ID   RLMF_PSEAE              Reviewed;         336 AA.
AC   Q9HXG4;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase F {ECO:0000255|HAMAP-Rule:MF_01848};
DE            EC=2.1.1.181 {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=23S rRNA mA1618 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=rRNA adenine N-6-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
GN   Name=rlmF {ECO:0000255|HAMAP-Rule:MF_01848}; OrderedLocusNames=PA3840;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(6)-methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01848};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC       family. {ECO:0000255|HAMAP-Rule:MF_01848}.
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DR   EMBL; AE004091; AAG07227.1; -; Genomic_DNA.
DR   PIR; A83166; A83166.
DR   RefSeq; NP_252529.1; NC_002516.2.
DR   RefSeq; WP_003092887.1; NZ_QZGE01000001.1.
DR   AlphaFoldDB; Q9HXG4; -.
DR   SMR; Q9HXG4; -.
DR   STRING; 287.DR97_4026; -.
DR   PaxDb; Q9HXG4; -.
DR   PRIDE; Q9HXG4; -.
DR   DNASU; 879836; -.
DR   EnsemblBacteria; AAG07227; AAG07227; PA3840.
DR   GeneID; 879836; -.
DR   KEGG; pae:PA3840; -.
DR   PATRIC; fig|208964.12.peg.4020; -.
DR   PseudoCAP; PA3840; -.
DR   HOGENOM; CLU_027534_3_0_6; -.
DR   InParanoid; Q9HXG4; -.
DR   OMA; HQGRYDF; -.
DR   PhylomeDB; Q9HXG4; -.
DR   BioCyc; PAER208964:G1FZ6-3912-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR   InterPro; IPR010286; METTL16/RlmF.
DR   InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13393; PTHR13393; 1.
DR   Pfam; PF05971; Methyltransf_10; 1.
DR   PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..336
FT                   /note="Ribosomal RNA large subunit methyltransferase F"
FT                   /id="PRO_0000349925"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   336 AA;  37629 MW;  39176FDCAC7D679E CRC64;
     MPRPTSPHPD AERKSASPLH PRNRHLGRYD FPRLIAGSPE LERFVILNPY GRQSIDFADP
     AAVKAFNRAL LQQFYDVREW DIPDGYLCPP IPGRADYLHY LADLLGASHD GLIPRGPGLR
     ALDVGTGANC IYPLLGHHEY GWRFVGADID PQSLASAAAI LAANPRFAAA IELRRQPDRR
     QIFQGLIGVD ERFDMTLCNP PFHASLDEAT RGSRRKWKNL GKLDPTRTLP LLNFGGQGAE
     LYCEGGEAAF LAGMAEESRA FATQVFWFTT LVSKASNLPN LQERLKTLGA SDIRVVDMAQ
     GQKQSRFVAW TYLDKKQRRA WRKERWTAAL LEPLGE