ID   RLR04_PLAHL             Reviewed;         119 AA.
AC   A0A0P1B6Y2;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C04 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C04 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that suppresses pattern-triggered immunity
CC       (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host
CC       cytoplasm {ECO:0000269|PubMed:30422341}. Host nucleus
CC       {ECO:0000269|PubMed:30422341}.
CC   -!- INDUCTION: Expression is up-regulated in spores.
CC       {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01003055; CEG49414.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0P1B6Y2; -.
DR   SMR; A0A0P1B6Y2; -.
DR   EnsemblProtists; CEG49414; CEG49414; CEG49414.
DR   OrthoDB; 1570398at2759; -.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031825; RXLR.
DR   Pfam; PF16810; RXLR; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host cytoplasm; Host nucleus; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..119
FT                   /note="Secreted RxLR effector protein RXLR-C04"
FT                   /id="PRO_5006059233"
FT   MOTIF           40..77
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:30422341"
FT   CARBOHYD        46
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   119 AA;  13460 MW;  46A50765B2B6430D CRC64;
     MRLSYIFVVV ATIITNCDIA SASLRAIMSD TASGNGLGTR ILRQTNDSDD LEPIRHAMLD
     MELLEKIAKD PKYAEEVFGN WRHNGQTKAE MENRLQSNGL LGKYRFIIDR YAEHLANSE