RLF8_ARATH
ID RLF8_ARATH Reviewed; 82 AA.
AC Q1ECR9; Q8LBK5;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Protein RALF-like 8;
DE Flags: Precursor;
GN Name=RALFL8; OrderedLocusNames=At1g61563; ORFNames=T25B24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY, INDUCTION BY ABA AND SENESCENCE, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=12611624;
RA Olsen A.N., Mundy J., Skriver K.;
RT "Peptomics, identification of novel cationic Arabidopsis peptides with
RT conserved sequence motifs.";
RL In Silico Biol. 2:441-451(2002).
CC -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC growth, and development. Mediates a rapid alkalinization of
CC extracellular space by mediating a transient increase in the
CC cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC signaling events through a cell surface receptor and a concomitant
CC activation of some intracellular mitogen-activated protein kinases (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and flowers.
CC {ECO:0000269|PubMed:12611624}.
CC -!- INDUCTION: Slightly induced by abscisic acid (ABA) and accumulates
CC during senescence. {ECO:0000269|PubMed:12611624}.
CC -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC family. {ECO:0000305}.
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DR EMBL; AC005850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE33855.1; -; Genomic_DNA.
DR EMBL; BT025636; ABF74697.1; -; mRNA.
DR EMBL; AY087155; AAM64713.1; -; mRNA.
DR RefSeq; NP_564778.1; NM_104837.2.
DR PDB; 6NU4; NMR; -; A=27-82.
DR PDBsum; 6NU4; -.
DR AlphaFoldDB; Q1ECR9; -.
DR SMR; Q1ECR9; -.
DR STRING; 3702.AT1G61563.1; -.
DR PaxDb; Q1ECR9; -.
DR PRIDE; Q1ECR9; -.
DR ProteomicsDB; 228155; -.
DR EnsemblPlants; AT1G61563.1; AT1G61563.1; AT1G61563.
DR GeneID; 842451; -.
DR Gramene; AT1G61563.1; AT1G61563.1; AT1G61563.
DR KEGG; ath:AT1G61563; -.
DR Araport; AT1G61563; -.
DR TAIR; locus:505006195; AT1G61563.
DR eggNOG; ENOG502SW1C; Eukaryota.
DR HOGENOM; CLU_184731_1_0_1; -.
DR InParanoid; Q1ECR9; -.
DR OMA; EARYIDY; -.
DR OrthoDB; 1593801at2759; -.
DR PhylomeDB; Q1ECR9; -.
DR PRO; PR:Q1ECR9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q1ECR9; baseline and differential.
DR Genevisible; Q1ECR9; AT.
DR GO; GO:0048046; C:apoplast; ISS:TAIR.
DR GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR InterPro; IPR008801; RALF.
DR Pfam; PF05498; RALF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Hormone; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..82
FT /note="Protein RALF-like 8"
FT /id="PRO_0000420299"
FT DISULFID 47..55
FT /evidence="ECO:0000250"
FT DISULFID 67..73
FT /evidence="ECO:0000250"
FT CONFLICT 5
FT /note="K -> N (in Ref. 4; AAM64713)"
FT /evidence="ECO:0000305"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:6NU4"
FT TURN 52..54
FT /evidence="ECO:0007829|PDB:6NU4"
SQ SEQUENCE 82 AA; 8906 MW; 929DE1E8C9CB9E36 CRC64;
MGMSKSIKVI LSLALVVFLA LAGTKVEASV RYITYPAIDR GDHAVHCDKA HPNTCKKKQA
NPYRRGCGVL EGCHRETGPK PT
