ID   RLML_NEIMB              Reviewed;         380 AA.
AC   Q9K0V4;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase L;
DE            EC=2.1.1.173;
DE   AltName: Full=23S rRNA m2G2445 methyltransferase;
DE   AltName: Full=rRNA (guanine-N(2)-)-methyltransferase RlmL;
GN   Name=rlmL; OrderedLocusNames=NMB0455;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=22210896; DOI=10.1093/nar/gkr1287;
RA   Kimura S., Ikeuchi Y., Kitahara K., Sakaguchi Y., Suzuki T., Suzuki T.;
RT   "Base methylations in the double-stranded RNA by a fused methyltransferase
RT   bearing unwinding activity.";
RL   Nucleic Acids Res. 40:4071-4085(2012).
CC   -!- FUNCTION: Specifically methylates the guanine in position 2445
CC       (m2G2445) of 23S rRNA. {ECO:0000269|PubMed:22210896}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(2445) in 23S rRNA + S-adenosyl-L-methionine = H(+) +
CC         N(2)-methylguanosine(2445) in 23S rRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42740, Rhea:RHEA-COMP:10215, Rhea:RHEA-COMP:10216,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.173;
CC         Evidence={ECO:0000269|PubMed:22210896};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE002098; AAF40892.1; -; Genomic_DNA.
DR   PIR; A81198; A81198.
DR   RefSeq; NP_273502.1; NC_003112.2.
DR   RefSeq; WP_002224938.1; NC_003112.2.
DR   AlphaFoldDB; Q9K0V4; -.
DR   SMR; Q9K0V4; -.
DR   STRING; 122586.NMB0455; -.
DR   PaxDb; Q9K0V4; -.
DR   DNASU; 902571; -.
DR   EnsemblBacteria; AAF40892; AAF40892; NMB0455.
DR   KEGG; nme:NMB0455; -.
DR   PATRIC; fig|122586.8.peg.578; -.
DR   HOGENOM; CLU_032119_3_0_4; -.
DR   OMA; GFMYKAN; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052915; F:23S rRNA (guanine(2445)-N(2))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016423; F:tRNA (guanine) methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR004114; THUMP_dom.
DR   Pfam; PF02926; THUMP; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   SMART; SM00981; THUMP; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51165; THUMP; 1.
DR   PROSITE; PS01261; UPF0020; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   rRNA processing; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..380
FT                   /note="Ribosomal RNA large subunit methyltransferase L"
FT                   /id="PRO_0000419972"
FT   DOMAIN          46..157
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
SQ   SEQUENCE   380 AA;  42664 MW;  735727D9F504204D CRC64;
     MNTLYTLFAT CPRGLETVLS QELESLGCTD VQVFDGGVSC RGGLEQVYAA NLHSRTASRI
     LLRLTKGTYR NERDIYKLAK NINWFNWFTL QQTFKVKVEA KRANVKSIQF VGLTVKDAVC
     DAFRDIYDAR PSVDKAAPDV RIHAFLNERN VEIFIDTSGE ALFKRGYRLD TGEAPLRENL
     AAGLLLSAGY DGTQPFQDPF CGSGTIAIEA AWIAARRAPG MMRRFGFEKL QNFDKTLWSD
     LRRRAEAQTR PVRAPIAGSD NDRRIVQTAL DNARRAGVDD IVSFSVADAQ SVRPNGENGI
     MVSNPPYGVR LEEVRALQAL YPQLGTWLKK HYAGWLAAMF TGDREMPKFM CLSPKRKIPL
     YNGNIDCRLF LIDMVEGSNR