P05739; -.
DR   MaxQB; P05739; -.
DR   PaxDb; P05739; -.
DR   PRIDE; P05739; -.
DR   EnsemblFungi; YLR448W_mRNA; YLR448W; YLR448W.
DR   GeneID; 851169; -.
DR   KEGG; sce:YLR448W; -.
DR   SGD; S000004440; RPL6B.
DR   VEuPathDB; FungiDB:YLR448W; -.
DR   eggNOG; KOG1694; Eukaryota.
DR   GeneTree; ENSGT00390000003682; -.
DR   HOGENOM; CLU_066767_2_1_1; -.
DR   InParanoid; P05739; -.
DR   OMA; KHPLNSY; -.
DR   BioCyc; YEAST:G3O-32503-MON; -.
DR   Reactome; R-SCE-156827; L13a-mediated translational silencing of Ceruloplasmin expression.
DR   Reactome; R-SCE-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR   Reactome; R-SCE-72689; Formation of a pool of free 40S subunits.
DR   Reactome; R-SCE-72706; GTP hydrolysis and joining of the 60S ribosomal subunit.
DR   Reactome; R-SCE-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
DR   Reactome; R-SCE-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   PRO; PR:P05739; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; P05739; protein.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0022625; C:cytosolic large ribosomal subunit; IDA:SGD.
DR   GO; GO:0003723; F:RNA binding; IDA:SGD.
DR   GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD.
DR   GO; GO:0002181; P:cytoplasmic translation; IDA:SGD.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IMP:SGD.
DR   CDD; cd13156; KOW_RPL6; 1.
DR   Gene3D; 2.30.30.30; -; 1.
DR   InterPro; IPR000915; 60S_ribosomal_L6E.
DR   InterPro; IPR041997; KOW_RPL6.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR10715; PTHR10715; 1.
DR   Pfam; PF01159; Ribosomal_L6e; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   PROSITE; PS01170; RIBOSOMAL_L6E; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Direct protein sequencing; Isopeptide bond;
KW   Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW   Ubl conjugation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:18782943"
FT   CHAIN           2..176
FT                   /note="60S ribosomal protein L6-B"
FT                   /id="PRO_0000171018"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         12
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q02326"
FT   CROSSLNK        128
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0007744|PubMed:22106047"
FT   CONFLICT        68
FT                   /note="P -> L (in Ref. 1; BAA01078)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   176 AA;  19986 MW;  451848C3B1953BC4 CRC64;
     MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED
     NTLLVTGPFK VNGVPLRRVN ARYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAN
     LFPEQQTKEI KTERVEDQKV VDKALLAEIK KTPLLKQYLS ASFSLKNGDK PHLLKF
 
 
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