AB17A_MOUSE
ID AB17A_MOUSE Reviewed; 310 AA.
AC Q99JW1; Q5DU54;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Alpha/beta hydrolase domain-containing protein 17A {ECO:0000305};
DE Short=Abhydrolase domain-containing protein 17A {ECO:0000312|MGI:MGI:106388};
DE EC=3.1.2.22 {ECO:0000250|UniProtKB:Q96GS6};
GN Name=Abhd17a {ECO:0000312|MGI:MGI:106388}; Synonyms=D10Bwg1364e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Hydrolyzes fatty acids from S-acylated cysteine residues in
CC proteins. Has depalmitoylating activity towards NRAS. Has
CC depalmitoylating activity towards DLG4/PSD95. May have depalmitoylating
CC activity towars MAP6. {ECO:0000250|UniProtKB:Q5XIJ5,
CC ECO:0000250|UniProtKB:Q96GS6}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + S-hexadecanoyl-L-cysteinyl-[protein] = H(+) +
CC hexadecanoate + L-cysteinyl-[protein]; Xref=Rhea:RHEA:19233,
CC Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:7896,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:74151; EC=3.1.2.22;
CC Evidence={ECO:0000250|UniProtKB:Q96GS6};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96GS6};
CC Lipid-anchor {ECO:0000250|UniProtKB:Q96GS6}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q96GS6}. Endosome membrane
CC {ECO:0000250|UniProtKB:Q96GS6}; Lipid-anchor
CC {ECO:0000250|UniProtKB:Q96GS6}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q96GS6}. Cell projection, dendritic spine
CC {ECO:0000250|UniProtKB:Q5XIJ5}. Postsynaptic density membrane
CC {ECO:0000250|UniProtKB:Q5XIJ5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99JW1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99JW1-2; Sequence=VSP_027272;
CC -!- PTM: Palmitoylated on cysteine residues located in a cysteine cluster
CC at the N-terminus which promotes membrane localization. Palmitoylation
CC is required for post-synaptic localization and for depalmitoylating
CC activity towards DLG4/PSD95. {ECO:0000250|UniProtKB:Q7M759}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. ABHD17 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90391.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK220316; BAD90391.1; ALT_INIT; Transcribed_RNA.
DR EMBL; BC005632; AAH05632.1; -; mRNA.
DR EMBL; BC082997; AAH82997.1; -; mRNA.
DR CCDS; CCDS24027.1; -. [Q99JW1-1]
DR RefSeq; NP_663396.1; NM_145421.2. [Q99JW1-1]
DR RefSeq; XP_017169383.1; XM_017313894.1. [Q99JW1-1]
DR AlphaFoldDB; Q99JW1; -.
DR SMR; Q99JW1; -.
DR BioGRID; 229715; 1.
DR STRING; 10090.ENSMUSP00000003436; -.
DR ESTHER; mouse-Q99JW1; ABHD17-depalmitoylase.
DR MEROPS; S09.052; -.
DR iPTMnet; Q99JW1; -.
DR PhosphoSitePlus; Q99JW1; -.
DR SwissPalm; Q99JW1; -.
DR EPD; Q99JW1; -.
DR MaxQB; Q99JW1; -.
DR PaxDb; Q99JW1; -.
DR PRIDE; Q99JW1; -.
DR ProteomicsDB; 285702; -. [Q99JW1-1]
DR ProteomicsDB; 285703; -. [Q99JW1-2]
DR Antibodypedia; 53691; 49 antibodies from 15 providers.
DR DNASU; 216169; -.
DR Ensembl; ENSMUST00000003436; ENSMUSP00000003436; ENSMUSG00000003346. [Q99JW1-1]
DR GeneID; 216169; -.
DR KEGG; mmu:216169; -.
DR UCSC; uc007gdv.2; mouse. [Q99JW1-1]
DR CTD; 81926; -.
DR MGI; MGI:106388; Abhd17a.
DR VEuPathDB; HostDB:ENSMUSG00000003346; -.
DR eggNOG; KOG1552; Eukaryota.
DR GeneTree; ENSGT00940000155854; -.
DR HOGENOM; CLU_029375_5_4_1; -.
DR InParanoid; Q99JW1; -.
DR OMA; NDIVCMY; -.
DR OrthoDB; 691954at2759; -.
DR PhylomeDB; Q99JW1; -.
DR TreeFam; TF314365; -.
DR Reactome; R-MMU-9648002; RAS processing.
DR BioGRID-ORCS; 216169; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Abhd17a; mouse.
DR PRO; PR:Q99JW1; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q99JW1; protein.
DR Bgee; ENSMUSG00000003346; Expressed in external carotid artery and 247 other tissues.
DR ExpressionAtlas; Q99JW1; baseline and differential.
DR Genevisible; Q99JW1; MM.
DR GO; GO:0099031; C:anchored component of postsynaptic density membrane; ISO:MGI.
DR GO; GO:0099033; C:anchored component of postsynaptic recycling endosome membrane; ISO:MGI.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0043197; C:dendritic spine; IEA:UniProtKB-SubCell.
DR GO; GO:0010008; C:endosome membrane; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0014069; C:postsynaptic density; ISO:MGI.
DR GO; GO:0055038; C:recycling endosome membrane; ISO:MGI.
DR GO; GO:0008474; F:palmitoyl-(protein) hydrolase activity; ISO:MGI.
DR GO; GO:1902817; P:negative regulation of protein localization to microtubule; ISO:MGI.
DR GO; GO:1905668; P:positive regulation of protein localization to endosome; ISO:MGI.
DR GO; GO:0002084; P:protein depalmitoylation; ISO:MGI.
DR GO; GO:0072657; P:protein localization to membrane; ISO:MGI.
DR GO; GO:0099175; P:regulation of postsynapse organization; ISO:MGI.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR022742; Hydrolase_4.
DR Pfam; PF12146; Hydrolase_4; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cell projection; Endosome; Hydrolase;
KW Lipoprotein; Membrane; Palmitate; Phosphoprotein;
KW Postsynaptic cell membrane; Reference proteome; Synapse.
FT CHAIN 1..310
FT /note="Alpha/beta hydrolase domain-containing protein 17A"
FT /id="PRO_0000297510"
FT REGION 38..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 190
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q96GS6"
FT ACT_SITE 255
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:O75608"
FT ACT_SITE 284
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:O75608"
FT MOD_RES 307
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96GS6"
FT VAR_SEQ 112..175
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_027272"
SQ SEQUENCE 310 AA; 33950 MW; 7152DE76D55F5189 CRC64;
MNGLSVSELC CLFCCPPCPG RIAAKLAFLP PDPTYSLVPE PEPGPGGAGA APSGPLRTSA
ATPGRWKIHL TERADFQYGQ RELDTIEVFV TKSARANRIA CMYVRCVPGA RYTVLFSHGN
AVDLGQMCSF YVGLGTRIGC NIFSYDYSGY GISSGRPSEK NLYADIDAAW QALRTRYGIS
PDSIILYGQS IGTVPTVDLA SRYECAAVVL HSPLTSGMRV AFPDTKKTYC FDAFPNIEKV
SKITSPVLII HGTEDEVIDF SHGLALYERC PKAVEPLWVE GAGHNDIELY SQYLERLRRF
ISQELPSQRA
