ID   RLMG_PSEMY              Reviewed;         374 AA.
AC   A4XQA3;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase G {ECO:0000255|HAMAP-Rule:MF_01859};
DE            EC=2.1.1.174 {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=23S rRNA m2G1835 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmG {ECO:0000255|HAMAP-Rule:MF_01859};
GN   Name=rlmG {ECO:0000255|HAMAP-Rule:MF_01859}; OrderedLocusNames=Pmen_0751;
OS   Pseudomonas mendocina (strain ymp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=399739;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ymp;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Kiss H., Brettin T., Detter J.C., Bruce D., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Hersman L., Dubois J., Maurice P., Richardson P.;
RT   "Complete sequence of Pseudomonas mendocina ymp.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically methylates the guanine in position 1835
CC       (m2G1835) of 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(1835) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(1835) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42744, Rhea:RHEA-COMP:10217, Rhea:RHEA-COMP:10218,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.174;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01859};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmG family.
CC       {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABP83519.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000680; ABP83519.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041772801.1; NC_009439.1.
DR   AlphaFoldDB; A4XQA3; -.
DR   SMR; A4XQA3; -.
DR   STRING; 399739.Pmen_0751; -.
DR   PRIDE; A4XQA3; -.
DR   EnsemblBacteria; ABP83519; ABP83519; Pmen_0751.
DR   KEGG; pmy:Pmen_0751; -.
DR   PATRIC; fig|399739.8.peg.762; -.
DR   eggNOG; COG2813; Bacteria.
DR   HOGENOM; CLU_040288_4_0_6; -.
DR   OrthoDB; 1027015at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052916; F:23S rRNA (guanine(1835)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 2.
DR   HAMAP; MF_01859; 23SrRNA_methyltr_G; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR017237; rRNA_m2G-MeTrfase_RsmD.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR007848; Small_mtfrase_dom.
DR   PANTHER; PTHR47816:SF5; PTHR47816:SF5; 1.
DR   Pfam; PF05175; MTS; 1.
DR   PIRSF; PIRSF037565; RRNA_m2G_Mtase_RsmD_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..374
FT                   /note="Ribosomal RNA large subunit methyltransferase G"
FT                   /id="PRO_0000366478"
SQ   SEQUENCE   374 AA;  40592 MW;  EAC2DE5495969F09 CRC64;
     MPIFTTPFAS LELLRQPHQP NEPLQAFDAA DEYLLNHLHE QGLRAEDSLL LLNDSFGALA
     CSLAGRCQVT SSSDSHLGFI ALENNLAGNG LNRDAVRFLP SSETPQGPFD WVLIRVPKTL
     ALLEEQLIRL HGQLAPGARV VAAAMVKHLP RAAGDLLEKY IGPVQASLAV KKARLLLATP
     EAKAAPHSPY PTRYRLDKPA LELINHANVF CRDGLDIGTR AFLPHLPRHL DARRVADLGC
     GNGVLGIAYA LGSPQAQLTL VDESYMAVQS ARENWAAALG ERPATIRAGD GLAEQPAGSL
     DLVLCNPPFH QQQVVGDFLA WRMFQQARAA LVTGGELWIV GNRHLGYHAK LARLFRGVEQ
     VAANPKFVVL KASK