ID   RLR28_PLAHL             Reviewed;         203 AA.
AC   A0A0N7L4N2;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Secreted RxLR effector protein RXLR-C28 {ECO:0000303|PubMed:30422341};
DE   Flags: Precursor;
GN   Name=RXLR-C28 {ECO:0000303|PubMed:30422341};
OS   Plasmopara halstedii (Downy mildew of sunflower).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Plasmopara.
OX   NCBI_TaxID=4781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=26438312; DOI=10.1186/s12864-015-1904-7;
RA   Sharma R., Xia X., Cano L.M., Evangelisti E., Kemen E., Judelson H.,
RA   Oome S., Sambles C., van den Hoogen D.J., Kitner M., Klein J., Meijer H.J.,
RA   Spring O., Win J., Zipper R., Bode H.B., Govers F., Kamoun S.,
RA   Schornack S., Studholme D.J., Van den Ackerveken G., Thines M.;
RT   "Genome analyses of the sunflower pathogen Plasmopara halstedii provide
RT   insights into effector evolution in downy mildews and Phytophthora.";
RL   BMC Genomics 16:741-741(2015).
RN   [2]
RP   DOMAIN, INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30422341; DOI=10.1111/tpj.14157;
RA   Pecrix Y., Buendia L., Penouilh-Suzette C., Marechaux M., Legrand L.,
RA   Bouchez O., Rengel D., Gouzy J., Cottret L., Vear F., Godiard L.;
RT   "Sunflower resistance to multiple downy mildew pathotypes revealed by
RT   recognition of conserved effectors of the oomycete Plasmopara halstedii.";
RL   Plant J. 97:730-748(2019).
CC   -!- FUNCTION: Secreted effector that does not suppress pattern-triggered
CC       immunity (PTI) in plant host. {ECO:0000269|PubMed:30422341}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30422341}. Host
CC       cytoplasm {ECO:0000269|PubMed:30422341}.
CC   -!- INDUCTION: Expression is up-regulated during the late plant infection
CC       stages. {ECO:0000269|PubMed:30422341}.
CC   -!- DOMAIN: Has the canonical translocation RxLR motif, but lacks the
CC       canonical EER motif, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:30422341}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; CCYD01000349; CEG39147.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0N7L4N2; -.
DR   EnsemblProtists; CEG39147; CEG39147; CEG39147.
DR   OMA; VKFEAVM; -.
DR   OrthoDB; 1359791at2759; -.
DR   Proteomes; UP000054928; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host cytoplasm; Reference proteome; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..203
FT                   /note="Secreted RxLR effector protein RXLR-C28"
FT                   /id="PRO_5006015048"
FT   MOTIF           37..40
FT                   /note="RxLR"
FT                   /evidence="ECO:0000305|PubMed:30422341"
FT   CARBOHYD        32
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        193
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   203 AA;  22674 MW;  0C37D8374078F26C CRC64;
     MKAVKLTAAV VVLFMAPYVP ITSSRSLAET PNDSIDRHLR HESAQIDSKL KEVEGKHDSN
     TPLQRRDQTL VAAHRVYDPV SGLACSLVGE CMSCPITEKD ESFCRETGYR QELACPHSKD
     EALLQTLEEK QTPRYRPCSP ADTVRPGVTF VKFEAIMSLL LAISVTLLRR EREKHMSSFD
     LRKDSRQRVG LLNSSASGVK DSD