ID   RLF5_ARATH              Reviewed;          89 AA.
AC   A8MQI8;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Protein RALF-like 5;
DE   Flags: Precursor;
GN   Name=RALFL5; OrderedLocusNames=At1g35467; ORFNames=F12A4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   INDUCTION BY SENESCENCE, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- INDUCTION: Accumulates during senescence.
CC       {ECO:0000269|PubMed:12611624}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AC023064; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002684; AEE31797.1; -; Genomic_DNA.
DR   RefSeq; NP_001077666.1; NM_001084197.2.
DR   AlphaFoldDB; A8MQI8; -.
DR   STRING; 3702.AT1G35467.1; -.
DR   PaxDb; A8MQI8; -.
DR   PRIDE; A8MQI8; -.
DR   ProteomicsDB; 228154; -.
DR   EnsemblPlants; AT1G35467.1; AT1G35467.1; AT1G35467.
DR   GeneID; 5007769; -.
DR   Gramene; AT1G35467.1; AT1G35467.1; AT1G35467.
DR   KEGG; ath:AT1G35467; -.
DR   Araport; AT1G35467; -.
DR   TAIR; locus:4010713517; AT1G35467.
DR   HOGENOM; CLU_189400_0_0_1; -.
DR   OMA; KAQVFMF; -.
DR   OrthoDB; 1629905at2759; -.
DR   PhylomeDB; A8MQI8; -.
DR   PRO; PR:A8MQI8; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; A8MQI8; baseline.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..89
FT                   /note="Protein RALF-like 5"
FT                   /id="PRO_0000420296"
FT   DISULFID        39..48
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..67
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   89 AA;  10414 MW;  C36D1EDC7BF968CD CRC64;
     MLKAQVFMFV TVLVFVCVFI NSNDAKRYIE YPPWQKHPCN PRFPTPDCYK RTPANPYRRG
     CTCISRCRRD CGGLSTWKKL LDTILKIPV