ID   RLR12_PLAHL             Reviewed;         173 AA.
AC   A0A0P1AZU2;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C12 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C12 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that suppresses pattern-triggered immunity
CC       (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host
CC       cytoplasm {ECO:0000269|PubMed:30422341}. Host nucleus
CC       {ECO:0000269|PubMed:30422341}.
CC   -!- INDUCTION: Expression is up-regulated during the late plant infection
CC       stages. {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01002371; CEG47148.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0P1AZU2; -.
DR   EnsemblProtists; CEG47148; CEG47148; CEG47148.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host cytoplasm; Host nucleus; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..173
FT                   /note="Secreted RxLR effector protein RXLR-C12"
FT                   /id="PRO_5006059076"
FT   MOTIF           41..55
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:30422341"
FT   CARBOHYD        155
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   173 AA;  19509 MW;  1A12080CC3126A7B CRC64;
     MLQFATAFLA ISANVVMTQP NQEYIAPGAR VSSNYRDMTI RRLRTHEIGT VPEERMPIHE
     LEIEDLITII ASKVVPELSH SESMSLLSAE AARSRSFKDM AADELDVDGA LDLLKAVQSK
     PETTDMTPTF GVWEESNFDS ATVEKLFDSK KNDENESHIK TIVEAYKKFR KEK