RL7_GEOSE
ID RL7_GEOSE Reviewed; 122 AA.
AC P05392;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=50S ribosomal protein L7/L12 {ECO:0000255|HAMAP-Rule:MF_00368};
DE AltName: Full=BL13;
DE AltName: Full=Ribosomal protein 'A';
GN Name=rplL {ECO:0000255|HAMAP-Rule:MF_00368};
OS Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1422;
RN [1]
RP PROTEIN SEQUENCE.
RX PubMed=3609321; DOI=10.1016/0014-5793(87)80872-4;
RA Garland W.G., Louie K.A., Matheson A.T., Liljas A.;
RT "The complete amino acid sequence of the ribosomal 'A' protein (L12) from
RT Bacillus stearothermophilus.";
RL FEBS Lett. 220:43-46(1987).
RN [2]
RP PROTEIN SEQUENCE OF 1-37.
RX PubMed=476516; DOI=10.1139/o79-090;
RA Visentin L.P., Yaguchi M., Matheson A.T.;
RT "Structural homologies in alanine-rich acidic ribosomal proteins from
RT procaryotes and eucaryotes.";
RL Can. J. Biochem. 57:719-726(1979).
RN [3]
RP SUBUNIT, STOICHIOMETRY, AND MASS SPECTROMETRY.
RX PubMed=20467040; DOI=10.1074/mcp.m000072-mcp201;
RA Gordiyenko Y., Videler H., Zhou M., McKay A.R., Fucini P., Biegel E.,
RA Muller V., Robinson C.V.;
RT "Mass spectrometry defines the stoichiometry of ribosomal stalk complexes
RT across the phylogenetic tree.";
RL Mol. Cell. Proteomics 9:1774-1783(2010).
CC -!- FUNCTION: Forms part of the ribosomal stalk which helps the ribosome
CC interact with GTP-bound translation factors. Is thus essential for
CC accurate translation (Probable). {ECO:0000305}.
CC -!- SUBUNIT: Homodimer. Part of the 50S ribosomal subunit; present in 4
CC copies per ribosome. Forms part of the ribosomal stalk which helps the
CC ribosome interact with GTP-bound translation factors. Forms a
CC pentameric L10(L12)2(L12)2 complex, where L10 forms an elongated spine
CC to which 2 L12 dimers bind in a sequential fashion.
CC {ECO:0000269|PubMed:20467040}.
CC -!- MASS SPECTROMETRY: Mass=69481.25; Mass_error=26.4; Method=Electrospray;
CC Note=Isolated L10(L12)4, grown at 55 degrees Celsius.;
CC Evidence={ECO:0000269|PubMed:20467040};
CC -!- MASS SPECTROMETRY: Mass=12835.60; Mass_error=1; Method=Electrospray;
CC Note=Grown at 55 degrees Celsius.;
CC Evidence={ECO:0000269|PubMed:20467040};
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL12 family.
CC {ECO:0000255|HAMAP-Rule:MF_00368}.
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DR PIR; S00076; R5BS12.
DR AlphaFoldDB; P05392; -.
DR SMR; P05392; -.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00387; Ribosomal_L7_L12; 1.
DR Gene3D; 1.20.5.710; -; 1.
DR Gene3D; 3.30.1390.10; -; 1.
DR HAMAP; MF_00368; Ribosomal_L7_L12; 1.
DR InterPro; IPR000206; Ribosomal_L7/12.
DR InterPro; IPR014719; Ribosomal_L7/12_C/ClpS-like.
DR InterPro; IPR013823; Ribosomal_L7/L12_C.
DR InterPro; IPR008932; Ribosomal_L7/L12_oligo.
DR InterPro; IPR036235; Ribosomal_L7/L12_oligo_N_sf.
DR PANTHER; PTHR45987; PTHR45987; 1.
DR Pfam; PF00542; Ribosomal_L12; 1.
DR Pfam; PF16320; Ribosomal_L12_N; 1.
DR SUPFAM; SSF48300; SSF48300; 1.
DR SUPFAM; SSF54736; SSF54736; 1.
DR TIGRFAMs; TIGR00855; L12; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..122
FT /note="50S ribosomal protein L7/L12"
FT /id="PRO_0000157504"
FT CONFLICT 8
FT /note="E -> Q (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 122 AA; 12780 MW; 5E69E87ADA2CF464 CRC64;
MTKEQIIEAV KNMTVLELNE LVKAIEEEFG VTAAAPVVVA GGAAAGAEAA AEKTEFDVIL
ADAGAQKIKV IKVVREITGL GLKEAKDLVD NTPKPIKEGI AKEEAEEIKA ALEEAGAKVE
IK
