RLMA_ASPFC
ID RLMA_ASPFC Reviewed; 600 AA.
AC B0XYE0;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=MADS-box transcription factor rlmA {ECO:0000303|PubMed:27393422};
GN Name=rlmA {ECO:0000303|PubMed:27393422}; ORFNames=AFUB_040580;
OS Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) (Aspergillus
OS fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=451804;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CEA10 / CBS 144.89 / FGSC A1163;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=27393422; DOI=10.1111/mmi.13462;
RA Valiante V., Baldin C., Hortschansky P., Jain R., Thywissen A.,
RA Strassburger M., Shelest E., Heinekamp T., Brakhage A.A.;
RT "The Aspergillus fumigatus conidial melanin production is regulated by the
RT bifunctional bHLH DevR and MADS-box RlmA transcription factors.";
RL Mol. Microbiol. 102:321-335(2016).
RN [3]
RP INDUCTION.
RX PubMed=30914505; DOI=10.1128/mbio.00215-19;
RA Manfiolli A.O., Siqueira F.S., Dos Reis T.F., Van Dijck P., Schrevens S.,
RA Hoefgen S., Foege M., Strassburger M., de Assis L.J., Heinekamp T.,
RA Rocha M.C., Janevska S., Brakhage A.A., Malavazi I., Goldman G.H.,
RA Valiante V.;
RT "Mitogen-activated protein kinase cross-talk interaction modulates the
RT production of melanins in Aspergillus fumigatus.";
RL MBio 10:0-0(2019).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=33705521; DOI=10.1093/genetics/iyab036;
RA Rocha M.C., Fabri J.H.T.M., da Silva L.P., Angolini C.F.F., Bertolini M.C.,
RA da Cunha A.F., Valiante V., Goldman G.H., Fill T.P., Malavazi I.;
RT "Transcriptional control of the production of Aspergillus fumigatus
RT conidia-borne secondary metabolite fumiquinazoline C important for
RT phagocytosis protection.";
RL Genetics 0:0-0(2021).
CC -!- FUNCTION: MADS-box transcription factor involved in the activation of
CC the dihydroxynaphthalene (DHN)-melanin pathway and the fumiquinazoline
CC C cluster during conidiogenesis (PubMed:27393422, PubMed:33705521).
CC Specifically recognizes a DNA binding site (poly A/T stretch)
CC identified in the promoter sequence of the gene coding for the
CC polyketide synthase pksP and of the fumiquinazoline C cluster (fmq)
CC promoters (PubMed:27393422, PubMed:33705521).
CC {ECO:0000269|PubMed:27393422, ECO:0000269|PubMed:33705521}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27393422}.
CC -!- INDUCTION: Expression is inceased two fold when the MAPK mpkB is
CC deleted. {ECO:0000269|PubMed:30914505}.
CC -!- DISRUPTION PHENOTYPE: Affects sporulation and reduces the expression of
CC the DHN-melanin gene cluster (PubMed:27393422). Decreases also strongly
CC the production of fumiquinazoline C (PubMed:33705521).
CC {ECO:0000269|PubMed:27393422, ECO:0000269|PubMed:33705521}.
CC -!- SIMILARITY: Belongs to the MEF2 family. {ECO:0000305}.
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DR EMBL; DS499596; EDP52886.1; -; Genomic_DNA.
DR EnsemblFungi; EDP52886; EDP52886; AFUB_040580.
DR VEuPathDB; FungiDB:AFUB_040580; -.
DR HOGENOM; CLU_024080_1_0_1; -.
DR PhylomeDB; B0XYE0; -.
DR Proteomes; UP000001699; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Transcription; Transcription regulation.
FT CHAIN 1..600
FT /note="MADS-box transcription factor rlmA"
FT /id="PRO_0000454892"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT REGION 71..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 542..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..247
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 367..381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 400..472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..518
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 573..587
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 600 AA; 64397 MW; 8A52DA433F4169CF CRC64;
MGRRKIEIKA IKDDRNRSVT FLKRKGGLFK KAHELAVLCS VDVAVIIFGH NKKLYEFSSC
DMRETLGRYQ YYGPPHEHKG PEDFNGKRDD DDDEDETTPA PEEMQPTTQN PPAVVPAHIP
SHPGFQHVNH APSASPPISN GIPFDPRHGT PQPQGASRPS SRNHLRRVSS NLGPQQHHGT
PPPPPQNGFA YIPNPSMYHP NANPNIAQQP RPPQFAHYGP QQPLPPHAIP PHPMPQPVPP
HHQAPQHLPQ HPHPLAQQTP AMGLSQPPHA SIPQVAQPFL PEQGRNSMPP AFPTEQSQPP
RPVSLPDVSS ADQMVGPLKV ETSPSPPHQR SLSSKSRSIF TPIDDRGSVL ARHFGLGPPT
CESPRTESAD VKAEAKQNDS KEIKPPAQPV APPPPPRTTA DAARSQSAPD IKPPPRTNSG
QLPSKRPQLK VQIPSENSDR GSATADSSSS TGNQTVTPAK ANPDTNHSGV VLPPPSPSAG
AILSAGATGP PNPFARPPPP GTASQNSNAY NSNNNIETPI SALPSRFVSD ALLPSPSSFF
PEWGFGRSGP DTNMLPSPLT FPTPAVQTGP GFAREDEQEK KRKSPDSGPS IEGTAKKSKT
