RL81_ARATH
ID RL81_ARATH Reviewed; 258 AA.
AC P46286; Q9SL44;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 25-MAY-2022, entry version 157.
DE RecName: Full=60S ribosomal protein L8-1;
DE AltName: Full=60S ribosomal protein L2;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2296;
GN Name=RPL8A; Synonyms=EMB2296, RPL2; OrderedLocusNames=At2g18020;
GN ORFNames=T27K22.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24;
RA Schuster W.;
RL Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
RX PubMed=11598216; DOI=10.1104/pp.010265;
RA Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R.,
RA Delseny M., Bailey-Serres J.;
RT "The organization of cytoplasmic ribosomal protein genes in the Arabidopsis
RT genome.";
RL Plant Physiol. 127:398-415(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL2 family.
CC {ECO:0000305}.
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DR EMBL; X86765; CAA60445.1; -; mRNA.
DR EMBL; AC006201; AAD20124.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06716.1; -; Genomic_DNA.
DR EMBL; AY128314; AAM91517.1; -; mRNA.
DR EMBL; BT001177; AAN65064.1; -; mRNA.
DR PIR; C84559; C84559.
DR RefSeq; NP_179393.1; NM_127358.6.
DR AlphaFoldDB; P46286; -.
DR SMR; P46286; -.
DR BioGRID; 1671; 180.
DR IntAct; P46286; 12.
DR STRING; 3702.AT2G18020.1; -.
DR iPTMnet; P46286; -.
DR PaxDb; P46286; -.
DR PRIDE; P46286; -.
DR ProteomicsDB; 228120; -.
DR EnsemblPlants; AT2G18020.1; AT2G18020.1; AT2G18020.
DR GeneID; 816314; -.
DR Gramene; AT2G18020.1; AT2G18020.1; AT2G18020.
DR KEGG; ath:AT2G18020; -.
DR Araport; AT2G18020; -.
DR TAIR; locus:2060917; AT2G18020.
DR eggNOG; KOG2309; Eukaryota.
DR HOGENOM; CLU_036235_0_3_1; -.
DR InParanoid; P46286; -.
DR OMA; HPYKFKM; -.
DR OrthoDB; 1156335at2759; -.
DR PhylomeDB; P46286; -.
DR PRO; PR:P46286; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P46286; baseline and differential.
DR Genevisible; P46286; AT.
DR GO; GO:0022625; C:cytosolic large ribosomal subunit; HDA:TAIR.
DR GO; GO:0022626; C:cytosolic ribosome; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0042788; C:polysomal ribosome; IDA:CAFA.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:CAFA.
DR GO; GO:0002181; P:cytoplasmic translation; IBA:GO_Central.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 4.10.950.10; -; 1.
DR HAMAP; MF_01320_A; Ribosomal_L2_A; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR022666; Rbsml_prot_L2_RNA-bd_dom.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR002171; Ribosomal_L2.
DR InterPro; IPR023672; Ribosomal_L2_arc.
DR InterPro; IPR022669; Ribosomal_L2_C.
DR InterPro; IPR022671; Ribosomal_L2_CS.
DR InterPro; IPR014726; Ribosomal_L2_dom3.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR13691; PTHR13691; 1.
DR Pfam; PF00181; Ribosomal_L2; 1.
DR Pfam; PF03947; Ribosomal_L2_C; 1.
DR PIRSF; PIRSF002158; Ribosomal_L2; 1.
DR SMART; SM01383; Ribosomal_L2; 1.
DR SMART; SM01382; Ribosomal_L2_C; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS00467; RIBOSOMAL_L2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Ribonucleoprotein; Ribosomal protein.
FT CHAIN 1..258
FT /note="60S ribosomal protein L8-1"
FT /id="PRO_0000129733"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 81..87
FT /note="GMYTGQF -> VCTPVSS (in Ref. 1; CAA60445)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="H -> E (in Ref. 1; CAA60445)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="C -> S (in Ref. 1; CAA60445)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="A -> S (in Ref. 1; CAA60445)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 27859 MW; C88C5942DF9AC4B2 CRC64;
MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDFGERNGY LKGVVTEIIH DPGRGAPLAR
VTFRHPFRFK KQKELFVAAE GMYTGQFLYC GKKATLVVGN VLPLRSIPEG AVVCNVEHHV
GDRGVLARAS GDYAIVIAHN PDSDTTRIKL PSGSKKIVPS GCRAMIGQVA GGGRTEKPML
KAGNAYHKYR VKRNSWPKVR GVAMNPVEHP HGGGNHQHIG HASTVRRDAP PGQKVGLIAA
RRTGRLRGQA AASAAKAD
