RLF23_ARATH
ID RLF23_ARATH Reviewed; 138 AA.
AC Q9LUS7;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Rapid alkalinization factor 23;
DE Short=AtRALF23;
DE AltName: Full=Protein RALF-like 23;
DE Flags: Precursor;
GN Name=RALF23; Synonyms=RALFL23; OrderedLocusNames=At3g16570;
GN ORFNames=MGL6.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12611624;
RA Olsen A.N., Mundy J., Skriver K.;
RT "Peptomics, identification of novel cationic Arabidopsis peptides with
RT conserved sequence motifs.";
RL In Silico Biol. 2:441-451(2002).
RN [6]
RP FUNCTION, INDUCTION BY BRASSINOLIDE, DEVELOPMENTAL STAGE, CLEAVAGE BY
RP SBT6.1, AND MUTAGENESIS OF 85-ARG-ARG-86.
RX PubMed=19473327; DOI=10.1111/j.1365-313x.2009.03926.x;
RA Srivastava R., Liu J.-X., Guo H., Yin Y., Howell S.H.;
RT "Regulation and processing of a plant peptide hormone, AtRALF23, in
RT Arabidopsis.";
RL Plant J. 59:930-939(2009).
CC -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC growth, and development. Mediates a rapid alkalinization of
CC extracellular space by mediating a transient increase in the
CC cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC signaling events through a cell surface receptor and a concomitant
CC activation of some intracellular mitogen-activated protein kinases (By
CC similarity). Regulates negatively brassinolide (BL)-mediated signaling
CC pathway (e.g. BL-induced hypocotyl elongation and branching limitation)
CC (PubMed:19473327). {ECO:0000250, ECO:0000269|PubMed:19473327}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: In very young seedlings, confined to the tips of
CC the cotyledons. Later expressed in the cotyledon vasculature and
CC petiole. Present in the tip of emerging leaves. In mature leaves,
CC observed in the vasculature. {ECO:0000269|PubMed:19473327}.
CC -!- INDUCTION: Repressed by brassinolide (BL) treatment.
CC {ECO:0000269|PubMed:19473327}.
CC -!- PTM: Proteolytically cleaved, probably by SBT6.1 (S1P), a subtilisin-
CC like serine protease (subtilase). {ECO:0000269|PubMed:19473327}.
CC -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC family. {ECO:0000305}.
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DR EMBL; AB022217; BAB02748.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75839.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65229.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65230.1; -; Genomic_DNA.
DR EMBL; AY048301; AAK82563.1; -; mRNA.
DR EMBL; AY072630; AAL62021.1; -; mRNA.
DR EMBL; AY084588; AAM61153.1; -; mRNA.
DR RefSeq; NP_001319570.1; NM_001338245.1.
DR RefSeq; NP_001327214.1; NM_001338247.1.
DR RefSeq; NP_001327215.1; NM_001338246.1.
DR PDB; 6A5E; X-ray; 2.77 A; E/F=92-106.
DR PDBsum; 6A5E; -.
DR AlphaFoldDB; Q9LUS7; -.
DR SMR; Q9LUS7; -.
DR STRING; 3702.AT3G16570.1; -.
DR PaxDb; Q9LUS7; -.
DR PRIDE; Q9LUS7; -.
DR ProteomicsDB; 228157; -.
DR EnsemblPlants; AT3G16570.1; AT3G16570.1; AT3G16570.
DR EnsemblPlants; AT3G16570.2; AT3G16570.2; AT3G16570.
DR EnsemblPlants; AT3G16570.3; AT3G16570.3; AT3G16570.
DR GeneID; 820907; -.
DR Gramene; AT3G16570.1; AT3G16570.1; AT3G16570.
DR Gramene; AT3G16570.2; AT3G16570.2; AT3G16570.
DR Gramene; AT3G16570.3; AT3G16570.3; AT3G16570.
DR KEGG; ath:AT3G16570; -.
DR Araport; AT3G16570; -.
DR TAIR; locus:2089323; AT3G16570.
DR eggNOG; ENOG502S1TF; Eukaryota.
DR HOGENOM; CLU_127895_1_2_1; -.
DR InParanoid; Q9LUS7; -.
DR OMA; YSNCEAQ; -.
DR OrthoDB; 1601238at2759; -.
DR PhylomeDB; Q9LUS7; -.
DR PRO; PR:Q9LUS7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUS7; baseline and differential.
DR Genevisible; Q9LUS7; AT.
DR GO; GO:0048046; C:apoplast; ISS:TAIR.
DR GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR GO; GO:0045926; P:negative regulation of growth; IMP:TAIR.
DR GO; GO:0009741; P:response to brassinosteroid; IEP:TAIR.
DR InterPro; IPR008801; RALF.
DR Pfam; PF05498; RALF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Brassinosteroid signaling pathway; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT PROPEP 29..88
FT /note="Removed in mature form"
FT /evidence="ECO:0000269|PubMed:19473327"
FT /id="PRO_0000420318"
FT CHAIN 89..138
FT /note="Rapid alkalinization factor 23"
FT /id="PRO_0000420319"
FT SITE 85..86
FT /note="Required for proteolytic cleavage"
FT /evidence="ECO:0000305|PubMed:19473327"
FT DISULFID 106..116
FT /evidence="ECO:0000250"
FT DISULFID 129..135
FT /evidence="ECO:0000250"
FT MUTAGEN 85..86
FT /note="RR->GG: Loss of propeptide cleavage by kexin-like
FT convertase leading to an impaired activity."
FT /evidence="ECO:0000269|PubMed:19473327"
FT HELIX 96..99
FT /evidence="ECO:0007829|PDB:6A5E"
FT HELIX 100..102
FT /evidence="ECO:0007829|PDB:6A5E"
SQ SEQUENCE 138 AA; 15049 MW; 074CBDF4DB385523 CRC64;
MRGLSRNSGA AAIFAILLIL AVHNWSVAVS SQSTEFAGDF PPFETECRGT IAECSVSAAL
GDGGDLFYGG GEMGEEFEMD SEINRRILAT RRYISYGALR RNTIPCSRRG ASYYNCRRGA
QANPYSRGCS AITRCRRS
