RLMKL_PSEAE
ID RLMKL_PSEAE Reviewed; 725 AA.
AC Q9HZG0;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Ribosomal RNA large subunit methyltransferase K/L {ECO:0000255|HAMAP-Rule:MF_01858};
DE Includes:
DE RecName: Full=23S rRNA m2G2445 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE EC=2.1.1.173 {ECO:0000255|HAMAP-Rule:MF_01858};
DE AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmL {ECO:0000255|HAMAP-Rule:MF_01858};
DE Includes:
DE RecName: Full=23S rRNA m7G2069 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01858};
DE EC=2.1.1.264 {ECO:0000255|HAMAP-Rule:MF_01858};
DE AltName: Full=rRNA (guanine-N(7)-)-methyltransferase RlmK {ECO:0000255|HAMAP-Rule:MF_01858};
GN Name=rlmL {ECO:0000255|HAMAP-Rule:MF_01858}; OrderedLocusNames=PA3048;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Specifically methylates the guanine in position 2445
CC (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA.
CC {ECO:0000255|HAMAP-Rule:MF_01858}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(2445) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(2)-methylguanosine(2445) in 23S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42740, Rhea:RHEA-COMP:10215, Rhea:RHEA-COMP:10216,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.173;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01858};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(2069) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC N(2)-methylguanosine(2069) in 23S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43772, Rhea:RHEA-COMP:10688, Rhea:RHEA-COMP:10689,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.264;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01858};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01858}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RlmKL family.
CC {ECO:0000255|HAMAP-Rule:MF_01858}.
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DR EMBL; AE004091; AAG06436.1; -; Genomic_DNA.
DR PIR; A83266; A83266.
DR RefSeq; NP_251738.1; NC_002516.2.
DR RefSeq; WP_003113961.1; NZ_QZGE01000009.1.
DR AlphaFoldDB; Q9HZG0; -.
DR SMR; Q9HZG0; -.
DR STRING; 287.DR97_4888; -.
DR PaxDb; Q9HZG0; -.
DR PRIDE; Q9HZG0; -.
DR EnsemblBacteria; AAG06436; AAG06436; PA3048.
DR GeneID; 882879; -.
DR KEGG; pae:PA3048; -.
DR PATRIC; fig|208964.12.peg.3198; -.
DR PseudoCAP; PA3048; -.
DR HOGENOM; CLU_014042_2_0_6; -.
DR InParanoid; Q9HZG0; -.
DR OMA; WIRGYEA; -.
DR PhylomeDB; Q9HZG0; -.
DR BioCyc; PAER208964:G1FZ6-3101-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0052915; F:23S rRNA (guanine(2445)-N(2))-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008990; F:rRNA (guanine-N2-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 2.
DR HAMAP; MF_01858; 23SrRNA_methyltr_KL; 1.
DR InterPro; IPR017244; 23SrRNA_methyltr_KL.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RNA_methylase_dom.
DR InterPro; IPR019614; SAM-dep_methyl-trfase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR004114; THUMP_dom.
DR Pfam; PF10672; Methyltrans_SAM; 1.
DR Pfam; PF02926; THUMP; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF037618; RNA_Mtase_bacteria_prd; 1.
DR SMART; SM00981; THUMP; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
DR PROSITE; PS51165; THUMP; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..725
FT /note="Ribosomal RNA large subunit methyltransferase K/L"
FT /id="PRO_0000366785"
FT DOMAIN 46..157
FT /note="THUMP"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01858"
SQ SEQUENCE 725 AA; 81155 MW; 627B3A2CC86A8BE0 CRC64;
MADVYELFLT CPKGLESLLL EEAQGLGLDE ARAQVSAVRG QGSLEVAYRL CLWSRLANRV
LLVLARFPVE NAESMYMAVH AVNWEDHLDA GGTLAVEFSG KGSGIDNTHF GALKVKDAIV
DNLRERSGRR PSVDKVNPDV RVHLHLDRGQ ATLSLDLSGH SLHQRGYRLQ QGAAPLKENL
AAAVLIRAGW PKIAAEGGAL ADPMCGVGTF LVEAALMAAD IAPNLKRERW GFSSWLGHVP
ALWRKLHEEA QQRAAAGLAR PPLWIRGYEA DPRLIQPACN NIERAGVADW VKIYQGELAT
FEPRPDKGQT GLVICNPPYG ERLGDEASLL YLYQNLGERL RQSCIGWSAG VFTGAPELGK
RMGIRSHKQY AFWNGALACK LLMIQVEPRQ FVTGERGERN DEGLARAPSE PARLSEGGQM
FANRLQKNLR QLGKWARRDK IECYRLYDAD MPEYALAVDI YGDWVHVQEY AAPKSIDPAK
AQARLFDALA AIPQTLGVAQ ERVVVKRRER QAGKKQYERQ SSEGKFLEVG EGDVRLLVNL
TDYLDTGLFL DHRPMRLRIQ KEAAGKRFLN LFCYTATATV HAARGGARST TSVDLSKTYL
DWARRNLSLN GFSDKQRLVH GDVMEWLRED DGQYELIFID PPTFSNSKRM EGVFDVQRDH
VELLDLAMAR LAPGGVLYFS NNFRKFELDE SVQARYAVEE ITGETLDPDF ARNPKIHRAW
RITVR
