ID   RLMG_PSESH              Reviewed;         374 AA.
AC   Q0EDR2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase G {ECO:0000255|HAMAP-Rule:MF_01859};
DE            EC=2.1.1.174 {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=23S rRNA m2G1835 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmG {ECO:0000255|HAMAP-Rule:MF_01859};
GN   Name=rlmG {ECO:0000255|HAMAP-Rule:MF_01859}; ORFNames=PHA56;
OS   Pseudomonas savastanoi pv. phaseolicola (Pseudomonas syringae pv.
OS   phaseolicola).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=319;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MAFF 302282;
RX   PubMed=16927007; DOI=10.1007/s00239-005-0271-4;
RA   Genka H., Baba T., Tsuda M., Kanaya S., Mori H., Yoshida T., Noguchi M.T.,
RA   Tsuchiya K., Sawada H.;
RT   "Comparative analysis of argK-tox clusters and their flanking regions in
RT   phaseolotoxin-producing Pseudomonas syringae pathovars.";
RL   J. Mol. Evol. 63:401-414(2006).
CC   -!- FUNCTION: Specifically methylates the guanine in position 1835
CC       (m2G1835) of 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(1835) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(1835) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42744, Rhea:RHEA-COMP:10217, Rhea:RHEA-COMP:10218,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.174;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01859};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmG family.
CC       {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB237164; BAF32922.1; -; Genomic_DNA.
DR   RefSeq; WP_041924638.1; NZ_RBUR01000216.1.
DR   AlphaFoldDB; Q0EDR2; -.
DR   SMR; Q0EDR2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052916; F:23S rRNA (guanine(1835)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 2.
DR   HAMAP; MF_01859; 23SrRNA_methyltr_G; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR017237; rRNA_m2G-MeTrfase_RsmD.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR007848; Small_mtfrase_dom.
DR   PANTHER; PTHR47816:SF5; PTHR47816:SF5; 1.
DR   Pfam; PF05175; MTS; 1.
DR   PIRSF; PIRSF037565; RRNA_m2G_Mtase_RsmD_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..374
FT                   /note="Ribosomal RNA large subunit methyltransferase G"
FT                   /id="PRO_0000366484"
SQ   SEQUENCE   374 AA;  40697 MW;  DB5EDA67617EF282 CRC64;
     MPLLISPFAE LDLIRQPEQQ DEPLQAFDAA DEYLLNHVAE TGLSLQSRVL VLNDSFGALA
     ASLASHATVV SSTDSFLAAQ GLEKNLARNG MSYDAVPHIP ASEPLSGPFD WVLIRVPKTL
     ALLEEQLIRL QGQLAPGARV VAAAMVKHLP RSAGDLLEEY VGPVQASLAV KKARLLFATP
     QPMEVRTSPY PTRYRLDEPA IELLNHANVF CRDGLDIGTR AFLPYLPKNL GTARVADLGC
     GNGVLAIASA LDNPQAHYTL VDESFMAVQS AAENWRATLG ERVVEVRAAD GLDTQEPDSL
     DVVLCNPPFH QQQVVGDFLA WRMFLQARAA LVNGGALYIV GNRHLGYHTK LSRLFRGVEQ
     VAATPKFVIL KARK