ID   RLR21_PLAHL             Reviewed;         123 AA.
AC   A0A0P1ATX0;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C21 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C21 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that does not suppress pattern-triggered
CC       immunity (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host
CC       endoplasmic reticulum membrane {ECO:0000269|PubMed:30422341}; Single-
CC       pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is up-regulated during the late plant infection
CC       stages. {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01001640; CEG45727.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0P1ATX0; -.
DR   EnsemblProtists; CEG45727; CEG45727; CEG45727.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host endoplasmic reticulum; Host membrane; Membrane; Reference proteome;
KW   Secreted; Signal; Transmembrane; Transmembrane helix; Virulence.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..123
FT                   /note="Secreted RxLR effector protein RXLR-C21"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5006058920"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           32..65
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:30422341"
SQ   SEQUENCE   123 AA;  13942 MW;  AC611B08842CE410 CRC64;
     MRLHLLVLSV IVVSLLVSDN AHANSHDDSK TRALRETPIN GLVTNQLAVS RNLTPAKFIT
     NSEERHSSEK KSRRLQIYFH SPYYGIHPVD YHYVGSYESG VTTICSIVLF VMVFGCLYKI
     FSQ