ID   RLR08_PLAHL             Reviewed;         503 AA.
AC   A0A0P1B5K4;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C08 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C08 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that suppresses pattern-triggered immunity
CC       (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host
CC       Golgi apparatus {ECO:0000269|PubMed:30422341}. Note=Localizes also to
CC       host P-bodies. {ECO:0000269|PubMed:30422341}.
CC   -!- INDUCTION: Expression is up-regulated in spores.
CC       {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: Has the canonical dEER motif, but lacks translocation RxLR
CC       motif, which characterizes most oomycete effectors identified so far.
CC       {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01003055; CEG49461.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0P1B5K4; -.
DR   SMR; A0A0P1B5K4; -.
DR   EnsemblProtists; CEG49461; CEG49461; CEG49461.
DR   OMA; SCETANC; -.
DR   OrthoDB; 1469062at2759; -.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host Golgi apparatus; Reference proteome; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..503
FT                   /note="Secreted RxLR effector protein RXLR-C08"
FT                   /id="PRO_5006059196"
FT   MOTIF           57..60
FT                   /note="dEER"
FT                   /evidence="ECO:0000305|PubMed:30422341"
FT   CARBOHYD        27
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        35
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        45
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        108
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        197
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        374
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   503 AA;  56185 MW;  CAE5961E5FBDE4FC CRC64;
     MRLCGVASAF LSTLILIAHI DASTNLNVSV TDVQNISLPG DQIGNFTQSV SGPPSGDEER
     TSANSAIRKV DLFVEKLITF QVALRAKINR FLAKLKLAWW RLRNVDPNVT FNKLNLHEAD
     NDILSLDNFH TWVKLVESYN LQHPAEQVSI LSKLQEQFGE FEVSIMLEQA KNGADKYTED
     IALELQHEQI SRWRDDNLSL TALYKALQFG KSEPSLLTGP ALRVWNIYTS NIESAETSLF
     DYLYQTIEDA HLSSLLIAAK QSPETVELAT KIQNQLRQKW LEILVPPNLV FQHYKLDTNP
     THLLDRPETK LWVRYQKMYW GKTKKEVTLK EMIEDFYKAD EIAIIIKSAT TDYGKHLAKK
     LPQCDSKHFK TFHNLTILPL LFVFHYTIGA EDDLSSEKNL ISQYNHVPTS CETANCVAGG
     CLFENCASPL LCRGGLCYFR KCKDATCEGG ACIFDNTAEA SCPGGGCQFV NVPVTLADGY
     CDGGGCTLDG DDHPSSLAGS LAE