RLHA_ECOLI
ID RLHA_ECOLI Reviewed; 653 AA.
AC P76104; P76865; P76867; P76868;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=23S rRNA 5-hydroxycytidine C2501 synthase {ECO:0000305};
DE AltName: Full=Large subunit ribosomal RNA hydroxylation A {ECO:0000303|PubMed:29069499};
GN Name=rlhA {ECO:0000303|PubMed:29069499}; Synonyms=ydcP;
GN OrderedLocusNames=b1435, JW1431;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, ACTIVITY REGULATION, SUBUNIT, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF GLU-161; HIS-165; CYS-169; GLN-175; CYS-176; CYS-580 AND
RP CYS-611.
RX PubMed=29069499; DOI=10.1093/nar/gkx969;
RA Kimura S., Sakai Y., Ishiguro K., Suzuki T.;
RT "Biogenesis and iron-dependency of ribosomal RNA hydroxylation.";
RL Nucleic Acids Res. 45:12974-12986(2017).
CC -!- FUNCTION: Responsible for the formation of the 5-hydroxycytidine
CC modification at the C2501 position (ho5C2501) of 23S rRNA. May be a Fe-
CC S protein that catalyzes ho5C2501 formation using prephenate as a
CC hydroxyl group donor. {ECO:0000269|PubMed:29069499}.
CC -!- ACTIVITY REGULATION: Iron-sulfur clusters and prephenate are required
CC for ho5C2501 formation. {ECO:0000269|PubMed:29069499}.
CC -!- SUBUNIT: Interacts with precursors of the 50S ribosomal subunit.
CC {ECO:0000269|PubMed:29069499}.
CC -!- INTERACTION:
CC P76104; P05824: recN; NbExp=3; IntAct=EBI-556974, EBI-548098;
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene results in complete loss of
CC the ho5C2501 modification. {ECO:0000269|PubMed:29069499}.
CC -!- SIMILARITY: Belongs to the peptidase U32 family. {ECO:0000305}.
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DR EMBL; U00096; AAC74517.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15068.1; -; Genomic_DNA.
DR RefSeq; NP_415952.2; NC_000913.3.
DR RefSeq; WP_001303492.1; NZ_SSZK01000021.1.
DR AlphaFoldDB; P76104; -.
DR SMR; P76104; -.
DR BioGRID; 4263353; 39.
DR BioGRID; 850355; 1.
DR DIP; DIP-11650N; -.
DR IntAct; P76104; 50.
DR STRING; 511145.b1435; -.
DR MEROPS; U32.003; -.
DR jPOST; P76104; -.
DR PaxDb; P76104; -.
DR PRIDE; P76104; -.
DR EnsemblBacteria; AAC74517; AAC74517; b1435.
DR EnsemblBacteria; BAA15068; BAA15068; BAA15068.
DR GeneID; 945993; -.
DR KEGG; ecj:JW1431; -.
DR KEGG; eco:b1435; -.
DR PATRIC; fig|1411691.4.peg.834; -.
DR EchoBASE; EB3522; -.
DR eggNOG; COG0826; Bacteria.
DR HOGENOM; CLU_011540_5_0_6; -.
DR InParanoid; P76104; -.
DR OMA; MKDNNQS; -.
DR PhylomeDB; P76104; -.
DR BioCyc; EcoCyc:G6746-MON; -.
DR BioCyc; MetaCyc:G6746-MON; -.
DR PRO; PR:P76104; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0000154; P:rRNA modification; IMP:EcoCyc.
DR InterPro; IPR020988; Pept_U32_collagenase.
DR InterPro; IPR001539; Peptidase_U32.
DR Pfam; PF12392; DUF3656; 1.
DR Pfam; PF01136; Peptidase_U32; 1.
DR PROSITE; PS01276; PEPTIDASE_U32; 1.
PE 1: Evidence at protein level;
KW Reference proteome; rRNA processing.
FT CHAIN 1..653
FT /note="23S rRNA 5-hydroxycytidine C2501 synthase"
FT /id="PRO_0000028524"
FT MUTAGEN 161
FT /note="E->A: Strong decrease in activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 165
FT /note="H->A: No change in activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 169
FT /note="C->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 175
FT /note="Q->A: No change in activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 176
FT /note="C->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 580
FT /note="C->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:29069499"
FT MUTAGEN 611
FT /note="C->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:29069499"
SQ SEQUENCE 653 AA; 72702 MW; 5875B4E9C2F4FC82 CRC64;
MTVSSHRLEL LSPARDAAIA REAILHGADA VYIGGPGFGA RHNASNSLKD IAELVPFAHR
YGAKIFVTLN TILHDDELEP AQRLITDLYQ TGVDALIVQD MGILELDIPP IELHASTQCD
IRTVEKAKFL SDVGFTQIVL ARELNLDQIR AIHQATDATI EFFIHGALCV AYSGQCYISH
AQTGRSANRG DCSQACRLPY TLKDDQGRVV SYEKHLLSMK DNDQTANLGA LIDAGVRSFK
IEGRYKDMSY VKNITAHYRQ MLDAIIEERG DLARASSGRT EHFFVPSTEK TFHRGSTDYF
VNARKGDIGA FDSPKFIGLP VGEVVKVAKD HLDVAVTEPL ANGDGLNVLI KREVVGFRAN
TVEKTGENQY RVWPNEMPAD LHKIRPHHPL NRNLDHNWQQ ALTKTSSERR VAVDIELGGW
QEQLILTLTS EEGVSITHTL DGQFDEANNA EKAMNNLKDG LAKLGQTLYY ARDVQINLPG
ALFVPNSLLN QFRREAADML DAARLASYQR GSRKPVADPA PVYPQTHLSF LANVYNQKAR
EFYHRYGVQL IDAAYEAHEE KGEVPVMITK HCLRFAFNLC PKQAKGNIKS WKATPMQLVN
GDEVLTLKFD CRPCEMHVIG KIKNHILKMP LPGSVVASVS PDELLKTLPK RKG
