RLMG_ECOLI
ID RLMG_ECOLI Reviewed; 378 AA.
AC P42596; Q2M9C2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase G;
DE EC=2.1.1.174 {ECO:0000269|PubMed:17010380};
DE AltName: Full=23S rRNA m2G1835 methyltransferase;
DE AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmG;
GN Name=rlmG; Synonyms=ygjO; OrderedLocusNames=b3084, JW5513;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION AS RRNA METHYLTRANSFERASE, AND CATALYTIC ACTIVITY.
RX PubMed=17010380; DOI=10.1016/j.jmb.2006.09.008;
RA Sergiev P.V., Lesnyak D.V., Bogdanov A.A., Dontsova O.A.;
RT "Identification of Escherichia coli m2G methyltransferases: II. The ygjO
RT gene encodes a methyltransferase specific for G1835 of the 23 S rRNA.";
RL J. Mol. Biol. 364:26-31(2006).
CC -!- FUNCTION: Specifically methylates the guanine in position 1835
CC (m2G1835) of 23S rRNA. {ECO:0000269|PubMed:17010380}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(1835) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(2)-methylguanosine(1835) in 23S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42744, Rhea:RHEA-COMP:10217, Rhea:RHEA-COMP:10218,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.174;
CC Evidence={ECO:0000269|PubMed:17010380};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42745;
CC Evidence={ECO:0000269|PubMed:17010380};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmG family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA57885.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U18997; AAA57885.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76119.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77134.1; -; Genomic_DNA.
DR PIR; A65097; A65097.
DR RefSeq; NP_417555.4; NC_000913.3.
DR RefSeq; WP_000018695.1; NZ_LN832404.1.
DR PDB; 4DCM; X-ray; 2.30 A; A=9-373.
DR PDBsum; 4DCM; -.
DR AlphaFoldDB; P42596; -.
DR SMR; P42596; -.
DR BioGRID; 4260876; 40.
DR BioGRID; 851905; 1.
DR IntAct; P42596; 1.
DR STRING; 511145.b3084; -.
DR jPOST; P42596; -.
DR PaxDb; P42596; -.
DR PRIDE; P42596; -.
DR EnsemblBacteria; AAC76119; AAC76119; b3084.
DR EnsemblBacteria; BAE77134; BAE77134; BAE77134.
DR GeneID; 947589; -.
DR KEGG; ecj:JW5513; -.
DR KEGG; eco:b3084; -.
DR PATRIC; fig|511145.12.peg.3179; -.
DR EchoBASE; EB2585; -.
DR eggNOG; COG2813; Bacteria.
DR HOGENOM; CLU_040288_4_0_6; -.
DR InParanoid; P42596; -.
DR OMA; DFLAWRM; -.
DR PhylomeDB; P42596; -.
DR BioCyc; EcoCyc:G7603-MON; -.
DR BioCyc; MetaCyc:G7603-MON; -.
DR BRENDA; 2.1.1.174; 2026.
DR PRO; PR:P42596; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052916; F:23S rRNA (guanine(1835)-N(2))-methyltransferase activity; IDA:EcoCyc.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0070475; P:rRNA base methylation; IMP:EcoCyc.
DR Gene3D; 3.40.50.150; -; 2.
DR HAMAP; MF_01859; 23SrRNA_methyltr_G; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR017237; rRNA_m2G-MeTrfase_RsmD.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR007848; Small_mtfrase_dom.
DR PANTHER; PTHR47816:SF5; PTHR47816:SF5; 1.
DR Pfam; PF05175; MTS; 1.
DR PIRSF; PIRSF037565; RRNA_m2G_Mtase_RsmD_prd; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Methyltransferase; Reference proteome;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..378
FT /note="Ribosomal RNA large subunit methyltransferase G"
FT /id="PRO_0000097492"
FT HELIX 30..37
FT /evidence="ECO:0007829|PDB:4DCM"
FT TURN 38..41
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 57..61
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 63..65
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 74..86
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 91..93
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 94..98
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 108..113
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 118..129
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 136..143
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 149..158
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 162..164
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 171..176
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 190..194
FT /evidence="ECO:0007829|PDB:4DCM"
FT TURN 195..198
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 199..203
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 215..222
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 230..236
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 241..249
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 254..260
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 262..275
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 277..282
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 283..287
FT /evidence="ECO:0007829|PDB:4DCM"
FT TURN 290..293
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 299..304
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 318..329
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 330..341
FT /evidence="ECO:0007829|PDB:4DCM"
FT HELIX 346..354
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 358..362
FT /evidence="ECO:0007829|PDB:4DCM"
FT STRAND 364..372
FT /evidence="ECO:0007829|PDB:4DCM"
SQ SEQUENCE 378 AA; 42331 MW; 0496E8BBD36FFB2C CRC64;
MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI LNDAFGALSC
ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST ADYPQQPGVV LIKVPKTLAL
LEQQLRALRK VVTSDTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFNE
PQLADAPQTV SWKLEGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG
VIGLTLLDKN PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN
AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI
ATNNKFVVLK AVKLGRRR
