ID   RLMK_NEIMB              Reviewed;         354 AA.
AC   Q9JYY8;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase K;
DE            EC=2.1.1.264;
DE   AltName: Full=23S rRNA m7G2069 methyltransferase;
DE   AltName: Full=rRNA (guanine-N(7)-)-methyltransferase RlmK;
GN   Name=rlmK; OrderedLocusNames=NMB1367;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=22210896; DOI=10.1093/nar/gkr1287;
RA   Kimura S., Ikeuchi Y., Kitahara K., Sakaguchi Y., Suzuki T., Suzuki T.;
RT   "Base methylations in the double-stranded RNA by a fused methyltransferase
RT   bearing unwinding activity.";
RL   Nucleic Acids Res. 40:4071-4085(2012).
CC   -!- FUNCTION: Specifically methylates the guanine in position 2069
CC       (m7G2069) of 23S rRNA. {ECO:0000269|PubMed:22210896}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(2069) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(2069) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43772, Rhea:RHEA-COMP:10688, Rhea:RHEA-COMP:10689,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.264;
CC         Evidence={ECO:0000269|PubMed:22210896};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE002098; AAF41741.1; -; Genomic_DNA.
DR   PIR; D81090; D81090.
DR   RefSeq; NP_274385.1; NC_003112.2.
DR   AlphaFoldDB; Q9JYY8; -.
DR   SMR; Q9JYY8; -.
DR   STRING; 122586.NMB1367; -.
DR   PaxDb; Q9JYY8; -.
DR   EnsemblBacteria; AAF41741; AAF41741; NMB1367.
DR   KEGG; nme:NMB1367; -.
DR   PATRIC; fig|122586.8.peg.1711; -.
DR   HOGENOM; CLU_014042_1_1_4; -.
DR   OMA; LMKWAKR; -.
DR   BRENDA; 2.1.1.264; 3593.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR019614; SAM-dep_methyl-trfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF10672; Methyltrans_SAM; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   rRNA processing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..354
FT                   /note="Ribosomal RNA large subunit methyltransferase K"
FT                   /id="PRO_0000419971"
SQ   SEQUENCE   354 AA;  41086 MW;  C822861032B3C8C3 CRC64;
     MASYDKISDG WYRVCPKRSS NRALITVKLP FSTLFRLKPM TDITPFANRL GKNIKHLMKW
     AKRNGIEAWR IYDRDIPQFP FAADVYGDRI HLQEYDTGWL MRPEEYEAWL AEVLEAVAFV
     TGFAPEQIRL KRRERQKGLQ QYEKTGKAGD DFVITENGRK FWVNLDKYLD TGLFLDHRNT
     RKKVGETAAG KRFLNLFSYT GSFTVYAATG GAASSETVDL SNTYLDWAKR NFELNGIDTE
     RHKIVRADVF QYLQTAYGEG RRFDLIVMDP PSFSNSKKMS DILDIQRDHK KLIDGAVKLL
     ASDGILYFSN NLRSFVLDDL VSEQYAVKDI SKQSVPEDFR NKKIHRCWEI RHKS