ID   RLF20_ARATH             Reviewed;          72 AA.
AC   A8MQL7;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Protein RALF-like 20;
DE   Flags: Precursor;
GN   Name=RALFL20; OrderedLocusNames=At2g34825; ORFNames=F19I3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AC004238; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002685; AEC09026.1; -; Genomic_DNA.
DR   RefSeq; NP_001318355.1; NM_001336529.1.
DR   AlphaFoldDB; A8MQL7; -.
DR   STRING; 3702.AT2G34825.1; -.
DR   PaxDb; A8MQL7; -.
DR   PRIDE; A8MQL7; -.
DR   ProteomicsDB; 228005; -.
DR   EnsemblPlants; AT2G34825.1; AT2G34825.1; AT2G34825.
DR   GeneID; 28718326; -.
DR   Gramene; AT2G34825.1; AT2G34825.1; AT2G34825.
DR   KEGG; ath:AT2G34825; -.
DR   Araport; AT2G34825; -.
DR   TAIR; locus:4010713678; AT2G34825.
DR   eggNOG; ENOG502T1XS; Eukaryota.
DR   HOGENOM; CLU_2815974_0_0_1; -.
DR   OMA; AMREDEP; -.
DR   OrthoDB; 1627876at2759; -.
DR   PhylomeDB; A8MQL7; -.
DR   PRO; PR:A8MQL7; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; A8MQL7; baseline.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..72
FT                   /note="Protein RALF-like 20"
FT                   /id="PRO_0000420314"
FT   DISULFID        43..51
FT                   /evidence="ECO:0000250"
FT   DISULFID        63..69
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   72 AA;  7717 MW;  387700ECBDD7BFD5 CRC64;
     MVLSKKTIMQ SFALMIILSI VMSTTEAKTI GNPAMREDEP KGCPPGSPAS CKMQPANPYK
     PGCEASQRCR GT