ID   RLF18_ARATH             Reviewed;         103 AA.
AC   O49320;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Protein RALF-like 18;
DE   Flags: Precursor;
GN   Name=RALFL18; OrderedLocusNames=At2g33130; ORFNames=F25I18.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 1-80.
RC   STRAIN=cv. Columbia;
RA   Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA   Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- PTM: Proteolytically cleaved, probably by S1P, a subtilisin-like serine
CC       protease (subtilase). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AC002334; AAC04909.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08790.1; -; Genomic_DNA.
DR   EMBL; AY954812; AAX55138.1; -; Genomic_DNA.
DR   EMBL; DQ077903; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; G84741; G84741.
DR   RefSeq; NP_180872.1; NM_128873.2.
DR   AlphaFoldDB; O49320; -.
DR   SMR; O49320; -.
DR   PaxDb; O49320; -.
DR   PRIDE; O49320; -.
DR   EnsemblPlants; AT2G33130.1; AT2G33130.1; AT2G33130.
DR   GeneID; 817875; -.
DR   Gramene; AT2G33130.1; AT2G33130.1; AT2G33130.
DR   KEGG; ath:AT2G33130; -.
DR   Araport; AT2G33130; -.
DR   TAIR; locus:2046580; AT2G33130.
DR   HOGENOM; CLU_2295534_0_0_1; -.
DR   InParanoid; O49320; -.
DR   OMA; ATGCYRF; -.
DR   OrthoDB; 1512493at2759; -.
DR   PhylomeDB; O49320; -.
DR   PRO; PR:O49320; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O49320; baseline and differential.
DR   Genevisible; O49320; AT.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   PROPEP          33..58
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000420310"
FT   CHAIN           59..103
FT                   /note="Protein RALF-like 18"
FT                   /id="PRO_0000420311"
FT   SITE            55..56
FT                   /note="Required for proteolytic cleavage"
FT                   /evidence="ECO:0000250"
FT   DISULFID        92..98
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   103 AA;  11390 MW;  BB9F2261773CAD61 CRC64;
     MMNNMKLLII AVMIISAALL PALVVGSRPV KCDNCMDGGE KEEIMKMSSG VDVSHRILQA
     KRFIDYEALK KNLPAKPDGK PDKPDNKYRR GCSAATGCYR FTN