ID   RLMG_STRGG              Reviewed;         384 AA.
AC   B1VMX8;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase G {ECO:0000255|HAMAP-Rule:MF_01859};
DE            EC=2.1.1.174 {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=23S rRNA m2G1835 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01859};
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmG {ECO:0000255|HAMAP-Rule:MF_01859};
GN   Name=rlmG {ECO:0000255|HAMAP-Rule:MF_01859}; OrderedLocusNames=SGR_6774;
OS   Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=455632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 4626 / NBRC 13350;
RX   PubMed=18375553; DOI=10.1128/jb.00204-08;
RA   Ohnishi Y., Ishikawa J., Hara H., Suzuki H., Ikenoya M., Ikeda H.,
RA   Yamashita A., Hattori M., Horinouchi S.;
RT   "Genome sequence of the streptomycin-producing microorganism Streptomyces
RT   griseus IFO 13350.";
RL   J. Bacteriol. 190:4050-4060(2008).
CC   -!- FUNCTION: Specifically methylates the guanine in position 1835
CC       (m2G1835) of 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(1835) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(1835) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42744, Rhea:RHEA-COMP:10217, Rhea:RHEA-COMP:10218,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.174;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01859};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01859}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmG family.
CC       {ECO:0000255|HAMAP-Rule:MF_01859}.
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DR   EMBL; AP009493; BAG23603.1; -; Genomic_DNA.
DR   RefSeq; WP_012382204.1; NC_010572.1.
DR   AlphaFoldDB; B1VMX8; -.
DR   SMR; B1VMX8; -.
DR   STRING; 455632.SGR_6774; -.
DR   PRIDE; B1VMX8; -.
DR   EnsemblBacteria; BAG23603; BAG23603; SGR_6774.
DR   GeneID; 6212127; -.
DR   KEGG; sgr:SGR_6774; -.
DR   PATRIC; fig|455632.4.peg.6953; -.
DR   eggNOG; COG2813; Bacteria.
DR   HOGENOM; CLU_040288_4_0_11; -.
DR   OMA; DFLAWRM; -.
DR   OrthoDB; 1027015at2; -.
DR   Proteomes; UP000001685; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052916; F:23S rRNA (guanine(1835)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 2.
DR   HAMAP; MF_01859; 23SrRNA_methyltr_G; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR017237; rRNA_m2G-MeTrfase_RsmD.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR007848; Small_mtfrase_dom.
DR   PANTHER; PTHR47816:SF5; PTHR47816:SF5; 1.
DR   Pfam; PF05175; MTS; 1.
DR   PIRSF; PIRSF037565; RRNA_m2G_Mtase_RsmD_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; rRNA processing; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..384
FT                   /note="Ribosomal RNA large subunit methyltransferase G"
FT                   /id="PRO_0000366529"
SQ   SEQUENCE   384 AA;  41294 MW;  166835FBE5E59C23 CRC64;
     MNRFTTSQGA FELARFPEHP RDPFRAWDAA DDYLLQQLTD PGTGPVDLSG TVAVVGDRWG
     ALATALAAHR PVQISDSYLA RRATLANLAR NGIDEDAVRL LSSRDTPPDR IDVLLVRVPK
     SLAFLEDQLH RLAPAVHSGT VVIGTGMVKE IHTSTLKIFE RIIGPTRTSL AVRKARLIFS
     TPDPALPRTL SPWPYRYELP ADVGPVAGLT AVNHAGIFCA ERLDIGTRFF LKHLPTRSGA
     VRVVDLGCGN GVLGLSAAVA NPDAHLTFVD ESYGAVASAE ETFRAGAPAG AKADFLVGDG
     LADLDPGSVD LVLNNPPFHS HLATTDATAR TMFAGAKTAL RRGGELWVVG NRHLSYHTHL
     RRIFGNCTTV AGDPKFVVLR AVKR