RLM1A_ARATH
ID RLM1A_ARATH Reviewed; 997 AA.
AC F4I594; Q9SH57;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Disease resistance protein RML1A {ECO:0000305};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein RESISTANCE TO LEPTOSPHAERIA MACULANS 1A {ECO:0000303|PubMed:16623885};
GN Name=RLM1A {ECO:0000303|PubMed:16623885};
GN OrderedLocusNames=At1g64070 {ECO:0000312|Araport:AT1G64070};
GN ORFNames=F22C12.17 {ECO:0000312|EMBL:AAF24575.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16623885; DOI=10.1111/j.1365-313x.2006.02688.x;
RA Staal J., Kaliff M., Bohman S., Dixelius C.;
RT "Transgressive segregation reveals two Arabidopsis TIR-NB-LRR resistance
RT genes effective against Leptosphaeria maculans, causal agent of blackleg
RT disease.";
RL Plant J. 46:218-230(2006).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19220763; DOI=10.1111/j.1469-8137.2009.02763.x;
RA Persson M., Staal J., Oide S., Dixelius C.;
RT "Layers of defense responses to Leptosphaeria maculans below the RLM1- and
RT camalexin-dependent resistances.";
RL New Phytol. 182:470-482(2009).
CC -!- FUNCTION: TIR-NB-LRR receptor-like protein that confers resistance to
CC the pathogen Leptosphaeria maculans (blackleg disease).
CC {ECO:0000269|PubMed:16623885, ECO:0000269|PubMed:19220763}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants are susceptible to the pathogen
CC Leptosphaeria maculans. {ECO:0000269|PubMed:16623885,
CC ECO:0000269|PubMed:19220763}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF24575.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g64065 and At1g64070.; Evidence={ECO:0000305};
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DR EMBL; AC007764; AAF24575.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34188.1; -; Genomic_DNA.
DR RefSeq; NP_176590.2; NM_105080.3.
DR AlphaFoldDB; F4I594; -.
DR SMR; F4I594; -.
DR STRING; 3702.AT1G64070.1; -.
DR iPTMnet; F4I594; -.
DR PaxDb; F4I594; -.
DR PRIDE; F4I594; -.
DR ProteomicsDB; 228171; -.
DR EnsemblPlants; AT1G64070.1; AT1G64070.1; AT1G64070.
DR GeneID; 842711; -.
DR Gramene; AT1G64070.1; AT1G64070.1; AT1G64070.
DR KEGG; ath:AT1G64070; -.
DR Araport; AT1G64070; -.
DR TAIR; locus:2024588; AT1G64070.
DR HOGENOM; CLU_001561_0_1_1; -.
DR InParanoid; F4I594; -.
DR OMA; NWDDEAT; -.
DR OrthoDB; 94736at2759; -.
DR PRO; PR:F4I594; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I594; baseline and differential.
DR Genevisible; F4I594; AT.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 3: Inferred from homology;
KW ATP-binding; Hydrolase; Leucine-rich repeat; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..997
FT /note="Disease resistance protein RML1A"
FT /id="PRO_0000433375"
FT DOMAIN 12..176
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 191..447
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 194..218
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 534..557
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 600..623
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 624..647
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 649..670
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 671..693
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 694..714
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 715..737
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 758..781
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 783..808
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT ACT_SITE 87
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
SQ SEQUENCE 997 AA; 113174 MW; 7EC405DB3BDE7D03 CRC64;
MASSSSSASR TWRYRVFTSF HGSDVRTSFL SHFRKQFNNN GITMFDDQRI LRGETISPAL
TQAIRESRIS IVLLSKNYAS SGWCLDELLE ILKCKDDMGQ IVMTVFYGVD PSDVRKQTGE
FGIAFNETCA CRTEEERQKW SQALNYVGNI AGEHLLNWDN EAKMIEKIAR DVSEKLNVTP
CRDFDGMVGI EAHLRKIQSL LDLDNDEVKM VAISGPAGIG KSTIGRALHS LLSNRFHHTC
FVDNLRGSHP IGLDEYGLKL RLQEQLLSKI LNQDGSRICH LGAIKERLCD MKVFIILDDV
NDVKQLEALA NESNWFGPGS RIIVTTENKE LLKQHGINNT YYVGFPSDEE AIKILCRYAF
RQSSSRHGFK KLTRSVTELC GKLPLGLRVV GSSLHGKNEE EWEYVIRRLE TIIDRDIEQV
LRVGYESLHE NEQSLFLHIA IFFNYEDGDL VKAMLAENDL DIEHELNILV NKSLIYISTD
GRIRMHKLLQ LVGRQANQRE EPWKRRILID AQEICHVLEN DIGTGAVSGI LFDTSGINEV
SISNKALRRM CNLRFLSVYK TKHDGYNRMD IPEDMEFPPR LRLLHWDAYP SKCLPLKFRA
ENLVELDMKD SRLEYLWPGT QLLTKLKKLN LEGSYNLKEL PDLSNATNLE MLDLSVCLAL
AELPSSIKNL HKLDVIYMDL CESLHMIPTN INLASLETMY MTGCPQLKTF PAFSTKIKRL
YLVRTGVEEV PASITHCSRL LKIDLSGSRN LKSITHLPSS LQTLDLSSTD IEMIADSCIK
DLQRLDHLRL CRCRKLKSLP ELPASLRLLT AEDCESLERV TYPLNTPTGQ LNFTNCLKLG
EEAQRVIIQQ SLVKHACFPG SVMPSEFNHR ARGNSLKILV KSSASFAFKA CVLISPRQLQ
CERNQRRVKI RCRVTDGRGR FVGSKVVSLE HPNHSTGIRT KHLCFFNGVL TEVSCDALCF
VFKISAYNPL DNYEISECAV QILTNEPERR SCDGGSE
