RLMF_ECOLI
ID RLMF_ECOLI Reviewed; 308 AA.
AC P75782; Q9R3Q9;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase F;
DE EC=2.1.1.181 {ECO:0000269|PubMed:18021804};
DE AltName: Full=23S rRNA mA1618 methyltransferase;
DE AltName: Full=rRNA adenine N-6-methyltransferase;
GN Name=rlmF; Synonyms=ybiN; OrderedLocusNames=b0807, JW5107;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=18021804; DOI=10.1016/j.jmb.2007.10.051;
RA Sergiev P.V., Serebryakova M.V., Bogdanov A.A., Dontsova O.A.;
RT "The ybiN gene of Escherichia coli encodes adenine-N6 methyltransferase
RT specific for modification of A1618 of 23 S ribosomal RNA, a methylated
RT residue located close to the ribosomal exit tunnel.";
RL J. Mol. Biol. 375:291-300(2008).
CC -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC rRNA. {ECO:0000269|PubMed:18021804}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(6)-methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC Evidence={ECO:0000269|PubMed:18021804};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16498;
CC Evidence={ECO:0000269|PubMed:18021804};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18021804}.
CC -!- MISCELLANEOUS: Recombinant protein is able to methylate partially
CC deproteinized 50S ribosomal subunit, but neither the completely
CC assembled 50S subunits nor completely deproteinized 23S rRNA.
CC {ECO:0000269|PubMed:18021804}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC family. {ECO:0000305}.
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DR EMBL; U00096; AAC73894.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35473.2; -; Genomic_DNA.
DR PIR; G64817; G64817.
DR RefSeq; NP_415328.4; NC_000913.3.
DR RefSeq; WP_001295299.1; NZ_STEB01000019.1.
DR AlphaFoldDB; P75782; -.
DR SMR; P75782; -.
DR BioGRID; 4259969; 41.
DR DIP; DIP-11434N; -.
DR IntAct; P75782; 1.
DR STRING; 511145.b0807; -.
DR jPOST; P75782; -.
DR PaxDb; P75782; -.
DR PRIDE; P75782; -.
DR EnsemblBacteria; AAC73894; AAC73894; b0807.
DR EnsemblBacteria; BAA35473; BAA35473; BAA35473.
DR GeneID; 944938; -.
DR KEGG; ecj:JW5107; -.
DR KEGG; eco:b0807; -.
DR PATRIC; fig|1411691.4.peg.1471; -.
DR EchoBASE; EB3103; -.
DR eggNOG; COG3129; Bacteria.
DR HOGENOM; CLU_027534_3_0_6; -.
DR InParanoid; P75782; -.
DR OMA; HQGRYDF; -.
DR PhylomeDB; P75782; -.
DR BioCyc; EcoCyc:G6416-MON; -.
DR BioCyc; MetaCyc:G6416-MON; -.
DR BRENDA; 2.1.1.181; 2026.
DR PRO; PR:P75782; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IDA:EcoCyc.
DR GO; GO:0070475; P:rRNA base methylation; IMP:EcoCyc.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR InterPro; IPR010286; METTL16/RlmF.
DR InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR13393; PTHR13393; 1.
DR Pfam; PF05971; Methyltransf_10; 1.
DR PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..308
FT /note="Ribosomal RNA large subunit methyltransferase F"
FT /id="PRO_0000218020"
SQ SEQUENCE 308 AA; 34226 MW; E096A6624F37D546 CRC64;
MSAQKPGLHP RNRHHSRYDL ATLCQVNPEL RQFLTLTPAG EQSVDFANPL AVKALNKALL
AHFYAVANWD IPDGFLCPPV PGRADYIHHL ADLLAEASGT IPANASILDI GVGANCIYPL
IGVHEYGWRF TGSETSSQAL SSAQAIISSN PGLNRAIRLR RQKESGAIFN GIIHKNEQYD
ATLCNPPFHD SAAAARAGSE RKRRNLGLNK DDALNFGGQQ QELWCEGGEV TFIKKMIEES
KGFAKQVMWF TSLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMNDEQR
RRFVNRQR
