ID   RLF21_ARATH             Reviewed;         105 AA.
AC   A8MRF9;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Protein RALF-like 21;
DE   Flags: Precursor;
GN   Name=RALFL21; OrderedLocusNames=At3g04735; ORFNames=F7O18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds and rosettes.
CC       {ECO:0000269|PubMed:12611624}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AC011437; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002686; AEE74129.1; -; Genomic_DNA.
DR   RefSeq; NP_001078107.1; NM_001084638.2.
DR   AlphaFoldDB; A8MRF9; -.
DR   PaxDb; A8MRF9; -.
DR   PRIDE; A8MRF9; -.
DR   EnsemblPlants; AT3G04735.1; AT3G04735.1; AT3G04735.
DR   GeneID; 5007983; -.
DR   Gramene; AT3G04735.1; AT3G04735.1; AT3G04735.
DR   KEGG; ath:AT3G04735; -.
DR   Araport; AT3G04735; -.
DR   TAIR; locus:4010713730; AT3G04735.
DR   HOGENOM; CLU_189400_0_0_1; -.
DR   OMA; CVFISSM; -.
DR   OrthoDB; 1795293at2759; -.
DR   PhylomeDB; A8MRF9; -.
DR   PRO; PR:A8MRF9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; A8MRF9; baseline and differential.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..105
FT                   /note="Protein RALF-like 21"
FT                   /id="PRO_0000420315"
FT   DISULFID        44..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..73
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   105 AA;  11951 MW;  49064C029FCFB000 CRC64;
     MSNMKITNRF MLVATFIACV FISSMNMTVG KVIGYPGLKP DLPCDHHRYP SACAPSEQPV
     NPYRRGCSKI HRCRRDSPPA PISRKMLIRG QLIYNNAYNA YIQYP