ID   RLF3_ARATH              Reviewed;          90 AA.
AC   A7REE5; A0MJU9;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Protein RALF-like 3;
DE   Flags: Precursor;
GN   Name=RALFL3; OrderedLocusNames=At1g23147; ORFNames=T26J12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA / MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK27978.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC002311; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002684; AEE30346.1; -; Genomic_DNA.
DR   EMBL; DQ912194; ABI34037.1; -; Genomic_DNA.
DR   EMBL; DQ912248; ABK27978.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; EF182784; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001077586.1; NM_001084117.3.
DR   AlphaFoldDB; A7REE5; -.
DR   SMR; A7REE5; -.
DR   STRING; 3702.AT1G23147.1; -.
DR   PaxDb; A7REE5; -.
DR   PRIDE; A7REE5; -.
DR   EnsemblPlants; AT1G23147.1; AT1G23147.1; AT1G23147.
DR   GeneID; 5007718; -.
DR   Gramene; AT1G23147.1; AT1G23147.1; AT1G23147.
DR   KEGG; ath:AT1G23147; -.
DR   Araport; AT1G23147; -.
DR   TAIR; locus:4010713466; AT1G23147.
DR   HOGENOM; CLU_189400_0_0_1; -.
DR   OMA; IMNAEAR; -.
DR   OrthoDB; 1622483at2759; -.
DR   PhylomeDB; A7REE5; -.
DR   PRO; PR:A7REE5; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; A7REE5; baseline and differential.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..90
FT                   /note="Protein RALF-like 3"
FT                   /id="PRO_0000420293"
FT   DISULFID        59..67
FT                   /evidence="ECO:0000250"
FT   DISULFID        80..86
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   90 AA;  10240 MW;  4BB6D204E2658A32 CRC64;
     MSNLRGTNRF ILVAVLVSFV FLSIMNAEAR KEIGYPKQRF GEDRTNPYEE ITPPLIGGCD
     PKNPQTCLPK QPANPYRRGC LKITRCQRDV