RLM1B_ARATH
ID RLM1B_ARATH Reviewed; 1017 AA.
AC Q9CAK1; Q7FKS0;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Disease resistance protein RML1B {ECO:0000305};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein RESISTANCE TO LEPTOSPHAERIA MACULANS 1B {ECO:0000303|PubMed:16623885};
GN Name=RLM1B {ECO:0000303|PubMed:16623885};
GN OrderedLocusNames=At1g63880 {ECO:0000312|Araport:AT1G63880};
GN ORFNames=T12P18.10 {ECO:0000312|EMBL:AAG52448.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16623885; DOI=10.1111/j.1365-313x.2006.02688.x;
RA Staal J., Kaliff M., Bohman S., Dixelius C.;
RT "Transgressive segregation reveals two Arabidopsis TIR-NB-LRR resistance
RT genes effective against Leptosphaeria maculans, causal agent of blackleg
RT disease.";
RL Plant J. 46:218-230(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19220763; DOI=10.1111/j.1469-8137.2009.02763.x;
RA Persson M., Staal J., Oide S., Dixelius C.;
RT "Layers of defense responses to Leptosphaeria maculans below the RLM1- and
RT camalexin-dependent resistances.";
RL New Phytol. 182:470-482(2009).
CC -!- FUNCTION: TIR-NB-LRR receptor-like protein that confers resistance to
CC the pathogen Leptosphaeria maculans (blackleg disease).
CC {ECO:0000269|PubMed:16623885, ECO:0000269|PubMed:19220763}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants are susceptible to the pathogen
CC Leptosphaeria maculans. {ECO:0000269|PubMed:16623885,
CC ECO:0000269|PubMed:19220763}.
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DR EMBL; AC010852; AAG52448.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34163.1; -; Genomic_DNA.
DR EMBL; AK119065; BAC43641.2; -; mRNA.
DR PIR; H96663; H96663.
DR RefSeq; NP_176572.1; NM_105062.3.
DR AlphaFoldDB; Q9CAK1; -.
DR SMR; Q9CAK1; -.
DR STRING; 3702.AT1G63880.1; -.
DR PaxDb; Q9CAK1; -.
DR PRIDE; Q9CAK1; -.
DR ProteomicsDB; 228135; -.
DR EnsemblPlants; AT1G63880.1; AT1G63880.1; AT1G63880.
DR GeneID; 842692; -.
DR Gramene; AT1G63880.1; AT1G63880.1; AT1G63880.
DR KEGG; ath:AT1G63880; -.
DR Araport; AT1G63880; -.
DR TAIR; locus:2195468; AT1G63880.
DR HOGENOM; CLU_001561_0_1_1; -.
DR InParanoid; Q9CAK1; -.
DR OrthoDB; 258604at2759; -.
DR PhylomeDB; Q9CAK1; -.
DR PRO; PR:Q9CAK1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAK1; baseline and differential.
DR Genevisible; Q9CAK1; AT.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Hydrolase; Leucine-rich repeat; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..1017
FT /note="Disease resistance protein RML1B"
FT /id="PRO_0000433376"
FT DOMAIN 12..176
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 191..447
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 539..562
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 583..605
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 606..628
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 629..652
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 654..675
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 676..698
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 699..724
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 738..760
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 761..782
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 784..809
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT ACT_SITE 87
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT CONFLICT 312
FT /note="E -> G (in Ref. 3; BAC43641)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1017 AA; 115415 MW; 9C1AF3178F70F7D4 CRC64;
MASPSSFSSQ NYKFNVFASF HGPDVRKTLL SHIRLQFNRN GITMFDDQKI VRSATIGPSL
VEAIKESRIS IVILSKKYAS SSWCLDELVE ILECKKAMGQ IVMTIFYGVD PSDVRKQIGK
FGIAFNETCA RKTEEERQKW SKALNQVSNI AGEDFLRWDN EAIMIEKIAR DVLDKLNATP
SRDFDGMVGI EAHLREIKSL LDLDNVEVKI VAIAGPAGIG KTTIARALYG LLSKRFQLSC
FVDNLRGSYH SGFDEYGFKL HLQEQFLSKV LNQSGMRICH LGAIKENLSD QRVLIILDDV
NKLKQLEALA NETTWFGPGS RIVVTTENKE LLQQHGINNT YHVGFPSDED ALKILCSYAF
KQTSPRHGFE ELSESVTKLC GKLPLGLCVV GSSLRGKKED EWEDVVTRLE TILDQDIEDV
LRVGYESLDE NAQTLFLHIA IFFNKEDGDL VKTMFAESDL DVKYGLKILE NRSLIKMKIF
SNGDTKIVMH RLLQQMGKRA IQKQEPWERQ ILIDAREICH VLEHAKGTGW NVHGMSFDIS
RISEVSIRKK AFKRMPNLQF LKVYKSKDDG NNRMHVPEEM DFPCLLRLLD WKAYPSKSLP
PTFNPEHLVE LNMHSSQLEY LWQGTQPLKN LKKMDLSQSK NLKQLPDLSN ATNLEYLYLM
GCESLIEIPS SISHLHKLEM LATVGCINLE VIPAHMNLES LQTVYLGGCS RLRNIPVMST
NIRYLFITNT AVEGVPLCPG LKTLDVSGSR NFKGLLTHLP TSLTTLNLCY TDIERIPDCF
KSLHQLKGVN LRGCRRLASL PELPRSLLTL VADDCESLET VFCPLNTLKA SFSFANCFKL
DREARRAIIQ QSFFMGKAVL PGREVPAVFD HRAKGYSLTI RPDGNPYTSF VFCVVVSRNQ
KSDKTIPPSL LWRRIIAQDE GYPVEVWNRI GDVFKYRTEH LLIFHFDFLE FDNRDIVFEF
SSESHDFDII ECGAKVLAEK SIKESYESGS DQAFEDDVVF EPSKAFGDEK YGDCCIL
