ID   RLMI_KLEP7              Reviewed;         400 AA.
AC   A6T766;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase I {ECO:0000255|HAMAP-Rule:MF_01857};
DE            EC=2.1.1.191 {ECO:0000255|HAMAP-Rule:MF_01857};
DE   AltName: Full=23S rRNA m5C1962 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01857};
DE   AltName: Full=rRNA (cytosine-C(5)-)-methyltransferase RlmI {ECO:0000255|HAMAP-Rule:MF_01857};
GN   Name=rlmI {ECO:0000255|HAMAP-Rule:MF_01857};
GN   OrderedLocusNames=KPN78578_09760; ORFNames=KPN_01001;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically methylates the cytosine at position 1962
CC       (m5C1962) of 23S rRNA. {ECO:0000255|HAMAP-Rule:MF_01857}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(1962) in 23S rRNA + S-adenosyl-L-methionine = 5-
CC         methylcytidine(1962) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42912, Rhea:RHEA-COMP:10382, Rhea:RHEA-COMP:10386,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74483, ChEBI:CHEBI:82748; EC=2.1.1.191;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01857};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01857}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmI family.
CC       {ECO:0000255|HAMAP-Rule:MF_01857}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABR76437.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000647; ABR76437.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_004141630.1; NC_009648.1.
DR   AlphaFoldDB; A6T766; -.
DR   SMR; A6T766; -.
DR   STRING; 272620.KPN_01001; -.
DR   jPOST; A6T766; -.
DR   PRIDE; A6T766; -.
DR   EnsemblBacteria; ABR76437; ABR76437; KPN_01001.
DR   KEGG; kpn:KPN_01001; -.
DR   HOGENOM; CLU_014042_0_0_6; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016434; F:rRNA (cytosine) methyltransferase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.130.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   HAMAP; MF_01857; 23SrRNA_methyltr_I; 1.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR041532; RlmI_PUA-like.
DR   InterPro; IPR023542; rRNA_lsu_MeTfrase_I.
DR   InterPro; IPR019614; SAM-dep_methyl-trfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF10672; Methyltrans_SAM; 1.
DR   Pfam; PF17785; PUA_3; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   rRNA processing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..400
FT                   /note="Ribosomal RNA large subunit methyltransferase I"
FT                   /id="PRO_0000366235"
FT   DOMAIN          6..84
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01857"
SQ   SEQUENCE   400 AA;  44659 MW;  7597AE058B13C95D CRC64;
     MTDSLFPRLV LAKGREKSLL RRHPWIFSGG VARMEGKARS GETIDIVDHQ GKWLARGAYS
     PSSQIRARVW TFDRNEAIDS AFFERRLQQA QTWRAWLAER DGLDSYRLIA GESDGLPGVT
     IDRFGNFFVL QLLSAGAEYQ RAAIISALQN LFPDCAIYDR SDVAVRKKEG LELAQGPVVG
     ELPPALLPIT EHGMKLLVDI QGGHKTGYYL DQRDSRLATR RYVADKRVLN CFSYTGGFAV
     SALMGGCRQV TSVDTSQEAL DVARQNVEIN GLDLSKAEFV RDDVFKLLRK YRDQGEKFDV
     IVMDPPKFVE NKSQLMGACR GYKDINMLAI QLLNPGGVLL TFSCSGLMTT DLFQKIIADA
     AIDAGRDVQF IEQFRQAADH PVIATYPEGL YLKGFACRVM