ID   RLMF_VIBC3              Reviewed;         362 AA.
AC   A5F7R5; A5F7S6;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Putative ribosomal RNA large subunit methyltransferase F;
DE            EC=2.1.1.181 {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=23S rRNA mA1618 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=rRNA adenine N-6-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
GN   Name=rlmF {ECO:0000255|HAMAP-Rule:MF_01848};
GN   OrderedLocusNames=VC0395_A1219/VC0395_A1221, VC395_1730/VC395_1732;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(6)-methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01848};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC       family. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CAUTION: Could be the product of a pseudogene. Sequence is interrupted
CC       by the insertion of an IS1004 element. {ECO:0000305}.
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DR   EMBL; CP000627; ABQ19637.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP000627; ABQ21734.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP001235; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A5F7R5; -.
DR   SMR; A5F7R5; -.
DR   EnsemblBacteria; ABQ19637; ABQ19637; VC0395_A1219.
DR   EnsemblBacteria; ABQ21734; ABQ21734; VC0395_A1221.
DR   KEGG; vco:VC0395_A1219; -.
DR   KEGG; vco:VC0395_A1221; -.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR   InterPro; IPR010286; METTL16/RlmF.
DR   InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13393; PTHR13393; 1.
DR   Pfam; PF05971; Methyltransf_10; 1.
DR   PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   5: Uncertain;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..362
FT                   /note="Putative ribosomal RNA large subunit
FT                   methyltransferase F"
FT                   /id="PRO_0000349974"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..35
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   362 AA;  40449 MW;  462615840767CEC6 CRC64;
     MNTPLKPKHG QKTNQKPKAN KPVVKKQQTK QPPTHKVQGE EVAAVKSGLH PRNRHRGQYD
     FPALIKVVPE LQSHVMKNPK GQWTINFADP IAVKLLNKAL LALHYGVTYW DIPEGFLCPP
     IPGRADYIHR VADLLLKENP KLNPSQVTAL DIGVGANCIY PIVGVTEYGW SWVGSDVDPV
     SIQQASLIVQ SNSKLQGHIE FRLQKNSQHI FNGIIGANER YTLTTCNPPF HASLADAQQG
     TQRKLTNLQA NQRKKGRLAT PTLSHSRLNF GGQKAELWCP GGEAAFIGKM AVESQQFAQQ
     VLWFSTLISK GDNVRGMKKQ LEKLGAQSIH VIEMAQGQKI SRFIAWSFQN AEQRKLWWQA
     KC