RLGPB_MOUSE
ID RLGPB_MOUSE Reviewed; 1484 AA.
AC Q8BQZ4; Q80TH5; Q8BQN1;
DT 15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Ral GTPase-activating protein subunit beta;
DE AltName: Full=p170;
GN Name=Ralgapb; Synonyms=Kiaa1219;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1225-1484 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Corpus striatum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 313-1484 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP INTERACTION WITH RALGAPA2, AND TISSUE SPECIFICITY.
RX PubMed=16490346; DOI=10.1016/j.cellsig.2006.01.002;
RA Gridley S., Chavez J.A., Lane W.S., Lienhard G.E.;
RT "Adipocytes contain a novel complex similar to the tuberous sclerosis
RT complex.";
RL Cell. Signal. 18:1626-1632(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-379; SER-421 AND THR-724, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Non-catalytic subunit of the heterodimeric RalGAP1 and
CC RalGAP2 complexes which act as GTPase activators for the Ras-like small
CC GTPases RALA and RALB. {ECO:0000250}.
CC -!- SUBUNIT: Component of the heterodimeric RalGAP1 complex with RALGAPA1
CC and of the heterodimeric RalGAP2 complex with RALGAPA2.
CC Heterodimerization is required for activity (By similarity).
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BQZ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BQZ4-2; Sequence=VSP_009697, VSP_009698;
CC -!- TISSUE SPECIFICITY: Abundantly expressed in testis, pancreas, lung,
CC thymus, brown fat, and white fat. Expressed at lower levels in the
CC brain. {ECO:0000269|PubMed:16490346}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC33137.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK046067; BAC32589.1; -; mRNA.
DR EMBL; AK047718; BAC33137.1; ALT_INIT; mRNA.
DR EMBL; AK122470; BAC65752.1; -; mRNA.
DR AlphaFoldDB; Q8BQZ4; -.
DR STRING; 10090.ENSMUSP00000105111; -.
DR iPTMnet; Q8BQZ4; -.
DR PhosphoSitePlus; Q8BQZ4; -.
DR EPD; Q8BQZ4; -.
DR jPOST; Q8BQZ4; -.
DR MaxQB; Q8BQZ4; -.
DR PaxDb; Q8BQZ4; -.
DR PeptideAtlas; Q8BQZ4; -.
DR PRIDE; Q8BQZ4; -.
DR ProteomicsDB; 299903; -. [Q8BQZ4-1]
DR ProteomicsDB; 299904; -. [Q8BQZ4-2]
DR UCSC; uc008nqg.1; mouse. [Q8BQZ4-2]
DR MGI; MGI:2444531; Ralgapb.
DR eggNOG; KOG3652; Eukaryota.
DR InParanoid; Q8BQZ4; -.
DR PhylomeDB; Q8BQZ4; -.
DR ChiTaRS; Ralgapb; mouse.
DR PRO; PR:Q8BQZ4; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8BQZ4; protein.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005096; F:GTPase activator activity; IGI:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0090630; P:activation of GTPase activity; IGI:MGI.
DR GO; GO:0032484; P:Ral protein signal transduction; IMP:MGI.
DR GO; GO:0060178; P:regulation of exocyst localization; IMP:MGI.
DR GO; GO:0032880; P:regulation of protein localization; IMP:MGI.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.11210; -; 1.
DR InterPro; IPR039930; RALGAPB.
DR InterPro; IPR035974; Rap/Ran-GAP_sf.
DR InterPro; IPR000331; Rap/Ran_GAP_dom.
DR PANTHER; PTHR21344; PTHR21344; 1.
DR SUPFAM; SSF111347; SSF111347; 1.
DR PROSITE; PS50085; RAPGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1484
FT /note="Ral GTPase-activating protein subunit beta"
FT /id="PRO_0000056757"
FT DOMAIN 1138..1382
FT /note="Rap-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00165"
FT REGION 355..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 699..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1297..1325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..385
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..427
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 699..727
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1297..1314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 359
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86X10"
FT MOD_RES 363
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P86410"
FT MOD_RES 379
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 421
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 710
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86X10"
FT MOD_RES 724
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1275
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86X10"
FT VAR_SEQ 473..490
FT /note="ASFIQILLSYKSSIATQA -> GKYAQKHVVIIPFLFLSF (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009697"
FT VAR_SEQ 491..1484
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009698"
FT CONFLICT 1477
FT /note="V -> A (in Ref. 1; BAC33137)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1484 AA; 165200 MW; 24CD07B42542920C CRC64;
MYSEWRSLHL VIQSDQGHTS VLHSYPESVG REVANAVVRP LGQALGTSSV AGSESLLKTD
KEVKWTMEVI CYGLTLPLDG ETVKYCVDVY TDWIMALVLP KDSIPLPIIK EPNLYVQSIL
KHLQNLFVPR QEQGSSQIRL CLQVLRAIQK LARESSIMAR ETWEVLLLFL LQINDILLAP
PTVQGGIAEN LAEKLIGVLF EVWLLACTRC FPTPPYWKTA KEMVANWRHH PAVVEQWSKV
ICALTSRLLR FTYGPSFPPF KVPDEDANLI PPEMDNECIA QTWFRFLHML SNPVDLSNPA
VISSTPKFQE QFLNVSGMPQ ELSQYPCLKH LPQIFFRAMR GISCLVDAFL GISRPRSDSA
PPTPVNRLSM PQSAAVNTTP PHNRRHRAVT VNKATMKTST VTTAHTSKVQ HQASSTSPLS
SPNQTSSEPR PLPAPRRPKV NSILNLFGSW LFDAAFVHCK LHNGINRDNS MTASFIQILL
SYKSSIATQA SMEFRRKGSQ MSTDTMVSNP VFDASEFPDN YEAGRAEACG TLCRIFCSKK
TGEEILPAYL SSVILNSPPL FCCDLKGIDV VVPYFISALE TILPDRELSK FKSYVNPTEL
RRSSINILLS LLPLPHHFGT VRSEVVLEGK FSNDDSSSYD KPITFLSLKL RLVNILIGAL
QTETDPNNTQ MILGAMLNIV QDSALLEAIG CQMEMGGGEN NLKSHSRTNS GISSASGGST
EPTTPDSERP AQALLRDYGS TDSAAGLLIR SIHLVTQRLN SQWRQDMSIS LAALELLSGL
AKVKVMVDSG DRKRAISSVC SYIVYQCSRP APLHSRDLHS MIVAAFQCLC VWLTEHPDML
DEKDCLKEVL EIVELGISGS KSKNSEQEVK YKGDKEPNPA SMRVKDAAEA TLTCIMQLLG
AFPSPSGPAS PCSLVNETTL IKYSRLPTIN KHSFRYFVLD NSVILAMLEQ PLGNEQNDFF
PSVTVLVRGM SGRLAWAQQL CLLPRGAKAN QKLFVPEPRP VPKNDVGFKY SVKHRPFPEE
VDKIPFVKAD LSIPDLHEIV TEELEERHEK LRSGMAQQIA YEMHLEQQSE GELQKRSFPD
PVTDCKPPPP AQEFQTARLF LSHFGFLSLE ALKEPANSRL PPHLIALDST IPGFFDDIGY
LDLLPCRPFD TVFIFYMKPG QKTNQEILKN VESSRNVQPH FLEFLLSLGW SVDVGKHPGW
TGHVSTSWSI NSCDDGEGSE PDEITSSEDV GASIFNGQKK VLYYADALTE IAFVVPSPVE
SLTDSLESNI SDQDSDSNMD LMPGILKQPP LTLELVPNHT DSLNSSQRLS PSSRMKKLPQ
GRPVPPLGPE TRVSVVWVER YDDIENFPLS DLMTEISTGV ETTANSSTSL RSTTLEKEVP
VIFIHPLNTG LFRIKIQGAT GKFNMVIPLV DGMIVSRRAL GFLVRQTVIN ICRRKRLESD
SYSPPHVRRK QKITDIVNKY RNKQLEPEFY TALFQEVGLK NCSS
