ID   RLF27_ARATH             Reviewed;         117 AA.
AC   Q9LH43;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Protein RALF-like 27;
DE   Flags: Precursor;
GN   Name=RALFL27; OrderedLocusNames=At3g29780; ORFNames=T26G12.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AP002064; BAB01973.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77610.1; -; Genomic_DNA.
DR   RefSeq; NP_189624.1; NM_113904.3.
DR   AlphaFoldDB; Q9LH43; -.
DR   STRING; 3702.AT3G29780.1; -.
DR   PaxDb; Q9LH43; -.
DR   PRIDE; Q9LH43; -.
DR   EnsemblPlants; AT3G29780.1; AT3G29780.1; AT3G29780.
DR   GeneID; 822684; -.
DR   Gramene; AT3G29780.1; AT3G29780.1; AT3G29780.
DR   KEGG; ath:AT3G29780; -.
DR   Araport; AT3G29780; -.
DR   TAIR; locus:2100582; AT3G29780.
DR   eggNOG; ENOG502SWDV; Eukaryota.
DR   HOGENOM; CLU_169216_0_0_1; -.
DR   InParanoid; Q9LH43; -.
DR   OMA; CIGDKKP; -.
DR   OrthoDB; 1472399at2759; -.
DR   PRO; PR:Q9LH43; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LH43; baseline and differential.
DR   Genevisible; Q9LH43; AT.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   InterPro; IPR039252; RALFL27.
DR   PANTHER; PTHR39112; PTHR39112; 1.
DR   Pfam; PF05498; RALF; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   PROPEP          28..71
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000420324"
FT   CHAIN           72..117
FT                   /note="Protein RALF-like 27"
FT                   /id="PRO_0000420325"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        88..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        107..113
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   117 AA;  12698 MW;  8154E38C31A1E998 CRC64;
     MTKTFFSFSF FFTSSLLLLL AATSATASTG NVTSGLRYDG CAPGDTVGEC ITATVEEEDE
     EGVEAVVRRI LQQRKYLSYK TLQKQPTCDG RIAGNCIGTV NPKGATCTYY QRCKRAA