AB12B_ARATH
ID AB12B_ARATH Reviewed; 1273 AA.
AC Q9FWX8;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=ABC transporter B family member 12;
DE Short=ABC transporter ABCB.12;
DE Short=AtABCB12;
DE AltName: Full=Multidrug resistance protein 16;
DE AltName: Full=P-glycoprotein 12;
GN Name=ABCB12; Synonyms=MDR16, PGP12; OrderedLocusNames=At1g02530;
GN ORFNames=T14P4.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family.
CC Multidrug resistance exporter (TC 3.A.1.201) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG10627.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AK228647; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC022521; AAG10627.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE27441.1; -; Genomic_DNA.
DR EMBL; AK228647; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; F86155; F86155.
DR RefSeq; NP_171754.1; NM_100134.4.
DR AlphaFoldDB; Q9FWX8; -.
DR SMR; Q9FWX8; -.
DR BioGRID; 24517; 1.
DR STRING; 3702.AT1G02530.1; -.
DR PaxDb; Q9FWX8; -.
DR PRIDE; Q9FWX8; -.
DR ProteomicsDB; 245140; -.
DR EnsemblPlants; AT1G02530.1; AT1G02530.1; AT1G02530.
DR GeneID; 839282; -.
DR Gramene; AT1G02530.1; AT1G02530.1; AT1G02530.
DR KEGG; ath:AT1G02530; -.
DR Araport; AT1G02530; -.
DR TAIR; locus:2196135; AT1G02530.
DR eggNOG; KOG0055; Eukaryota.
DR HOGENOM; CLU_000604_17_2_1; -.
DR InParanoid; Q9FWX8; -.
DR OMA; FGYMQIS; -.
DR OrthoDB; 186078at2759; -.
DR PhylomeDB; Q9FWX8; -.
DR BioCyc; ARA:AT1G02530-MON; -.
DR PRO; PR:Q9FWX8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FWX8; baseline and differential.
DR Genevisible; Q9FWX8; AT.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1560.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039421; Type_1_exporter.
DR PANTHER; PTHR24221; PTHR24221; 1.
DR Pfam; PF00664; ABC_membrane; 2.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF90123; SSF90123; 2.
DR PROSITE; PS50929; ABC_TM1F; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 2: Evidence at transcript level;
KW ATP-binding; Glycoprotein; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1273
FT /note="ABC transporter B family member 12"
FT /id="PRO_0000227927"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 706..726
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 746..766
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 840..860
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 927..947
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 961..981
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 45..333
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 368..604
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 705..992
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 1027..1266
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 403..410
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 1062..1069
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CARBOHYD 470
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 555
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 631
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 648
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 801
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1217
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1247
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 279
FT /note="I -> T (in Ref. 3; AK228647)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1273 AA; 136774 MW; 1CCA06FB3D472241 CRC64;
MNRDGAGEGD SVSHEHSTSK TDEKAKTVPL YKLFAFADSF DVFLMICGSL GAIGNGVCLP
LMTLLFGDLI DSFGKNQNNK DIVDVVSKVC LKFVYLGLGR LGAAFLQVAC WMITGERQAA
KIRSNYLKTI LRQDIGFFDV ETNTGEVVGR MSGDTVHIQD AMGEKVGKFI QLVSTFVGGF
ALAFAKGWLL TLVMLTSIPF LAMAGAAMAL LVTRASSRGQ AAYAKAATVV EQTIGSIRTV
ASFTGEKQAI NSYKKYITSA YKSSIQQGFS TGLGLGVMIY VFFSSYALAI WFGGKMILEK
GYTGGSVINV IIIVVAGSMS LGQTSPCVTA FAAGQAAAYK MFETIKRKPL IDAYDVNGKV
LGDIRGDIEL KDVHFSYPAR PDEEIFDGFS LFIPSGATAA LVGESGSGKS TVINLIERFY
DPKAGEVLID GINLKEFQLK WIRSKIGLVC QEPVLFSSSI MENIAYGKEN ATLQEIKVAT
ELANAAKFIN NLPQGLDTKV GEHGTQLSGG QKQRIAIARA ILKDPRVLLL DEATSALDTE
SERVVQEALD RVMVNRTTVV VAHRLSTVRN ADMIAVIHSG KMVEKGSHSE LLKDSVGAYS
QLIRCQEINK GHDAKPSDMA SGSSFRNSNL NISREGSVIS GGTSSFGNSS RHHSLNVLGL
FAGLDLGSGS QRVGQEETGT TSQEPLRKVS LTRIAALNKP EIPVLLLGTV VAAINGAIFP
LFGILISRVI EAFFKPADQL KKDSRFWAII FVALGVTSLI VSPSQMYLFA VAGGKLIRRI
QSMCFEKAVH MEVSWFDEPE NSSGTMGARL STDAALIRAL VGDALSLAVQ NAASAASGLI
IAFTASWELA LIILVMLPLI GINGFLQVKF MKGFSADAKS KYEEASQVAN DAVGSIRTVA
SFCAEEKVMQ MYNKQCEGPI KDGVKQGFIS GLGFGFSFFI LFCVYATSFY AAARLVEDGK
TTFIDVFQVF FALTMAAIGI SQSSTFAPDS SKAKVAAASI FAIIDRKSKI DSSDETGTVL
ENVKGDIELR HLSFTYPARP GIQIFRDLCL TIRAGKTVAL VGESGSGKST VISLLQRFYD
PDSGQITLDG VELKKLQLKW LRQQMGLVGQ EPVLFNDTIR ANIAYGKGSE EAATESEIIA
AAELANAHKF ISSIQQGYDT VVGEKGIQLS GGQKQRVAIA RAIVKEPKIL LLDEATSALD
AESERLVQDA LDRVIVNRTT VVVAHRLSTI KNADVIAIVK NGVIAENGTH ETLIKIDGGV
YASLVQLHMT ASN
