RLM3_ARATH
ID RLM3_ARATH Reviewed; 796 AA.
AC Q9FT77; F4JNC3; Q0WKX9; Q3EA02; Q682W8; Q9SUK2;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 142.
DE RecName: Full=Disease resistance protein RLM3 {ECO:0000305};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein RESISTANCE TO LEPTOSPHAERIA MACULANS 3 {ECO:0000303|PubMed:18397376};
GN Name=RLM3 {ECO:0000303|PubMed:18397376};
GN OrderedLocusNames=At4g16990 {ECO:0000312|Araport:AT4G16990};
GN ORFNames=dl4525w {ECO:0000312|EMBL:CAB46050.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Acarkan A., Schmidt R.H.;
RT "Comparative analysis of the Arabidopsis thaliana and Capsella rubella
RT genomes.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|Proteomes:UP000006548}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=18397376; DOI=10.1111/j.1365-313x.2008.03503.x;
RA Staal J., Kaliff M., Dewaele E., Persson M., Dixelius C.;
RT "RLM3, a TIR domain encoding gene involved in broad-range immunity of
RT Arabidopsis to necrotrophic fungal pathogens.";
RL Plant J. 55:188-200(2008).
CC -!- FUNCTION: TIR-NB-LRR receptor-like protein that confers resistance to
CC the pathogens Leptosphaeria maculans (blackleg disease), Botrytis
CC cinerea, Alternaria brassicicola and Alternaria brassicae. Required for
CC efficient callose deposition downstream of RLM1 during infection with
CC L.maculans. {ECO:0000269|PubMed:18397376}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9FT77-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FT77-2; Sequence=VSP_057759, VSP_057760, VSP_057761;
CC Name=3;
CC IsoId=Q9FT77-3; Sequence=VSP_057758;
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB46050.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80970.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ299417; CAC14089.1; -; mRNA.
DR EMBL; Z97342; CAB46050.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161545; CAB80970.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83831.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83832.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83833.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM68095.1; -; Genomic_DNA.
DR EMBL; AK175249; BAD43012.1; -; mRNA.
DR EMBL; AK230430; BAF02228.1; -; mRNA.
DR PIR; F85189; F85189.
DR RefSeq; NP_001319972.1; NM_001341182.1. [Q9FT77-1]
DR RefSeq; NP_193432.4; NM_117803.4. [Q9FT77-2]
DR RefSeq; NP_849399.1; NM_179068.2. [Q9FT77-1]
DR RefSeq; NP_849400.2; NM_179069.2. [Q9FT77-3]
DR AlphaFoldDB; Q9FT77; -.
DR SMR; Q9FT77; -.
DR STRING; 3702.AT4G16990.2; -.
DR iPTMnet; Q9FT77; -.
DR PaxDb; Q9FT77; -.
DR PRIDE; Q9FT77; -.
DR ProteomicsDB; 228096; -. [Q9FT77-1]
DR EnsemblPlants; AT4G16990.1; AT4G16990.1; AT4G16990. [Q9FT77-2]
DR EnsemblPlants; AT4G16990.13; AT4G16990.13; AT4G16990. [Q9FT77-1]
DR EnsemblPlants; AT4G16990.2; AT4G16990.2; AT4G16990. [Q9FT77-1]
DR EnsemblPlants; AT4G16990.3; AT4G16990.3; AT4G16990. [Q9FT77-3]
DR GeneID; 827407; -.
DR Gramene; AT4G16990.1; AT4G16990.1; AT4G16990. [Q9FT77-2]
DR Gramene; AT4G16990.13; AT4G16990.13; AT4G16990. [Q9FT77-1]
DR Gramene; AT4G16990.2; AT4G16990.2; AT4G16990. [Q9FT77-1]
DR Gramene; AT4G16990.3; AT4G16990.3; AT4G16990. [Q9FT77-3]
DR KEGG; ath:AT4G16990; -.
DR Araport; AT4G16990; -.
DR TAIR; locus:2130319; AT4G16990.
DR HOGENOM; CLU_353156_0_0_1; -.
DR InParanoid; Q9FT77; -.
DR OMA; WYENCER; -.
DR PhylomeDB; Q9FT77; -.
DR PRO; PR:Q9FT77; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9FT77; baseline and differential.
DR Genevisible; Q9FT77; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:2000071; P:regulation of defense response by callose deposition; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR013591; Brevis_radix_dom.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF08381; BRX; 3.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51514; BRX; 3.
DR PROSITE; PS50104; TIR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Hydrolase; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..796
FT /note="Disease resistance protein RLM3"
FT /id="PRO_0000433381"
FT DOMAIN 7..171
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 185..413
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT DOMAIN 434..486
FT /note="BRX 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00847"
FT DOMAIN 538..594
FT /note="BRX 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00847"
FT DOMAIN 719..772
FT /note="BRX 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00847"
FT REGION 502..537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 607..636
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..531
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT VAR_SEQ 1..158
FT /note="Missing (in isoform 3)"
FT /id="VSP_057758"
FT VAR_SEQ 567
FT /note="Missing (in isoform 2)"
FT /id="VSP_057759"
FT VAR_SEQ 667..671
FT /note="LTDVN -> KGSDI (in isoform 2)"
FT /id="VSP_057760"
FT VAR_SEQ 672..796
FT /note="Missing (in isoform 2)"
FT /id="VSP_057761"
FT CONFLICT 601
FT /note="R -> G (in Ref. 6; BAF02228)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 796 AA; 91154 MW; 30E0AF0590B6BA94 CRC64;
MKSSSSQSYD VFPNFRGEDV RHSLVSHLRK ELDRKFINTF NDNRIERSRK ITPELLLAIE
NSRISLVVFS KNYASSTWCL DELVKIQECY EKLDQMVIPI FYKVDPSHVR KQTGEFGMVF
GETCKGRTEN EKRKWMRALA EVAHLAGEDL RNWRSEAEML ENIAKDVSNK LFPPSNNFSD
FVGIEAHIEA LISMLRFDSK KARMIGICGP SETGKTTIGR ALYSRLKSDF HHRAFVAYKR
KIRSDYDQKL YWEEQFLSEI LCQKDIKIEE CGAVEQRLKH TKVLIVLDDV DDIELLKTLV
GRIRWFGSES KIVVITQKRE LLKAHNIAHV YEVGFPSEEL AHQMFCRYAF GKNSPPHGFN
ELADEAAKIA GNRPKALKYV GSSFRRLDKE QWVKMLSEFR SNGNKLKISY DELDGKGQDY
VACLTNGSNS QVKAEWIHLA LGVSILLNIR SDGTTILKHL SYNRSMAQQA KIWWYENLER
VCKKYNICGI DSSTDGGGST YGQCSNSQFQ RNMDASPGGN KTSNQSTKDS PRASQVEKEK
IEYCEPHVYI TPAIFSDGTR APKYVESSSR RVTQVHHAKT WWPENCEKVY ENHNNIYGID
RSIDGGDKFE GKSKVSDGGL DGKDQGSMYG QSSNSELQIN MDADNRRCEP VSEMLFKNYN
VCSPNGLTDV NCSNPQSQRK LDASLKKDKI VHEWIRTGSG FFFDFQGPKS IVSAAQVDEK
NFEYCEQGVY ITLGILSGGI IVLKHLEFSR RMAQQAKVWW SENWIKVYQE HNICGIDKSF
DGRFDDRRVI RQLRPN
