ID   RLF9_ARATH              Reviewed;          75 AA.
AC   Q3ECL0; Q8LBS8;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Protein RALF-like 9;
DE   Flags: Precursor;
GN   Name=RALFL9; OrderedLocusNames=At1g61566; ORFNames=T25B24;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12611624;
RA   Olsen A.N., Mundy J., Skriver K.;
RT   "Peptomics, identification of novel cationic Arabidopsis peptides with
RT   conserved sequence motifs.";
RL   In Silico Biol. 2:441-451(2002).
CC   -!- FUNCTION: Cell signaling peptide that may regulate plant stress,
CC       growth, and development. Mediates a rapid alkalinization of
CC       extracellular space by mediating a transient increase in the
CC       cytoplasmic Ca(2+) concentration leading to a calcium-dependent
CC       signaling events through a cell surface receptor and a concomitant
CC       activation of some intracellular mitogen-activated protein kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant rapid alkalinization factor (RALF)
CC       family. {ECO:0000305}.
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DR   EMBL; AC005850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002684; AEE33856.1; -; Genomic_DNA.
DR   EMBL; BT026021; ABG25110.1; -; mRNA.
DR   EMBL; AY087014; AAM64575.1; -; mRNA.
DR   RefSeq; NP_564779.1; NM_104838.2.
DR   AlphaFoldDB; Q3ECL0; -.
DR   SMR; Q3ECL0; -.
DR   STRING; 3702.AT1G61566.1; -.
DR   PaxDb; Q3ECL0; -.
DR   PRIDE; Q3ECL0; -.
DR   EnsemblPlants; AT1G61566.1; AT1G61566.1; AT1G61566.
DR   GeneID; 842452; -.
DR   Gramene; AT1G61566.1; AT1G61566.1; AT1G61566.
DR   KEGG; ath:AT1G61566; -.
DR   Araport; AT1G61566; -.
DR   TAIR; locus:505006196; AT1G61566.
DR   eggNOG; ENOG502SW1C; Eukaryota.
DR   HOGENOM; CLU_184731_1_0_1; -.
DR   OMA; CEILERC; -.
DR   OrthoDB; 1645979at2759; -.
DR   PhylomeDB; Q3ECL0; -.
DR   PRO; PR:Q3ECL0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q3ECL0; baseline and differential.
DR   Genevisible; Q3ECL0; AT.
DR   GO; GO:0048046; C:apoplast; ISS:TAIR.
DR   GO; GO:0005179; F:hormone activity; ISS:UniProtKB.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR   GO; GO:0007267; P:cell-cell signaling; ISS:TAIR.
DR   InterPro; IPR008801; RALF.
DR   Pfam; PF05498; RALF; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..75
FT                   /note="Protein RALF-like 9"
FT                   /id="PRO_0000420300"
FT   DISULFID        46..54
FT                   /evidence="ECO:0000250"
FT   DISULFID        66..72
FT                   /evidence="ECO:0000250"
FT   CONFLICT        23
FT                   /note="A -> G (in Ref. 4; AAM64575)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        58
FT                   /note="E -> Q (in Ref. 4; AAM64575)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   75 AA;  8260 MW;  00CB8E2E6C7735CD CRC64;
     MGMSKSIKVI LSLALVVFLA LAATKVEATR YITYPAIDRG DHAVHCDKAH PNTCKKKEAN
     PYQRGCEKIN RCRGG